Urinary miRNAs as biomarkers for idiopathic osteonecrosis of femoral head: A multicentre study
Objectives: Urinary microRNAs (miRNAs) have shown great diagnostic and prognostic values for multiple diseases. The profile of urinary miRNAs in patients with idiopathic osteonecrosis of femoral head (ONFH) is currently unclear. Methods: We first randomly chose ten patients with each Association Res...
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doaj-ab8453a0fdd04d97951d267565474dfe2020-12-25T05:09:20ZengElsevierJournal of Orthopaedic Translation2214-031X2021-01-01265459Urinary miRNAs as biomarkers for idiopathic osteonecrosis of femoral head: A multicentre studyYongheng Ye0Yue Peng1Peiheng He2Qinqin Zhang3Dongliang Xu4Department of Joint Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong Province, 510080, ChinaDepartment of Otorhinolaryngology Head and Neck Surgery, Zhuhai People's Hospital, Zhuhai, Guangdong Province, 519000, ChinaDepartment of Joint Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong Province, 510080, ChinaDepartment of Thyroid and Breast Surgery, Nanxishan Hospital of Guangxi Zhuang Autonomous Region, Guilin, 541002, China; Corresponding author. Department of Thyroid and Breast Surgery, Nanxishan Hospital of Guangxi Zhuang Autonomous Region, Guilin, 541002, China.Department of Joint Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong Province, 510080, China; Corresponding author. Department of Joint Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong Province, 510080, China.Objectives: Urinary microRNAs (miRNAs) have shown great diagnostic and prognostic values for multiple diseases. The profile of urinary miRNAs in patients with idiopathic osteonecrosis of femoral head (ONFH) is currently unclear. Methods: We first randomly chose ten patients with each Association Research Circulation Osseous (ARCO) stage (I, II, III and IV) and ten healthy participants from the entire cohorts for initial screening. The miRNA polymerase chain reaction (PCR) array was then performed to identify the differentially abundant miRNAs in urine of these participants. We then verified the findings in the entire cohort. Clinical features including age, gender, bone mass index (BMI), lesion size and stages were recorded. We then analysed the association between the level of urinary miRNAs and clinical features. Results: Our data indicated that there were 13 differentially abundant miRNAs among all groups. Urinary miR-150 demonstrated the highest diagnostic value among all candidates. Urinary miR-185 and miR-133a increased by ARCO staging. The levels of urinary miR-4824 abruptly decreased after femoral head collapse (ARCO stage III and IV). Urinary miR-144 was the only marker that correlated with lesion size. Conclusions: The levels of urinary miRNAs are valuable biomarkers for idiopathic ONFH. Given the noninvasive nature of this test, it is potentially useful for diagnosis and monitoring of idiopathic ONFH progression. The translational potential of this article: This article gives novel methods for ONFH diagnosis and progression monitoring in a convenient and non-invasive way.http://www.sciencedirect.com/science/article/pii/S2214031X20300085Diagnosishiposteonecrosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yongheng Ye Yue Peng Peiheng He Qinqin Zhang Dongliang Xu |
spellingShingle |
Yongheng Ye Yue Peng Peiheng He Qinqin Zhang Dongliang Xu Urinary miRNAs as biomarkers for idiopathic osteonecrosis of femoral head: A multicentre study Journal of Orthopaedic Translation Diagnosis hip osteonecrosis |
author_facet |
Yongheng Ye Yue Peng Peiheng He Qinqin Zhang Dongliang Xu |
author_sort |
Yongheng Ye |
title |
Urinary miRNAs as biomarkers for idiopathic osteonecrosis of femoral head: A multicentre study |
title_short |
Urinary miRNAs as biomarkers for idiopathic osteonecrosis of femoral head: A multicentre study |
title_full |
Urinary miRNAs as biomarkers for idiopathic osteonecrosis of femoral head: A multicentre study |
title_fullStr |
Urinary miRNAs as biomarkers for idiopathic osteonecrosis of femoral head: A multicentre study |
title_full_unstemmed |
Urinary miRNAs as biomarkers for idiopathic osteonecrosis of femoral head: A multicentre study |
title_sort |
urinary mirnas as biomarkers for idiopathic osteonecrosis of femoral head: a multicentre study |
publisher |
Elsevier |
series |
Journal of Orthopaedic Translation |
issn |
2214-031X |
publishDate |
2021-01-01 |
description |
Objectives: Urinary microRNAs (miRNAs) have shown great diagnostic and prognostic values for multiple diseases. The profile of urinary miRNAs in patients with idiopathic osteonecrosis of femoral head (ONFH) is currently unclear. Methods: We first randomly chose ten patients with each Association Research Circulation Osseous (ARCO) stage (I, II, III and IV) and ten healthy participants from the entire cohorts for initial screening. The miRNA polymerase chain reaction (PCR) array was then performed to identify the differentially abundant miRNAs in urine of these participants. We then verified the findings in the entire cohort. Clinical features including age, gender, bone mass index (BMI), lesion size and stages were recorded. We then analysed the association between the level of urinary miRNAs and clinical features. Results: Our data indicated that there were 13 differentially abundant miRNAs among all groups. Urinary miR-150 demonstrated the highest diagnostic value among all candidates. Urinary miR-185 and miR-133a increased by ARCO staging. The levels of urinary miR-4824 abruptly decreased after femoral head collapse (ARCO stage III and IV). Urinary miR-144 was the only marker that correlated with lesion size. Conclusions: The levels of urinary miRNAs are valuable biomarkers for idiopathic ONFH. Given the noninvasive nature of this test, it is potentially useful for diagnosis and monitoring of idiopathic ONFH progression. The translational potential of this article: This article gives novel methods for ONFH diagnosis and progression monitoring in a convenient and non-invasive way. |
topic |
Diagnosis hip osteonecrosis |
url |
http://www.sciencedirect.com/science/article/pii/S2214031X20300085 |
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