Aedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-Wolbachia symbiosis
Abstract In the present work, we established two novel embryonic cell lines from the mosquito Aedes fluviatilis containing or not the naturally occurring symbiont bacteria Wolbachia, which were called wAflu1 and Aflu2, respectively. We also obtained wAflu1 without Wolbachia after tetracycline treatm...
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Format: | Article |
Language: | English |
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Nature Publishing Group
2021-09-01
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Series: | Scientific Reports |
Online Access: | https://doi.org/10.1038/s41598-021-98738-7 |
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doaj-aaf52a57e54445829414d706c350608d |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Christiano Calixto Conceição Jhenifer Nascimento da Silva Angélica Arcanjo Cíntia Lopes Nogueira Leonardo Araujo de Abreu Pedro Lagerblad de Oliveira Katia C. Gondim Bruno Moraes Stephanie Serafim de Carvalho Renato Martins da Silva Itabajara da Silva Vaz Luciano Andrade Moreira Carlos Logullo |
spellingShingle |
Christiano Calixto Conceição Jhenifer Nascimento da Silva Angélica Arcanjo Cíntia Lopes Nogueira Leonardo Araujo de Abreu Pedro Lagerblad de Oliveira Katia C. Gondim Bruno Moraes Stephanie Serafim de Carvalho Renato Martins da Silva Itabajara da Silva Vaz Luciano Andrade Moreira Carlos Logullo Aedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-Wolbachia symbiosis Scientific Reports |
author_facet |
Christiano Calixto Conceição Jhenifer Nascimento da Silva Angélica Arcanjo Cíntia Lopes Nogueira Leonardo Araujo de Abreu Pedro Lagerblad de Oliveira Katia C. Gondim Bruno Moraes Stephanie Serafim de Carvalho Renato Martins da Silva Itabajara da Silva Vaz Luciano Andrade Moreira Carlos Logullo |
author_sort |
Christiano Calixto Conceição |
title |
Aedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-Wolbachia symbiosis |
title_short |
Aedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-Wolbachia symbiosis |
title_full |
Aedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-Wolbachia symbiosis |
title_fullStr |
Aedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-Wolbachia symbiosis |
title_full_unstemmed |
Aedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-Wolbachia symbiosis |
title_sort |
aedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-wolbachia symbiosis |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2021-09-01 |
description |
Abstract In the present work, we established two novel embryonic cell lines from the mosquito Aedes fluviatilis containing or not the naturally occurring symbiont bacteria Wolbachia, which were called wAflu1 and Aflu2, respectively. We also obtained wAflu1 without Wolbachia after tetracycline treatment, named wAflu1.tet. Morphofunctional characterization was performed to help elucidate the symbiont-host interaction in the context of energy metabolism regulation and molecular mechanisms of the immune responses involved. The presence of Wolbachia pipientis improves energy performance in A. fluviatilis cells; it affects the regulation of key energy sources such as lipids, proteins, and carbohydrates, making the distribution of actin more peripheral and with extensions that come into contact with neighboring cells. Additionally, innate immunity mechanisms were activated, showing that the wAflu1 and wAflu1.tet cells are responsive after the stimulus using Gram negative bacteria. Therefore, this work confirms the natural, mutually co-regulating symbiotic relationship between W. pipientis and A. fluviatilis, modulating the host metabolism and immune pathway activation. The results presented here add important resources to the current knowledge of Wolbachia-arthropod interactions. |
url |
https://doi.org/10.1038/s41598-021-98738-7 |
