A Molecular Approach Applied to Enteroviruses Surveillance in Northern Taiwan, 2008-2012.
Traditional methods for detection and serotyping of enterovirus infections are virus isolation and immunofluorescence assay (VI-IFA), which are labor-intensive and time-consuming. Recently, VP1 gene has been targeted to develop a CODEHOP-based RT-PCR (VP1-CODEHOP) for the same purpose. In this study...
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doaj-ab5f2c5084644d29b7cbba2a2a1912a12020-11-24T22:21:33ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-011112e016753210.1371/journal.pone.0167532A Molecular Approach Applied to Enteroviruses Surveillance in Northern Taiwan, 2008-2012.Wan-Yu ChungPai-Shan ChiangShu-Ting LuoTzou-Yien LinKuo-Chien TsaoMin-Shi LeeTraditional methods for detection and serotyping of enterovirus infections are virus isolation and immunofluorescence assay (VI-IFA), which are labor-intensive and time-consuming. Recently, VP1 gene has been targeted to develop a CODEHOP-based RT-PCR (VP1-CODEHOP) for the same purpose. In this study, we conducted a 5-year enterovirus surveillance comparing the VI-IFA and VP1-CODEHOP tests. Throat swabs were collected from 431 pediatric patients and 208(48%) and 250(58%) were tested positive by the VI-IFA and VP1-CODEHOP tests, respectively. Among the 47 cases who had inconsistent results between the VI-IFA and VP1-CODEHOP tests and provided paired sera for serological verifications, correct diagnosis for the VI-IFA and VP1-CODEHOP were 5(11%) and 40(85%) cases, respectively. Therefore, the VP1-CODEHOP is more reliable for detection of human enteroviruses than the VI-IFA. Based on serological verifications for the eight cases who had inconsistent serotypes between the two tests and provided paired sera, five and two showed consistent serotypes with the VP1-CODEHOP and VI-IFA tests, respectively. CVA16, CVA6 and EV71 were the most prevalent serotypes in northern Taiwan, 2008~2012. Moreover, variant CVA2, CVA6 and EV71 viruses were further identified based on phylogenetic analysis of partial VP1 sequences. In conclusion, the VP1-CODEHOP test could be used as the primary method for enterovirus surveillance to support decision-making for outbreak control.http://europepmc.org/articles/PMC5131993?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wan-Yu Chung Pai-Shan Chiang Shu-Ting Luo Tzou-Yien Lin Kuo-Chien Tsao Min-Shi Lee |
spellingShingle |
Wan-Yu Chung Pai-Shan Chiang Shu-Ting Luo Tzou-Yien Lin Kuo-Chien Tsao Min-Shi Lee A Molecular Approach Applied to Enteroviruses Surveillance in Northern Taiwan, 2008-2012. PLoS ONE |
author_facet |
Wan-Yu Chung Pai-Shan Chiang Shu-Ting Luo Tzou-Yien Lin Kuo-Chien Tsao Min-Shi Lee |
author_sort |
Wan-Yu Chung |
title |
A Molecular Approach Applied to Enteroviruses Surveillance in Northern Taiwan, 2008-2012. |
title_short |
A Molecular Approach Applied to Enteroviruses Surveillance in Northern Taiwan, 2008-2012. |
title_full |
A Molecular Approach Applied to Enteroviruses Surveillance in Northern Taiwan, 2008-2012. |
title_fullStr |
A Molecular Approach Applied to Enteroviruses Surveillance in Northern Taiwan, 2008-2012. |
title_full_unstemmed |
A Molecular Approach Applied to Enteroviruses Surveillance in Northern Taiwan, 2008-2012. |
title_sort |
molecular approach applied to enteroviruses surveillance in northern taiwan, 2008-2012. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2016-01-01 |
description |
Traditional methods for detection and serotyping of enterovirus infections are virus isolation and immunofluorescence assay (VI-IFA), which are labor-intensive and time-consuming. Recently, VP1 gene has been targeted to develop a CODEHOP-based RT-PCR (VP1-CODEHOP) for the same purpose. In this study, we conducted a 5-year enterovirus surveillance comparing the VI-IFA and VP1-CODEHOP tests. Throat swabs were collected from 431 pediatric patients and 208(48%) and 250(58%) were tested positive by the VI-IFA and VP1-CODEHOP tests, respectively. Among the 47 cases who had inconsistent results between the VI-IFA and VP1-CODEHOP tests and provided paired sera for serological verifications, correct diagnosis for the VI-IFA and VP1-CODEHOP were 5(11%) and 40(85%) cases, respectively. Therefore, the VP1-CODEHOP is more reliable for detection of human enteroviruses than the VI-IFA. Based on serological verifications for the eight cases who had inconsistent serotypes between the two tests and provided paired sera, five and two showed consistent serotypes with the VP1-CODEHOP and VI-IFA tests, respectively. CVA16, CVA6 and EV71 were the most prevalent serotypes in northern Taiwan, 2008~2012. Moreover, variant CVA2, CVA6 and EV71 viruses were further identified based on phylogenetic analysis of partial VP1 sequences. In conclusion, the VP1-CODEHOP test could be used as the primary method for enterovirus surveillance to support decision-making for outbreak control. |
url |
http://europepmc.org/articles/PMC5131993?pdf=render |
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