Establishment of a Challenge Model for Sheeppox Virus Infection
Sheeppox virus (SPPV) together with goatpox virus and lumpy skin disease virus form the genus <i>Capripoxvirus</i> of the <i>Poxviridae</i> family. Due to their great economic importance and major impact on livelihood of small-scale farmers, OIE guidelines classify capripox v...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2020-12-01
|
Series: | Microorganisms |
Subjects: | |
Online Access: | https://www.mdpi.com/2076-2607/8/12/2001 |
id |
doaj-aac118c0cb3748f5ab84cc2bd94f2b77 |
---|---|
record_format |
Article |
spelling |
doaj-aac118c0cb3748f5ab84cc2bd94f2b772020-12-16T00:02:23ZengMDPI AGMicroorganisms2076-26072020-12-0182001200110.3390/microorganisms8122001Establishment of a Challenge Model for Sheeppox Virus InfectionJanika Wolff0Sahar Abd El Rahman1Jacqueline King2Mohamed El-Beskawy3Anne Pohlmann4Martin Beer5Bernd Hoffmann6Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Südufer 10, Insel Riems, D-17493 Greifswald, GermanyDepartment of Virology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, EgyptInstitute of Diagnostic Virology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Südufer 10, Insel Riems, D-17493 Greifswald, GermanyDepartment of Animal Medicine, Faculty of Veterinary Medicine, Matrouh University, Matrouh 51744, EgyptInstitute of Diagnostic Virology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Südufer 10, Insel Riems, D-17493 Greifswald, GermanyInstitute of Diagnostic Virology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Südufer 10, Insel Riems, D-17493 Greifswald, GermanyInstitute of Diagnostic Virology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Südufer 10, Insel Riems, D-17493 Greifswald, GermanySheeppox virus (SPPV) together with goatpox virus and lumpy skin disease virus form the genus <i>Capripoxvirus</i> of the <i>Poxviridae</i> family. Due to their great economic importance and major impact on livelihood of small-scale farmers, OIE guidelines classify capripox viruses as notifiable diseases. In the present study, we examined pathogenesis of an Indian SPPV isolate and an Egyptian SPPV isolate in sheep. Three different infection routes were tested: (i) intravenous infection, (ii) intranasal infection and (iii) contact transmission between infected and naïve sheep. Clinical course, viremia and viral shedding as well as seroconversion were analyzed in order to establish a challenge model for SPPV infections that can be used in future vaccine studies. Next to in vivo characterization, both SPPV strains underwent next- and third-generation sequencing to obtain high quality full-length genomes for genetic characterization and comparison to already published SPPV sequences.https://www.mdpi.com/2076-2607/8/12/2001capripoxsheeppoxSPPVfield strainpathogenesischallenge model |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Janika Wolff Sahar Abd El Rahman Jacqueline King Mohamed El-Beskawy Anne Pohlmann Martin Beer Bernd Hoffmann |
spellingShingle |
Janika Wolff Sahar Abd El Rahman Jacqueline King Mohamed El-Beskawy Anne Pohlmann Martin Beer Bernd Hoffmann Establishment of a Challenge Model for Sheeppox Virus Infection Microorganisms capripox sheeppox SPPV field strain pathogenesis challenge model |
author_facet |
Janika Wolff Sahar Abd El Rahman Jacqueline King Mohamed El-Beskawy Anne Pohlmann Martin Beer Bernd Hoffmann |
author_sort |
Janika Wolff |
title |
Establishment of a Challenge Model for Sheeppox Virus Infection |
title_short |
Establishment of a Challenge Model for Sheeppox Virus Infection |
title_full |
Establishment of a Challenge Model for Sheeppox Virus Infection |
title_fullStr |
Establishment of a Challenge Model for Sheeppox Virus Infection |
title_full_unstemmed |
Establishment of a Challenge Model for Sheeppox Virus Infection |
title_sort |
establishment of a challenge model for sheeppox virus infection |
publisher |
MDPI AG |
series |
Microorganisms |
issn |
2076-2607 |
publishDate |
2020-12-01 |
description |
Sheeppox virus (SPPV) together with goatpox virus and lumpy skin disease virus form the genus <i>Capripoxvirus</i> of the <i>Poxviridae</i> family. Due to their great economic importance and major impact on livelihood of small-scale farmers, OIE guidelines classify capripox viruses as notifiable diseases. In the present study, we examined pathogenesis of an Indian SPPV isolate and an Egyptian SPPV isolate in sheep. Three different infection routes were tested: (i) intravenous infection, (ii) intranasal infection and (iii) contact transmission between infected and naïve sheep. Clinical course, viremia and viral shedding as well as seroconversion were analyzed in order to establish a challenge model for SPPV infections that can be used in future vaccine studies. Next to in vivo characterization, both SPPV strains underwent next- and third-generation sequencing to obtain high quality full-length genomes for genetic characterization and comparison to already published SPPV sequences. |
topic |
capripox sheeppox SPPV field strain pathogenesis challenge model |
url |
https://www.mdpi.com/2076-2607/8/12/2001 |
work_keys_str_mv |
AT janikawolff establishmentofachallengemodelforsheeppoxvirusinfection AT saharabdelrahman establishmentofachallengemodelforsheeppoxvirusinfection AT jacquelineking establishmentofachallengemodelforsheeppoxvirusinfection AT mohamedelbeskawy establishmentofachallengemodelforsheeppoxvirusinfection AT annepohlmann establishmentofachallengemodelforsheeppoxvirusinfection AT martinbeer establishmentofachallengemodelforsheeppoxvirusinfection AT berndhoffmann establishmentofachallengemodelforsheeppoxvirusinfection |
_version_ |
1724381896989736960 |