miRNAome expression profiles in the gonads of adult Melopsittacus undulatus
The budgerigar (Melopsittacus undulatus) is one of the most widely studied parrot species, serving as an excellent animal model for behavior and neuroscience research. Until recently, it was unknown how sexual differences in the behavior, physiology, and development of organisms are regulated by dif...
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doaj-aac01443181e4e6eba4b5a6801aa14892020-11-25T00:37:07ZengPeerJ Inc.PeerJ2167-83592018-04-016e461510.7717/peerj.4615miRNAome expression profiles in the gonads of adult Melopsittacus undulatusLan Jiang0Qingqing Wang1Jue Yu2Vinita Gowda3Gabriel Johnson4Jianke Yang5Xianzhao Kan6Xiaojun Yang7State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, ChinaThe Institute of Bioinformatics, College of Life Sciences, Anhui Normal University, Wuhu, ChinaCollege of Foreign Studies, Anhui Normal University, Wuhu, ChinaDepartment of Biological Sciences, Indian Institute of Science Education and Research, Bhopal, Madhya Pradesh, IndiaDepartment of Botany, National Museum of Natural History, Smithsonian Institution, Washington, District of Columbia, USASchool of Basic Medicine, Wannan Medical College, Wuhu, ChinaThe Institute of Bioinformatics, College of Life Sciences, Anhui Normal University, Wuhu, ChinaState Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, ChinaThe budgerigar (Melopsittacus undulatus) is one of the most widely studied parrot species, serving as an excellent animal model for behavior and neuroscience research. Until recently, it was unknown how sexual differences in the behavior, physiology, and development of organisms are regulated by differential gene expression. MicroRNAs (miRNAs) are endogenous short non-coding RNA molecules that can post-transcriptionally regulate gene expression and play a critical role in gonadal differentiation as well as early development of animals. However, very little is known about the role gonadal miRNAs play in the early development of birds. Research on the sex-biased expression of miRNAs in avian gonads are limited, and little is known about M. undulatus. In the current study, we sequenced two small non-coding RNA libraries made from the gonads of adult male and female budgerigars using Illumina paired-end sequencing technology. We obtained 254 known and 141 novel miRNAs, and randomly validated five miRNAs. Of these, three miRNAs were differentially expressed miRNAs and 18 miRNAs involved in sexual differentiation as determined by functional analysis with GO annotation and KEGG pathway analysis. In conclusion, this work is the first report of sex-biased miRNAs expression in the budgerigar, and provides additional sequences to the avian miRNAome database which will foster further functional genomic research.https://peerj.com/articles/4615.pdfGonadsSex-biasedmiRNAAdultMelopsittacus undulatus |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lan Jiang Qingqing Wang Jue Yu Vinita Gowda Gabriel Johnson Jianke Yang Xianzhao Kan Xiaojun Yang |
spellingShingle |
Lan Jiang Qingqing Wang Jue Yu Vinita Gowda Gabriel Johnson Jianke Yang Xianzhao Kan Xiaojun Yang miRNAome expression profiles in the gonads of adult Melopsittacus undulatus PeerJ Gonads Sex-biased miRNA Adult Melopsittacus undulatus |
author_facet |
Lan Jiang Qingqing Wang Jue Yu Vinita Gowda Gabriel Johnson Jianke Yang Xianzhao Kan Xiaojun Yang |
author_sort |
Lan Jiang |
title |
miRNAome expression profiles in the gonads of adult Melopsittacus undulatus |
title_short |
miRNAome expression profiles in the gonads of adult Melopsittacus undulatus |
title_full |
miRNAome expression profiles in the gonads of adult Melopsittacus undulatus |
title_fullStr |
miRNAome expression profiles in the gonads of adult Melopsittacus undulatus |
title_full_unstemmed |
miRNAome expression profiles in the gonads of adult Melopsittacus undulatus |
title_sort |
mirnaome expression profiles in the gonads of adult melopsittacus undulatus |
publisher |
PeerJ Inc. |
series |
PeerJ |
issn |
2167-8359 |
publishDate |
2018-04-01 |
description |
The budgerigar (Melopsittacus undulatus) is one of the most widely studied parrot species, serving as an excellent animal model for behavior and neuroscience research. Until recently, it was unknown how sexual differences in the behavior, physiology, and development of organisms are regulated by differential gene expression. MicroRNAs (miRNAs) are endogenous short non-coding RNA molecules that can post-transcriptionally regulate gene expression and play a critical role in gonadal differentiation as well as early development of animals. However, very little is known about the role gonadal miRNAs play in the early development of birds. Research on the sex-biased expression of miRNAs in avian gonads are limited, and little is known about M. undulatus. In the current study, we sequenced two small non-coding RNA libraries made from the gonads of adult male and female budgerigars using Illumina paired-end sequencing technology. We obtained 254 known and 141 novel miRNAs, and randomly validated five miRNAs. Of these, three miRNAs were differentially expressed miRNAs and 18 miRNAs involved in sexual differentiation as determined by functional analysis with GO annotation and KEGG pathway analysis. In conclusion, this work is the first report of sex-biased miRNAs expression in the budgerigar, and provides additional sequences to the avian miRNAome database which will foster further functional genomic research. |
topic |
Gonads Sex-biased miRNA Adult Melopsittacus undulatus |
url |
https://peerj.com/articles/4615.pdf |
work_keys_str_mv |
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