Structure-based analysis of CysZ-mediated cellular uptake of sulfate
Sulfur, most abundantly found in the environment as sulfate (SO42-), is an essential element in metabolites required by all living cells, including amino acids, co-factors and vitamins. However, current understanding of the cellular delivery of SO42- at the molecular level is limited. CysZ has been...
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doaj-ab715dc2d4de43f39a8bdcbb5e3341272021-05-05T15:52:17ZengeLife Sciences Publications LtdeLife2050-084X2018-05-01710.7554/eLife.27829Structure-based analysis of CysZ-mediated cellular uptake of sulfateZahra Assur Sanghai0Qun Liu1Oliver B Clarke2https://orcid.org/0000-0003-1876-196XMeagan Belcher-Dufrisne3Pattama Wiriyasermkul4M Hunter Giese5Edgar Leal-Pinto6Brian Kloss7Shantelle Tabuso8James Love9Marco Punta10Surajit Banerjee11Kanagalaghatta R Rajashankar12Burkhard Rost13Diomedes Logothetis14Matthias Quick15Wayne A Hendrickson16Filippo Mancia17https://orcid.org/0000-0003-3293-2200Department of Physiology and Cellular Biophysics, Columbia University, New York, United States; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, United StatesBiology Department, Brookhaven National Laboratory, Upton, United StatesDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, United StatesDepartment of Physiology and Cellular Biophysics, Columbia University, New York, United StatesCenter for Molecular Recognition, Department of Psychiatry, Columbia University, New York, United StatesDepartment of Physiology and Cellular Biophysics, Columbia University, New York, United States; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, United StatesDepartment of Physiology and Biophysics, Virginia Commonwealth University School of Medicine, Richmond, United States; Department of Pharmaceutical Sciences, School of Pharmacy, Bouvé College of Health Sciences, Northeastern University, Boston, United StatesNew York Structural Biology Center, New York, United StatesNew York Structural Biology Center, New York, United StatesNew York Structural Biology Center, New York, United StatesCentre for Evolution and Cancer, The Institute of Cancer Research, London, United KingdomDepartment of Chemistry and Chemical Biology, Cornell University, NE-CAT, Argonne, United StatesDepartment of Chemistry and Chemical Biology, Cornell University, NE-CAT, Argonne, United StatesDepartment of Informatics, Technical University of Munich, Munich, GermanyDepartment of Physiology and Biophysics, Virginia Commonwealth University School of Medicine, Richmond, United States; Department of Pharmaceutical Sciences, School of Pharmacy, Bouvé College of Health Sciences, Northeastern University, Boston, United StatesCenter for Molecular Recognition, Department of Psychiatry, Columbia University, New York, United States; Division of Molecular Therapeutics, New York State Psychiatric Institute, New York, United StatesDepartment of Physiology and Cellular Biophysics, Columbia University, New York, United States; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, United States; New York Structural Biology Center, New York, United StatesDepartment of Physiology and Cellular Biophysics, Columbia University, New York, United StatesSulfur, most abundantly found in the environment as sulfate (SO42-), is an essential element in metabolites required by all living cells, including amino acids, co-factors and vitamins. However, current understanding of the cellular delivery of SO42- at the molecular level is limited. CysZ has been described as a SO42- permease, but its sequence family is without known structural precedent. Based on crystallographic structure information, SO42- binding and flux experiments, we provide insight into the molecular mechanism of CysZ-mediated translocation of SO42- across membranes. CysZ structures from three different bacterial species display a hitherto unknown fold and have subunits organized with inverted transmembrane topology. CysZ from Pseudomonas denitrificans assembles as a trimer of antiparallel dimers and the CysZ structures from two other species recapitulate dimers from this assembly. Mutational studies highlight the functional relevance of conserved CysZ residues.https://elifesciences.org/articles/27829Pseudomonas denitrificansPseudomonas fragiIdiomarina loihiensis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zahra Assur Sanghai Qun Liu Oliver B Clarke Meagan Belcher-Dufrisne Pattama Wiriyasermkul M Hunter Giese Edgar Leal-Pinto Brian Kloss Shantelle Tabuso James Love Marco Punta Surajit Banerjee Kanagalaghatta R Rajashankar Burkhard Rost Diomedes Logothetis Matthias Quick Wayne A Hendrickson Filippo Mancia |
spellingShingle |
Zahra Assur Sanghai Qun Liu Oliver B Clarke Meagan Belcher-Dufrisne Pattama Wiriyasermkul M Hunter Giese Edgar Leal-Pinto Brian Kloss Shantelle Tabuso James Love Marco Punta Surajit Banerjee Kanagalaghatta R Rajashankar Burkhard Rost Diomedes Logothetis Matthias Quick Wayne A Hendrickson Filippo Mancia Structure-based analysis of CysZ-mediated cellular uptake of sulfate eLife Pseudomonas denitrificans Pseudomonas fragi Idiomarina loihiensis |
author_facet |
Zahra Assur Sanghai Qun Liu Oliver B Clarke Meagan Belcher-Dufrisne Pattama Wiriyasermkul M Hunter Giese Edgar Leal-Pinto Brian Kloss Shantelle Tabuso James Love Marco Punta Surajit Banerjee Kanagalaghatta R Rajashankar Burkhard Rost Diomedes Logothetis Matthias Quick Wayne A Hendrickson Filippo Mancia |
author_sort |
Zahra Assur Sanghai |
title |
Structure-based analysis of CysZ-mediated cellular uptake of sulfate |
title_short |
Structure-based analysis of CysZ-mediated cellular uptake of sulfate |
title_full |
Structure-based analysis of CysZ-mediated cellular uptake of sulfate |
title_fullStr |
Structure-based analysis of CysZ-mediated cellular uptake of sulfate |
title_full_unstemmed |
Structure-based analysis of CysZ-mediated cellular uptake of sulfate |
title_sort |
structure-based analysis of cysz-mediated cellular uptake of sulfate |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2018-05-01 |
description |
Sulfur, most abundantly found in the environment as sulfate (SO42-), is an essential element in metabolites required by all living cells, including amino acids, co-factors and vitamins. However, current understanding of the cellular delivery of SO42- at the molecular level is limited. CysZ has been described as a SO42- permease, but its sequence family is without known structural precedent. Based on crystallographic structure information, SO42- binding and flux experiments, we provide insight into the molecular mechanism of CysZ-mediated translocation of SO42- across membranes. CysZ structures from three different bacterial species display a hitherto unknown fold and have subunits organized with inverted transmembrane topology. CysZ from Pseudomonas denitrificans assembles as a trimer of antiparallel dimers and the CysZ structures from two other species recapitulate dimers from this assembly. Mutational studies highlight the functional relevance of conserved CysZ residues. |
topic |
Pseudomonas denitrificans Pseudomonas fragi Idiomarina loihiensis |
url |
https://elifesciences.org/articles/27829 |
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