Variability at the 3′ untranslated region of the HLA-G gene: a study on patients with AIDS and cytomegalovirus retinochoroiditis
Abstract Cytomegalovirus retinochoroiditis (CMV-R) is the primary cause of blindness among AIDS patients. Since HLA-G is associated with the modulation of the immune response, we hypothesized that variability at the 3′ untraslated region (3′UTR) of the gene could be implicated on the predisposition...
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doaj-ab2e1992cc9441b18f9b07c8a415dd252020-12-08T10:03:23ZengNature Publishing GroupScientific Reports2045-23222020-10-011011710.1038/s41598-020-75639-9Variability at the 3′ untranslated region of the HLA-G gene: a study on patients with AIDS and cytomegalovirus retinochoroiditisLuciana de Morais Vicente0Erick C. Castelli1Maria de Lourdes Veronese Rodrigues2Neifi Hassan Saloum Deghaide3Celso Teixeira Mendes-Junior4João M. Furtado5Eduardo Antonio Donadi6Ophthalmology Division, Departament of Ophthalmology, Otorhinolaryngology and Head and Neck Surgery, Ribeirão Preto Medical School, University of São PauloPathology Department, School of Medicine, São Paulo State University (UNESP)Ophthalmology Division, Departament of Ophthalmology, Otorhinolaryngology and Head and Neck Surgery, Ribeirão Preto Medical School, University of São PauloOphthalmology Division, Departament of Ophthalmology, Otorhinolaryngology and Head and Neck Surgery, Ribeirão Preto Medical School, University of São PauloDepartamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São PauloOphthalmology Division, Departament of Ophthalmology, Otorhinolaryngology and Head and Neck Surgery, Ribeirão Preto Medical School, University of São PauloClinical Immunology Division, Department of Internal Medicine, Ribeirão Preto Medical School, University of São PauloAbstract Cytomegalovirus retinochoroiditis (CMV-R) is the primary cause of blindness among AIDS patients. Since HLA-G is associated with the modulation of the immune response, we hypothesized that variability at the 3′ untraslated region (3′UTR) of the gene could be implicated on the predisposition to CMV-R. We evaluated whether HLA-G 3′UTR influences CMV-R development in Brazilian AIDS patients. Peripheral blood DNA was obtained from two groups of patients: (1) AIDS exhibiting CMV-R (n = 40) and (2) AIDS without CMV-R (n = 147). HLA-G 3′UTR typing was performed using sequencing analysis. Allele, genotype and haplotype frequencies were evaluated using Fisher’s exact test accompanied by the calculation of the odds ratio and its 95% confidence interval (95% CI). The etiologic (EF) and preventive fractions were also estimated. Compared to AIDS patients without CMV-R, AIDS patients with CMV-R showed increased frequencies of the: (1) + 3001T allele, (2) the + 3001C/T genotype and (3) the UTR-17 (InsTTCCGTGACG) haplotype (EFs = 0.02–0.04). The UTR-3 (DelTCCGCGACG) haplotype was associated with protection against CMV-R development. Although the risk for developing CMR-V at the population level was relatively low (EF), the identification of HLA-G 3′UTR variation sites may help to further evaluate the role of post-transcriptional factors that may contribute to the existent immunosuppresion caused by HIV per se.https://doi.org/10.1038/s41598-020-75639-9 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Luciana de Morais Vicente Erick C. Castelli Maria de Lourdes Veronese Rodrigues Neifi Hassan Saloum Deghaide Celso Teixeira Mendes-Junior João M. Furtado Eduardo Antonio Donadi |
spellingShingle |
Luciana de Morais Vicente Erick C. Castelli Maria de Lourdes Veronese Rodrigues Neifi Hassan Saloum Deghaide Celso Teixeira Mendes-Junior João M. Furtado Eduardo Antonio Donadi Variability at the 3′ untranslated region of the HLA-G gene: a study on patients with AIDS and cytomegalovirus retinochoroiditis Scientific Reports |
author_facet |
Luciana de Morais Vicente Erick C. Castelli Maria de Lourdes Veronese Rodrigues Neifi Hassan Saloum Deghaide Celso Teixeira Mendes-Junior João M. Furtado Eduardo Antonio Donadi |
author_sort |
Luciana de Morais Vicente |
title |
Variability at the 3′ untranslated region of the HLA-G gene: a study on patients with AIDS and cytomegalovirus retinochoroiditis |
title_short |
Variability at the 3′ untranslated region of the HLA-G gene: a study on patients with AIDS and cytomegalovirus retinochoroiditis |
title_full |
Variability at the 3′ untranslated region of the HLA-G gene: a study on patients with AIDS and cytomegalovirus retinochoroiditis |
title_fullStr |
Variability at the 3′ untranslated region of the HLA-G gene: a study on patients with AIDS and cytomegalovirus retinochoroiditis |
title_full_unstemmed |
Variability at the 3′ untranslated region of the HLA-G gene: a study on patients with AIDS and cytomegalovirus retinochoroiditis |
title_sort |
variability at the 3′ untranslated region of the hla-g gene: a study on patients with aids and cytomegalovirus retinochoroiditis |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2020-10-01 |
description |
Abstract Cytomegalovirus retinochoroiditis (CMV-R) is the primary cause of blindness among AIDS patients. Since HLA-G is associated with the modulation of the immune response, we hypothesized that variability at the 3′ untraslated region (3′UTR) of the gene could be implicated on the predisposition to CMV-R. We evaluated whether HLA-G 3′UTR influences CMV-R development in Brazilian AIDS patients. Peripheral blood DNA was obtained from two groups of patients: (1) AIDS exhibiting CMV-R (n = 40) and (2) AIDS without CMV-R (n = 147). HLA-G 3′UTR typing was performed using sequencing analysis. Allele, genotype and haplotype frequencies were evaluated using Fisher’s exact test accompanied by the calculation of the odds ratio and its 95% confidence interval (95% CI). The etiologic (EF) and preventive fractions were also estimated. Compared to AIDS patients without CMV-R, AIDS patients with CMV-R showed increased frequencies of the: (1) + 3001T allele, (2) the + 3001C/T genotype and (3) the UTR-17 (InsTTCCGTGACG) haplotype (EFs = 0.02–0.04). The UTR-3 (DelTCCGCGACG) haplotype was associated with protection against CMV-R development. Although the risk for developing CMR-V at the population level was relatively low (EF), the identification of HLA-G 3′UTR variation sites may help to further evaluate the role of post-transcriptional factors that may contribute to the existent immunosuppresion caused by HIV per se. |
url |
https://doi.org/10.1038/s41598-020-75639-9 |
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