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doaj-aaf52a57e54445829414d706c350608d2021-10-03T11:29:12ZengNature Publishing GroupScientific Reports2045-23222021-09-0111111210.1038/s41598-021-98738-7Aedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-Wolbachia symbiosisChristiano Calixto Conceição0Jhenifer Nascimento da Silva1Angélica Arcanjo2Cíntia Lopes Nogueira3Leonardo Araujo de Abreu4Pedro Lagerblad de Oliveira5Katia C. Gondim6Bruno Moraes7Stephanie Serafim de Carvalho8Renato Martins da Silva9Itabajara da Silva Vaz10Luciano Andrade Moreira11Carlos Logullo12Laboratório de Bioquímica de Artrópodes Hematófagos, Laboratório de Bioquímica e Fisiologia de Insetos – IBqM and Laboratório Integrado de Bioquímica Hatisaburo Masuda – NUPEM, Universidade Federal Do Rio de Janeiro - UFRJLaboratório de Bioquímica de Artrópodes Hematófagos, Laboratório de Bioquímica e Fisiologia de Insetos – IBqM and Laboratório Integrado de Bioquímica Hatisaburo Masuda – NUPEM, Universidade Federal Do Rio de Janeiro - UFRJLaboratório de Bioquímica de Artrópodes Hematófagos, Laboratório de Bioquímica e Fisiologia de Insetos – IBqM and Laboratório Integrado de Bioquímica Hatisaburo Masuda – NUPEM, Universidade Federal Do Rio de Janeiro - UFRJLaboratório de Bioquímica de Artrópodes Hematófagos, Laboratório de Bioquímica e Fisiologia de Insetos – IBqM and Laboratório Integrado de Bioquímica Hatisaburo Masuda – NUPEM, Universidade Federal Do Rio de Janeiro - UFRJLaboratório de Bioquímica de Artrópodes Hematófagos, Laboratório de Bioquímica e Fisiologia de Insetos – IBqM and Laboratório Integrado de Bioquímica Hatisaburo Masuda – NUPEM, Universidade Federal Do Rio de Janeiro - UFRJLaboratório de Bioquímica de Artrópodes Hematófagos, Laboratório de Bioquímica e Fisiologia de Insetos – IBqM and Laboratório Integrado de Bioquímica Hatisaburo Masuda – NUPEM, Universidade Federal Do Rio de Janeiro - UFRJLaboratório de Bioquímica de Artrópodes Hematófagos, Laboratório de Bioquímica e Fisiologia de Insetos – IBqM and Laboratório Integrado de Bioquímica Hatisaburo Masuda – NUPEM, Universidade Federal Do Rio de Janeiro - UFRJLaboratório de Bioquímica de Artrópodes Hematófagos, Laboratório de Bioquímica e Fisiologia de Insetos – IBqM and Laboratório Integrado de Bioquímica Hatisaburo Masuda – NUPEM, Universidade Federal Do Rio de Janeiro - UFRJLaboratório de Bioquímica de Artrópodes Hematófagos, Laboratório de Bioquímica e Fisiologia de Insetos – IBqM and Laboratório Integrado de Bioquímica Hatisaburo Masuda – NUPEM, Universidade Federal Do Rio de Janeiro - UFRJLaboratório de Bioquímica de Artrópodes Hematófagos, Laboratório de Bioquímica e Fisiologia de Insetos – IBqM and Laboratório Integrado de Bioquímica Hatisaburo Masuda – NUPEM, Universidade Federal Do Rio de Janeiro - UFRJCentro de Biotecnologia and Faculdade de Veterinária, Universidade Federal Do Rio Grande Do Sul - UFRGSInstituto Nacional de Ciência E Tecnologia Em Entomologia MolecularLaboratório de Bioquímica de Artrópodes Hematófagos, Laboratório de Bioquímica e Fisiologia de Insetos – IBqM and Laboratório Integrado de Bioquímica Hatisaburo Masuda – NUPEM, Universidade Federal Do Rio de Janeiro - UFRJAbstract In the present work, we established two novel embryonic cell lines from the mosquito Aedes fluviatilis containing or not the naturally occurring symbiont bacteria Wolbachia, which were called wAflu1 and Aflu2, respectively. We also obtained wAflu1 without Wolbachia after tetracycline treatment, named wAflu1.tet. Morphofunctional characterization was performed to help elucidate the symbiont-host interaction in the context of energy metabolism regulation and molecular mechanisms of the immune responses involved. The presence of Wolbachia pipientis improves energy performance in A. fluviatilis cells; it affects the regulation of key energy sources such as lipids, proteins, and carbohydrates, making the distribution of actin more peripheral and with extensions that come into contact with neighboring cells. Additionally, innate immunity mechanisms were activated, showing that the wAflu1 and wAflu1.tet cells are responsive after the stimulus using Gram negative bacteria. Therefore, this work confirms the natural, mutually co-regulating symbiotic relationship between W. pipientis and A. fluviatilis, modulating the host metabolism and immune pathway activation. The results presented here add important resources to the current knowledge of Wolbachia-arthropod interactions.https://doi.org/10.1038/s41598-021-98738-7 |