Bio-synthesis of citric acid from single and co-culture-based fermentation technology using agro-wastes
Agro-based materials are primarily composed of complex polysaccharides that strengthen microbial growth for the production of industrially relevant value-added products. Therefore, in the present study, solid state fermentation (SSF) was carried out using agro-based waste materials (apple pomace, pe...
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doaj-aaa86ed3e2f14721bb3eaf33c3ddee6c2020-11-24T21:55:18ZengTaylor & Francis GroupJournal of Radiation Research and Applied Sciences1687-85072016-01-0191576210.1016/j.jrras.2015.09.003Bio-synthesis of citric acid from single and co-culture-based fermentation technology using agro-wastesSohaib Rafaqat AliZahid AnwarMuhammad IrshadSaima MukhtarNabeela Tariq WarraichAgro-based materials are primarily composed of complex polysaccharides that strengthen microbial growth for the production of industrially relevant value-added products. Therefore, in the present study, solid state fermentation (SSF) was carried out using agro-based waste materials (apple pomace, peanut shell and a mixture of both apple pomace and peanut shell with 50:50 ratio) as carriers/support for SSF to enhance citric acid production from single and co-culture consortia of Aspergillus ornatus and Alternaria alternata. During initial screening trial it was observed that growth media supplemented with apple pomace under SSF process of co-culture consortia presenting the preeminent 0.46 ± 0.42 mg/mL of citric acid. On partial optimization co-culture showed the maximum citric acid yield (2.644 ± 0.99 mg/mL) in the presence of arginine as a nutritional ingredient at 30 °C in an apple pomace based medium at 50% moisture content with pH of 5 and substrate concentration (25 g) after 48th of solid state fermentation. In conclusion, a suitable addition of fermentative substrate to the SSF medium increased fungal growth, sugar utilization and citric acid production when used in lower concentrations.http://www.sciencedirect.com/science/article/pii/S1687850715001004Citric acidSSFOptimizationAspergillus ornatusAlternaria alternata |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sohaib Rafaqat Ali Zahid Anwar Muhammad Irshad Saima Mukhtar Nabeela Tariq Warraich |
spellingShingle |
Sohaib Rafaqat Ali Zahid Anwar Muhammad Irshad Saima Mukhtar Nabeela Tariq Warraich Bio-synthesis of citric acid from single and co-culture-based fermentation technology using agro-wastes Journal of Radiation Research and Applied Sciences Citric acid SSF Optimization Aspergillus ornatus Alternaria alternata |
author_facet |
Sohaib Rafaqat Ali Zahid Anwar Muhammad Irshad Saima Mukhtar Nabeela Tariq Warraich |
author_sort |
Sohaib Rafaqat Ali |
title |
Bio-synthesis of citric acid from single and co-culture-based fermentation technology using agro-wastes |
title_short |
Bio-synthesis of citric acid from single and co-culture-based fermentation technology using agro-wastes |
title_full |
Bio-synthesis of citric acid from single and co-culture-based fermentation technology using agro-wastes |
title_fullStr |
Bio-synthesis of citric acid from single and co-culture-based fermentation technology using agro-wastes |
title_full_unstemmed |
Bio-synthesis of citric acid from single and co-culture-based fermentation technology using agro-wastes |
title_sort |
bio-synthesis of citric acid from single and co-culture-based fermentation technology using agro-wastes |
publisher |
Taylor & Francis Group |
series |
Journal of Radiation Research and Applied Sciences |
issn |
1687-8507 |
publishDate |
2016-01-01 |
description |
Agro-based materials are primarily composed of complex polysaccharides that strengthen microbial growth for the production of industrially relevant value-added products. Therefore, in the present study, solid state fermentation (SSF) was carried out using agro-based waste materials (apple pomace, peanut shell and a mixture of both apple pomace and peanut shell with 50:50 ratio) as carriers/support for SSF to enhance citric acid production from single and co-culture consortia of Aspergillus ornatus and Alternaria alternata. During initial screening trial it was observed that growth media supplemented with apple pomace under SSF process of co-culture consortia presenting the preeminent 0.46 ± 0.42 mg/mL of citric acid. On partial optimization co-culture showed the maximum citric acid yield (2.644 ± 0.99 mg/mL) in the presence of arginine as a nutritional ingredient at 30 °C in an apple pomace based medium at 50% moisture content with pH of 5 and substrate concentration (25 g) after 48th of solid state fermentation. In conclusion, a suitable addition of fermentative substrate to the SSF medium increased fungal growth, sugar utilization and citric acid production when used in lower concentrations. |
topic |
Citric acid SSF Optimization Aspergillus ornatus Alternaria alternata |
url |
http://www.sciencedirect.com/science/article/pii/S1687850715001004 |
work_keys_str_mv |
AT sohaibrafaqatali biosynthesisofcitricacidfromsingleandcoculturebasedfermentationtechnologyusingagrowastes AT zahidanwar biosynthesisofcitricacidfromsingleandcoculturebasedfermentationtechnologyusingagrowastes AT muhammadirshad biosynthesisofcitricacidfromsingleandcoculturebasedfermentationtechnologyusingagrowastes AT saimamukhtar biosynthesisofcitricacidfromsingleandcoculturebasedfermentationtechnologyusingagrowastes AT nabeelatariqwarraich biosynthesisofcitricacidfromsingleandcoculturebasedfermentationtechnologyusingagrowastes |
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