Effects of 4 kinds of dental metal alloy extraction on expression of inflanmatory cytokine in the inflamed periodontal tissue

• Main Authors: WU Li-xia, ZHANG Li-li, WANG Yuan-qin, LI Lei, LIANG Shan-shan
• Format: Article
• Language: zho
• Published: Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases 2016-08-01
• Series: 口腔疾病防治
• Subjects: Gingival fibroblast cell; Co-Cr alloy; Ti alloy; Ti; Au-Pd alloy
• Online Access: http://www.kqjbfz.com/EN/10.12016/j.issn.2096-1456.2016.08.003

Objective To evaluate the inflammatory cytokine expressions of inflamed human gingival fibroblast cells (iHGFs) stimulated in 4 types of dental metal extractions.Methods Inflamed gingival tissues from 10 patients with periodontitis were collected, and primary iHGFs were cultured. The cells were stimulated by Co-Cr, Ti alloy, pure Ti and Au-Pd alloy extractions respectively, and DMEM was used as blank control. Protein productions of pro-inflammatory mediators and related mRNA expressions were measured with ELISA and RT-PCR. Statistics were tested with Wilcoxon matched-pairs test.Results ELISA and RT-PCR results showed the protein and mRNA expressions of interleukin-6, interleukin-1β, tumor necrosis factor-α in Ni-Cr and Ti alloy groups were both higher than the control group (z = 2.803, P = 0.005). However, there was no significant difference in pure Ti and Au-Pd groups compared with the control group (P > 0.05).Conclusion Pure Ti and Au-Pd alloy have a better biocompatibility than Co-Cr and Ti alloy in inflamed periodontal tissues.