Analysis of two populations of the Uruguayan canine breed Cimarron (Canis familiaris) using rapd markers

Analysis of two populations of the Uruguayan canine breed Cimarron (Canis familiaris) using rapd markers

Objective. To analyze the genetic variability in two populations of the native canine breed “Uruguayan Cimarron” from different areas of the country. Materials and methods. DNA was isolated from 40 animals of the Cimarron breed. Using PCR, 11 RAPD (random amplified polymorphism of DNA) molecular mar...

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Bibliographic Details
Main Authors: Silvia Llambí D, Rosa Gagliardi B, Mónica Martínez T, Jorge Estevez C, Ana Gorozurreta O, María Victoria Arruga L
Format: Article
Language:English
Published: Universidad de Cordoba 2008-12-01
Series:Revista MVZ Cordoba
Subjects:
Canis familiaris
genome
Uruguay
Online Access:http://apps.unicordoba.edu.co/revistas/revistamvz/mvz-133/v13n3a4.pdf
Description
Summary:Objective. To analyze the genetic variability in two populations of the native canine breed “Uruguayan Cimarron” from different areas of the country. Materials and methods. DNA was isolated from 40 animals of the Cimarron breed. Using PCR, 11 RAPD (random amplified polymorphism of DNA) molecular markers were analyzed. Results. 14.9% of loci were polymorphic. We found an index of shared bands of 0.91, a genetic distance of 0.16 and a Nei’s average diversity index of 0.08. Conclusions. Both populations present a high homogeneity and genetic identity for these markers suggesting that they belong to a common genetic nucleus.
ISSN:0122-0268
1909-0544

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