Genome Modification Technologies and Their Applications in Avian Species

• Main Authors: Hong Jo Lee, Young Min Kim, Tamao Ono, Jae Yong Han
• Format: Article
• Language: English
• Published: MDPI AG 2017-10-01
• Series: International Journal of Molecular Sciences
• Subjects: genome modification; avian; transgenic technology; programmable genome editing; CRISPR/Cas9; primordial germ cells
• Online Access: https://www.mdpi.com/1422-0067/18/11/2245

The rapid development of genome modification technology has provided many great benefits in diverse areas of research and industry. Genome modification technologies have also been actively used in a variety of research areas and fields of industry in avian species. Transgenic technologies such as lentiviral systems and piggyBac transposition have been used to produce transgenic birds for diverse purposes. In recent years, newly developed programmable genome editing tools such as transcription activator-like effector nuclease (TALEN) and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (CRISPR/Cas9) have also been successfully adopted in avian systems with primordial germ cell (PGC)-mediated genome modification. These genome modification technologies are expected to be applied to practical uses beyond system development itself. The technologies could be used to enhance economic traits in poultry such as acquiring a disease resistance or producing functional proteins in eggs. Furthermore, novel avian models of human diseases or embryonic development could also be established for research purposes. In this review, we discuss diverse genome modification technologies used in avian species, and future applications of avian biotechnology.