Optimization of Extraction Parameters of Bioactive Molecules from Salvia officinalis Using Experimental Design
Description of the subject: Salvia officinalis is a medicinal plant traditionally used for the treatment of several diseases such as diabetes and inflammatory diseases, until now, many studies have been conducted on its chemical composition and its biological activities, but optimization of extrac...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Phytochemistry & Organic Synthesis Laboratory
2017-11-01
|
Series: | PhytoChem & BioSub Journal |
Online Access: | https://drive.google.com/file/d/1dfRF3IZe5Fjr7kFbFDmDbboAfuG3HjjR/view?usp=sharing |
Summary: | Description of the subject: Salvia officinalis is a medicinal plant traditionally used for the treatment
of several diseases such as diabetes and inflammatory diseases, until now, many studies have been
conducted on its chemical composition and its biological activities, but optimization of extraction
methods of its chemical compounds don’t exist in the bibliography.
Objectives: The aim of this work is to study and optimize the extraction parameters of bioactive
molecules from sage leaves.
Methods: The extraction of bioactive molecules from the plant was carried out by soxhlet extractor
using 3 different solvents (ethanol, dichloromethane and hexane), we studied the extraction process
for each solvent using a full factorial experimental design with two factors and two levels. The
selected factors are ‘extraction time’ and ‘heating power’; the studied answers are ‘extraction yield’
and ‘polyphenols yield’.
Results: After the exploitation of results, we determined the effects of factors on the responses and
developed the mathematical models of processes. Generally, the effect of heating power on the
extraction yield is more important than the extraction time. Ethanol’s use allows the obtention of
better results compared with hexane and dichloromethane.
Conclusions: Ethanol is the reference organic solvent for the extraction of bioactive molecules from
Salvia officinalis leaves, the increase in heating power reduces extraction time and increases extraction
yield.
|
---|---|
ISSN: | 2170-1768 2170-1768 |