Eosinophils increase neuron branching in human and murine skin and in vitro.
Cutaneous nerves are increased in atopic dermatitis, and itch is a prominent symptom. We studied the functional interactions between eosinophils and nerves in human and mouse skin and in culture. We demonstrated that human atopic dermatitis skin has eosinophil granule proteins present in the same re...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2011-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC3140999?pdf=render |
id |
doaj-abbda873cf5b41ae87e4fefc70a864d1 |
---|---|
record_format |
Article |
spelling |
doaj-abbda873cf5b41ae87e4fefc70a864d12020-11-25T01:52:50ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0167e2202910.1371/journal.pone.0022029Eosinophils increase neuron branching in human and murine skin and in vitro.Erin L FosterEric L SimpsonLorna J FredriksonJames J LeeNancy A LeeAllison D FryerDavid B JacobyCutaneous nerves are increased in atopic dermatitis, and itch is a prominent symptom. We studied the functional interactions between eosinophils and nerves in human and mouse skin and in culture. We demonstrated that human atopic dermatitis skin has eosinophil granule proteins present in the same region as increased nerves. Transgenic mice in which interleukin-5 (IL-5) expression is driven by a keratin-14 (K14) promoter had many eosinophils in the epidermis, and the number of nerves was also significantly increased in the epidermis. In co-cultures, eosinophils dramatically increased branching of sensory neurons isolated from the dorsal root ganglia (DRG) of mice. This effect did not occur in DRG neurons co-cultured with mast cells or with dead eosinophils. Physical contact of the eosinophils with the neurons was not required, and the effect was not blocked by an antibody to nerve growth factor. DRG neurons express eotaxin-1, ICAM-1 and VCAM-1, which may be important in the recruitment, binding, and activation of eosinophils in the region of cutaneous nerves. These data indicate a pathophysiological role for eosinophils in cutaneous nerve growth in atopic dermatitis, and suggest they may present a therapeutic target in atopic dermatitis and other eosinophilic skin conditions with neuronal symptoms such as itch.http://europepmc.org/articles/PMC3140999?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Erin L Foster Eric L Simpson Lorna J Fredrikson James J Lee Nancy A Lee Allison D Fryer David B Jacoby |
spellingShingle |
Erin L Foster Eric L Simpson Lorna J Fredrikson James J Lee Nancy A Lee Allison D Fryer David B Jacoby Eosinophils increase neuron branching in human and murine skin and in vitro. PLoS ONE |
author_facet |
Erin L Foster Eric L Simpson Lorna J Fredrikson James J Lee Nancy A Lee Allison D Fryer David B Jacoby |
author_sort |
Erin L Foster |
title |
Eosinophils increase neuron branching in human and murine skin and in vitro. |
title_short |
Eosinophils increase neuron branching in human and murine skin and in vitro. |
title_full |
Eosinophils increase neuron branching in human and murine skin and in vitro. |
title_fullStr |
Eosinophils increase neuron branching in human and murine skin and in vitro. |
title_full_unstemmed |
Eosinophils increase neuron branching in human and murine skin and in vitro. |
title_sort |
eosinophils increase neuron branching in human and murine skin and in vitro. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2011-01-01 |
description |
Cutaneous nerves are increased in atopic dermatitis, and itch is a prominent symptom. We studied the functional interactions between eosinophils and nerves in human and mouse skin and in culture. We demonstrated that human atopic dermatitis skin has eosinophil granule proteins present in the same region as increased nerves. Transgenic mice in which interleukin-5 (IL-5) expression is driven by a keratin-14 (K14) promoter had many eosinophils in the epidermis, and the number of nerves was also significantly increased in the epidermis. In co-cultures, eosinophils dramatically increased branching of sensory neurons isolated from the dorsal root ganglia (DRG) of mice. This effect did not occur in DRG neurons co-cultured with mast cells or with dead eosinophils. Physical contact of the eosinophils with the neurons was not required, and the effect was not blocked by an antibody to nerve growth factor. DRG neurons express eotaxin-1, ICAM-1 and VCAM-1, which may be important in the recruitment, binding, and activation of eosinophils in the region of cutaneous nerves. These data indicate a pathophysiological role for eosinophils in cutaneous nerve growth in atopic dermatitis, and suggest they may present a therapeutic target in atopic dermatitis and other eosinophilic skin conditions with neuronal symptoms such as itch. |
url |
http://europepmc.org/articles/PMC3140999?pdf=render |
work_keys_str_mv |
AT erinlfoster eosinophilsincreaseneuronbranchinginhumanandmurineskinandinvitro AT ericlsimpson eosinophilsincreaseneuronbranchinginhumanandmurineskinandinvitro AT lornajfredrikson eosinophilsincreaseneuronbranchinginhumanandmurineskinandinvitro AT jamesjlee eosinophilsincreaseneuronbranchinginhumanandmurineskinandinvitro AT nancyalee eosinophilsincreaseneuronbranchinginhumanandmurineskinandinvitro AT allisondfryer eosinophilsincreaseneuronbranchinginhumanandmurineskinandinvitro AT davidbjacoby eosinophilsincreaseneuronbranchinginhumanandmurineskinandinvitro |
_version_ |
1724992547334389760 |