Neurodegeneration and Glial Response after Acute Striatal Stroke: Histological Basis for Neuroprotective Studies
Stroke is a leading cause of death and neurological disability worldwide and striatal ischemic stroke is frequent in humans due to obstruction of middle cerebral artery. Several pathological events underlie damage progression and a comprehensive description of the pathological features following exp...
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doaj-aac4a04caf53448b9bec21030a09e1842020-11-24T22:51:20ZengHindawi LimitedOxidative Medicine and Cellular Longevity1942-09001942-09942016-01-01201610.1155/2016/31735643173564Neurodegeneration and Glial Response after Acute Striatal Stroke: Histological Basis for Neuroprotective StudiesRafael R. Lima0Luana N. S. Santana1Rafael M. Fernandes2Elder M. Nascimento3Ana Carolina A. Oliveira4Luanna M. P. Fernandes5Enio Mauricio N. dos Santos6Patrycy Assis N. Tavares7Ijair Rogério dos Santos8Adriano Gimarães-Santos9Walace Gomes-Leal10Institute of Biological Sciences, Laboratory of Functional and Structural Biology, Federal University of Pará, 66075-900 Belém, PA, BrazilInstitute of Biological Sciences, Laboratory of Functional and Structural Biology, Federal University of Pará, 66075-900 Belém, PA, BrazilInstitute of Biological Sciences, Laboratory of Functional and Structural Biology, Federal University of Pará, 66075-900 Belém, PA, BrazilInstitute of Biological Sciences, Laboratory of Experimental Neuroprotection and Neuroregeneration, Federal University of Pará, 66075-900 Belém, PA, BrazilInstitute of Biological Sciences, Laboratory of Functional and Structural Biology, Federal University of Pará, 66075-900 Belém, PA, BrazilInstitute of Biological Sciences, Laboratory of Functional and Structural Biology, Federal University of Pará, 66075-900 Belém, PA, BrazilInstitute of Biological Sciences, Laboratory of Functional and Structural Biology, Federal University of Pará, 66075-900 Belém, PA, BrazilInstitute of Biological Sciences, Laboratory of Experimental Neuroprotection and Neuroregeneration, Federal University of Pará, 66075-900 Belém, PA, BrazilInstitute of Biological Sciences, Laboratory of Experimental Neuroprotection and Neuroregeneration, Federal University of Pará, 66075-900 Belém, PA, BrazilInstitute of Biological Sciences, Laboratory of Experimental Neuroprotection and Neuroregeneration, Federal University of Pará, 66075-900 Belém, PA, BrazilInstitute of Biological Sciences, Laboratory of Experimental Neuroprotection and Neuroregeneration, Federal University of Pará, 66075-900 Belém, PA, BrazilStroke is a leading cause of death and neurological disability worldwide and striatal ischemic stroke is frequent in humans due to obstruction of middle cerebral artery. Several pathological events underlie damage progression and a comprehensive description of the pathological features following experimental stroke in both acute and chronic survival times is a necessary step for further functional studies. Here, we explored the patterns of microglial activation, astrocytosis, oligodendrocyte damage, myelin impairment, and Nogo-A immunoreactivity between 3 and 30 postlesion days (PLDs) after experimental striatal stroke in adult rats induced by microinjections of endothelin-1 (ET-1). The focal ischemia induced tissue loss concomitant with intense microglia activation between 3 and 14 PLDs (maximum at 7 PLDs), decreasing afterward. Astrocytosis was maximum around 7 PLDs. Oligodendrocyte damage and Nogo-A upregulation were higher at 3 PLDs. Myelin impairment was maximum between 7 and 14 PLDs. Nogo-A expression was higher in the first week in comparison to control. The results add important histopathological features of ET-1 induced stroke in subacute and chronic survival times. In addition, the establishment of the temporal evolution of these neuropathological events is an important step for future studies seeking suitable neuroprotective drugs targeting neuroinflammation and white matter damage.http://dx.doi.org/10.1155/2016/3173564 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rafael R. Lima Luana N. S. Santana Rafael M. Fernandes Elder M. Nascimento Ana Carolina A. Oliveira Luanna M. P. Fernandes Enio Mauricio N. dos Santos Patrycy Assis N. Tavares Ijair Rogério dos Santos Adriano Gimarães-Santos Walace Gomes-Leal |
spellingShingle |
Rafael R. Lima Luana N. S. Santana Rafael M. Fernandes Elder M. Nascimento Ana Carolina A. Oliveira Luanna M. P. Fernandes Enio Mauricio N. dos Santos Patrycy Assis N. Tavares Ijair Rogério dos Santos Adriano Gimarães-Santos Walace Gomes-Leal Neurodegeneration and Glial Response after Acute Striatal Stroke: Histological Basis for Neuroprotective Studies Oxidative Medicine and Cellular Longevity |
author_facet |
Rafael R. Lima Luana N. S. Santana Rafael M. Fernandes Elder M. Nascimento Ana Carolina A. Oliveira Luanna M. P. Fernandes Enio Mauricio N. dos Santos Patrycy Assis N. Tavares Ijair Rogério dos Santos Adriano Gimarães-Santos Walace Gomes-Leal |
author_sort |
Rafael R. Lima |
title |
Neurodegeneration and Glial Response after Acute Striatal Stroke: Histological Basis for Neuroprotective Studies |
title_short |
Neurodegeneration and Glial Response after Acute Striatal Stroke: Histological Basis for Neuroprotective Studies |
title_full |
Neurodegeneration and Glial Response after Acute Striatal Stroke: Histological Basis for Neuroprotective Studies |
title_fullStr |
Neurodegeneration and Glial Response after Acute Striatal Stroke: Histological Basis for Neuroprotective Studies |
title_full_unstemmed |
Neurodegeneration and Glial Response after Acute Striatal Stroke: Histological Basis for Neuroprotective Studies |
title_sort |
neurodegeneration and glial response after acute striatal stroke: histological basis for neuroprotective studies |
publisher |
Hindawi Limited |
series |
Oxidative Medicine and Cellular Longevity |
issn |
1942-0900 1942-0994 |
publishDate |
2016-01-01 |
description |
Stroke is a leading cause of death and neurological disability worldwide and striatal ischemic stroke is frequent in humans due to obstruction of middle cerebral artery. Several pathological events underlie damage progression and a comprehensive description of the pathological features following experimental stroke in both acute and chronic survival times is a necessary step for further functional studies. Here, we explored the patterns of microglial activation, astrocytosis, oligodendrocyte damage, myelin impairment, and Nogo-A immunoreactivity between 3 and 30 postlesion days (PLDs) after experimental striatal stroke in adult rats induced by microinjections of endothelin-1 (ET-1). The focal ischemia induced tissue loss concomitant with intense microglia activation between 3 and 14 PLDs (maximum at 7 PLDs), decreasing afterward. Astrocytosis was maximum around 7 PLDs. Oligodendrocyte damage and Nogo-A upregulation were higher at 3 PLDs. Myelin impairment was maximum between 7 and 14 PLDs. Nogo-A expression was higher in the first week in comparison to control. The results add important histopathological features of ET-1 induced stroke in subacute and chronic survival times. In addition, the establishment of the temporal evolution of these neuropathological events is an important step for future studies seeking suitable neuroprotective drugs targeting neuroinflammation and white matter damage. |
url |
http://dx.doi.org/10.1155/2016/3173564 |
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