Metalloproteins and apolipoprotein C: candidate plasma biomarkers of T2DM screened by comparative proteomics and lipidomics in ZDF rats

Abstract Background Early diagnosis of type 2 diabetes mellitus (T2DM) is still difficult. Screening of plasma biomarkers has great significance of optimizing diagnosis and predicting the complications of T2DM. Methods We used a special diet, Purina #5008, to induce diabetes in Zucker leptin recepto...

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Main Authors: Shuai Wang, Zhiyuan Lu, Yuxin Wang, Tianran Zhang, Xiaodong He
Format: Article
Language:English
Published: BMC 2020-08-01
Series:Nutrition & Metabolism
Subjects:
Online Access:http://link.springer.com/article/10.1186/s12986-020-00488-2
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spelling doaj-ab6f9d11091c43f18bbff4c8e5be48952020-11-25T03:26:26ZengBMCNutrition & Metabolism1743-70752020-08-0117111010.1186/s12986-020-00488-2Metalloproteins and apolipoprotein C: candidate plasma biomarkers of T2DM screened by comparative proteomics and lipidomics in ZDF ratsShuai Wang0Zhiyuan Lu1Yuxin Wang2Tianran Zhang3Xiaodong He4Institute of Toxicology, School of Public Health, Cheeloo College of Medicine, Shandong UniversityKey Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong UniversityDepartment of Physical and Chemical Inspection, School of Public Health, Cheeloo College of Medicine, Shandong UniversityDepartment of Physical and Chemical Inspection, School of Public Health, Cheeloo College of Medicine, Shandong UniversityDepartment of Physical and Chemical Inspection, School of Public Health, Cheeloo College of Medicine, Shandong UniversityAbstract Background Early diagnosis of type 2 diabetes mellitus (T2DM) is still difficult. Screening of plasma biomarkers has great significance of optimizing diagnosis and predicting the complications of T2DM. Methods We used a special diet, Purina #5008, to induce diabetes in Zucker leptin receptor gene-deficient rats (fa/fa) to establish Zucker diabetic fatty (ZDF) rats, simulating the early stage of T2DM. The differentially expressed proteins (DEP) and lipids (DEL), as potential biomarkers, were screened to compare the plasma expression levels in ZDF rats and their basic diet-fed wild-type controls (fa/+) by Tandem Mass Tags (TMT) and liquid chromatography-tandem mass spectrometry. Results These two groups had different plasma proteins and lipids profiles consisting of 84 DEPs and, 179 DELs identified in the positive ion mode and 178 DELs in the negative ion mode, respectively. Enrichment analysis of these different indicators showed that oxidative stress, insulin resistance and metabolic disorders of glycan and lipid played an important role in generating the difference. Some markers can be used as candidate biomarkers in prediction and treatments of T2DM, such as ceruloplasmin, apolipoprotein C-I, apolipoprotein C-II and apolipoprotein C-IV. Conclusion These plasma differences help to optimize the diagnosis and predict the complications of T2DM, although this remains to be verified in the crowd. Trace elements related-metalloproteins, such as ceruloplasmin, and lipid metabolism and transport-related apolipoprotein C are expected to be candidate biomarkers of T2DM and should be given more attention.http://link.springer.com/article/10.1186/s12986-020-00488-2Type 2 diabetes mellitusZucker diabetic fatty ratsProteomicsLipidomicsBiomarkers
collection DOAJ
language English
format Article
sources DOAJ
author Shuai Wang
Zhiyuan Lu
Yuxin Wang
Tianran Zhang
Xiaodong He
spellingShingle Shuai Wang
Zhiyuan Lu
Yuxin Wang
Tianran Zhang
Xiaodong He
Metalloproteins and apolipoprotein C: candidate plasma biomarkers of T2DM screened by comparative proteomics and lipidomics in ZDF rats
Nutrition & Metabolism
Type 2 diabetes mellitus
Zucker diabetic fatty rats
Proteomics
Lipidomics
Biomarkers
author_facet Shuai Wang
Zhiyuan Lu
Yuxin Wang
Tianran Zhang
Xiaodong He
author_sort Shuai Wang
title Metalloproteins and apolipoprotein C: candidate plasma biomarkers of T2DM screened by comparative proteomics and lipidomics in ZDF rats
title_short Metalloproteins and apolipoprotein C: candidate plasma biomarkers of T2DM screened by comparative proteomics and lipidomics in ZDF rats
title_full Metalloproteins and apolipoprotein C: candidate plasma biomarkers of T2DM screened by comparative proteomics and lipidomics in ZDF rats
title_fullStr Metalloproteins and apolipoprotein C: candidate plasma biomarkers of T2DM screened by comparative proteomics and lipidomics in ZDF rats
title_full_unstemmed Metalloproteins and apolipoprotein C: candidate plasma biomarkers of T2DM screened by comparative proteomics and lipidomics in ZDF rats
title_sort metalloproteins and apolipoprotein c: candidate plasma biomarkers of t2dm screened by comparative proteomics and lipidomics in zdf rats
publisher BMC
series Nutrition & Metabolism
issn 1743-7075
publishDate 2020-08-01
description Abstract Background Early diagnosis of type 2 diabetes mellitus (T2DM) is still difficult. Screening of plasma biomarkers has great significance of optimizing diagnosis and predicting the complications of T2DM. Methods We used a special diet, Purina #5008, to induce diabetes in Zucker leptin receptor gene-deficient rats (fa/fa) to establish Zucker diabetic fatty (ZDF) rats, simulating the early stage of T2DM. The differentially expressed proteins (DEP) and lipids (DEL), as potential biomarkers, were screened to compare the plasma expression levels in ZDF rats and their basic diet-fed wild-type controls (fa/+) by Tandem Mass Tags (TMT) and liquid chromatography-tandem mass spectrometry. Results These two groups had different plasma proteins and lipids profiles consisting of 84 DEPs and, 179 DELs identified in the positive ion mode and 178 DELs in the negative ion mode, respectively. Enrichment analysis of these different indicators showed that oxidative stress, insulin resistance and metabolic disorders of glycan and lipid played an important role in generating the difference. Some markers can be used as candidate biomarkers in prediction and treatments of T2DM, such as ceruloplasmin, apolipoprotein C-I, apolipoprotein C-II and apolipoprotein C-IV. Conclusion These plasma differences help to optimize the diagnosis and predict the complications of T2DM, although this remains to be verified in the crowd. Trace elements related-metalloproteins, such as ceruloplasmin, and lipid metabolism and transport-related apolipoprotein C are expected to be candidate biomarkers of T2DM and should be given more attention.
topic Type 2 diabetes mellitus
Zucker diabetic fatty rats
Proteomics
Lipidomics
Biomarkers
url http://link.springer.com/article/10.1186/s12986-020-00488-2
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