CircVAMP3: A circRNA with a Role in Alveolar Rhabdomyosarcoma Cell Cycle Progression

Circular RNAs (circRNAs), a class of covalently closed RNAs formed by a back-splicing reaction, have been involved in the regulation of diverse oncogenic processes. In this article we describe circVAMP3, a novel circular RNA overexpressed in RH4, a representative cell line of alveolar rhabdomyosarco...

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Main Authors:	Francesca Rossi, Alvaro Centrón-Broco, Dario Dattilo, Gaia Di Timoteo, Marco Guarnacci, Alessio Colantoni, Manuel Beltran Nebot, Irene Bozzoni
Format:	Article
Language:	English
Published:	MDPI AG 2021-06-01
Series:	Genes
Subjects:	circVAMP3 AKT m<sup>6</sup>A alveolar rhabdomyosarcoma cell cycle progression
Online Access:	https://www.mdpi.com/2073-4425/12/7/985

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spelling	doaj-ab706d18d1034f689915ca7f1d13a8d02021-07-23T13:41:42ZengMDPI AGGenes2073-44252021-06-011298598510.3390/genes12070985CircVAMP3: A circRNA with a Role in Alveolar Rhabdomyosarcoma Cell Cycle ProgressionFrancesca Rossi0Alvaro Centrón-Broco1Dario Dattilo2Gaia Di Timoteo3Marco Guarnacci4Alessio Colantoni5Manuel Beltran Nebot6Irene Bozzoni7Department of Biology and Biotechnology Charles Darwin, Sapienza University of Rome, 00185 Rome, ItalyDepartment of Biology and Biotechnology Charles Darwin, Sapienza University of Rome, 00185 Rome, ItalyDepartment of Biology and Biotechnology Charles Darwin, Sapienza University of Rome, 00185 Rome, ItalyDepartment of Biology and Biotechnology Charles Darwin, Sapienza University of Rome, 00185 Rome, ItalyDepartment of Biology and Biotechnology Charles Darwin, Sapienza University of Rome, 00185 Rome, ItalyCenter for Life Nano- & Neuro-Science, Fondazione Istituto Italiano di Tecnologia (IIT), 00161 Rome, ItalyDepartment of Biology and Biotechnology Charles Darwin, Sapienza University of Rome, 00185 Rome, ItalyDepartment of Biology and Biotechnology Charles Darwin, Sapienza University of Rome, 00185 Rome, ItalyCircular RNAs (circRNAs), a class of covalently closed RNAs formed by a back-splicing reaction, have been involved in the regulation of diverse oncogenic processes. In this article we describe circVAMP3, a novel circular RNA overexpressed in RH4, a representative cell line of alveolar rhabdomyosarcoma. We demonstrated that circVAMP3 has a differential m<sup>6</sup>A pattern opposed to its linear counterpart, suggesting that the two isoforms can be differently regulated by such RNA modification. Moreover, we show how circVAMP3 depletion in alveolar rhabdomyosarcoma cells can impair cell cycle progression, through the alteration of the AKT-related pathways, pointing to this non-coding RNA as a novel regulator of the alveolar rhabdomyosarcoma progression and as a putative future therapeutic target.https://www.mdpi.com/2073-4425/12/7/985circVAMP3AKTm<sup>6</sup>Aalveolar rhabdomyosarcomacell cycle progression
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Francesca Rossi Alvaro Centrón-Broco Dario Dattilo Gaia Di Timoteo Marco Guarnacci Alessio Colantoni Manuel Beltran Nebot Irene Bozzoni
spellingShingle	Francesca Rossi Alvaro Centrón-Broco Dario Dattilo Gaia Di Timoteo Marco Guarnacci Alessio Colantoni Manuel Beltran Nebot Irene Bozzoni CircVAMP3: A circRNA with a Role in Alveolar Rhabdomyosarcoma Cell Cycle Progression Genes circVAMP3 AKT m<sup>6</sup>A alveolar rhabdomyosarcoma cell cycle progression
author_facet	Francesca Rossi Alvaro Centrón-Broco Dario Dattilo Gaia Di Timoteo Marco Guarnacci Alessio Colantoni Manuel Beltran Nebot Irene Bozzoni
author_sort	Francesca Rossi
title	CircVAMP3: A circRNA with a Role in Alveolar Rhabdomyosarcoma Cell Cycle Progression
title_short	CircVAMP3: A circRNA with a Role in Alveolar Rhabdomyosarcoma Cell Cycle Progression
title_full	CircVAMP3: A circRNA with a Role in Alveolar Rhabdomyosarcoma Cell Cycle Progression
title_fullStr	CircVAMP3: A circRNA with a Role in Alveolar Rhabdomyosarcoma Cell Cycle Progression
title_full_unstemmed	CircVAMP3: A circRNA with a Role in Alveolar Rhabdomyosarcoma Cell Cycle Progression
title_sort	circvamp3: a circrna with a role in alveolar rhabdomyosarcoma cell cycle progression
publisher	MDPI AG
series	Genes
issn	2073-4425
publishDate	2021-06-01
description	Circular RNAs (circRNAs), a class of covalently closed RNAs formed by a back-splicing reaction, have been involved in the regulation of diverse oncogenic processes. In this article we describe circVAMP3, a novel circular RNA overexpressed in RH4, a representative cell line of alveolar rhabdomyosarcoma. We demonstrated that circVAMP3 has a differential m<sup>6</sup>A pattern opposed to its linear counterpart, suggesting that the two isoforms can be differently regulated by such RNA modification. Moreover, we show how circVAMP3 depletion in alveolar rhabdomyosarcoma cells can impair cell cycle progression, through the alteration of the AKT-related pathways, pointing to this non-coding RNA as a novel regulator of the alveolar rhabdomyosarcoma progression and as a putative future therapeutic target.
topic	circVAMP3 AKT m<sup>6</sup>A alveolar rhabdomyosarcoma cell cycle progression
url	https://www.mdpi.com/2073-4425/12/7/985
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CircVAMP3: A circRNA with a Role in Alveolar Rhabdomyosarcoma Cell Cycle Progression

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