The Construction and Immunoadjuvant Activities of the Oral Interleukin-17B Expressed by <i>Lactobacillus plantarum</i> NC8 Strain in the Infectious Bronchitis Virus Vaccination of Chickens
Interleukin-17B (IL-17B) is a protective cytokine of the IL-17 family and plays an essential role in the regulation of mucosal inflammation. However, little is known about the role of IL-17B in the control of viral infections. In this study, a recombinant <i>Lactobacillus plantarum</i>,...
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doaj-abc7fcfb793a4d63b5b5f25a98b1077d2020-11-25T02:26:28ZengMDPI AGVaccines2076-393X2020-06-01828228210.3390/vaccines8020282The Construction and Immunoadjuvant Activities of the Oral Interleukin-17B Expressed by <i>Lactobacillus plantarum</i> NC8 Strain in the Infectious Bronchitis Virus Vaccination of ChickensShaohua Guo0Junjie Peng1Yongle Xiao2Yanyan Liu3Weiwei Hao4Xin Yang5Hongning Wang6Rong Gao7College of Life Sciences, Sichuan University, Chengdu 610065, ChinaCollege of Life Sciences, Sichuan University, Chengdu 610065, ChinaCollege of Life Sciences, Sichuan University, Chengdu 610065, ChinaCollege of Life Sciences, Sichuan University, Chengdu 610065, ChinaSichuan Huapai Biopharmaceutical Company, Chengdu 610026, ChinaCollege of Life Sciences, Sichuan University, Chengdu 610065, ChinaCollege of Life Sciences, Sichuan University, Chengdu 610065, ChinaCollege of Life Sciences, Sichuan University, Chengdu 610065, ChinaInterleukin-17B (IL-17B) is a protective cytokine of the IL-17 family and plays an essential role in the regulation of mucosal inflammation. However, little is known about the role of IL-17B in the control of viral infections. In this study, a recombinant <i>Lactobacillus plantarum</i>, designated as NC8-ChIL17B, was constructed to express the chicken <i>IL-17B</i> (<i>ChIL-17B</i>) gene. The recombinant ChIL17B (rChIL17B) protein was about 14 kDa and was anchored to the surface of NC8 cells. In vitro, it was found that the rChIL17B protein inhibited the proliferation of the infectious bronchitis virus (IBV) through activation of nuclear factor kappa B (NF-κB) and the JAK (Janus kinase)-STAT (signal transducers and activators of transcription) signaling. Moreover, to evaluate the immunoadjuvant activities of NC8-ChIL17B, 40 three-day-old specific pathogen-free (SPF) chickens were divided into four groups. Three groups were orally vaccinated with fresh NC8, NC8-ChIL17B, and phosphate buffered saline (PBS), along with the infectious bronchitis virus vaccine, and the other group was the PBS-negative control. The results of the IBV-specific antibody titer and the concentration of the cytokines IL-2, IL-4, IL-6, and interferon gamma (IFN-γ) in sera, as well as the concentration of secretory immunoglobulin A (sIgA) in the tracheal and small intestinal mucosa, the number of cluster of differentiation 4 positive (CD4<sup>+</sup>) and cluster of differentiation 8 positive (CD8<sup>+</sup>) T cells in the blood, and the expression of immune-related genes all indicated that NC8-ChIL17B efficiently enhanced the humoral and cellular immune responses to IBV vaccine. Moreover, the viral loads in the NC8-ChIL17B- and IBV-vaccinated group were significantly lower than in the control groups, suggesting a significant promotion of the immunoprotection of IBV vaccination against the virulent IBV strain. Therefore, ChIL-17B is a promising, effective adjuvant candidate for chicken virus vaccines.https://www.mdpi.com/2076-393X/8/2/282chicken IL-17B<i>Lactobacillus plantarum</i>oral adjuvantIBV vaccineimmunity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shaohua Guo Junjie Peng Yongle Xiao Yanyan Liu Weiwei Hao Xin Yang Hongning Wang Rong Gao |
spellingShingle |
Shaohua Guo Junjie Peng Yongle Xiao Yanyan Liu Weiwei Hao Xin Yang Hongning Wang Rong Gao The Construction and Immunoadjuvant Activities of the Oral Interleukin-17B Expressed by <i>Lactobacillus plantarum</i> NC8 Strain in the Infectious Bronchitis Virus Vaccination of Chickens Vaccines chicken IL-17B <i>Lactobacillus plantarum</i> oral adjuvant IBV vaccine immunity |
author_facet |
Shaohua Guo Junjie Peng Yongle Xiao Yanyan Liu Weiwei Hao Xin Yang Hongning Wang Rong Gao |
author_sort |
Shaohua Guo |
title |
The Construction and Immunoadjuvant Activities of the Oral Interleukin-17B Expressed by <i>Lactobacillus plantarum</i> NC8 Strain in the Infectious Bronchitis Virus Vaccination of Chickens |
title_short |
The Construction and Immunoadjuvant Activities of the Oral Interleukin-17B Expressed by <i>Lactobacillus plantarum</i> NC8 Strain in the Infectious Bronchitis Virus Vaccination of Chickens |
title_full |
The Construction and Immunoadjuvant Activities of the Oral Interleukin-17B Expressed by <i>Lactobacillus plantarum</i> NC8 Strain in the Infectious Bronchitis Virus Vaccination of Chickens |
title_fullStr |
The Construction and Immunoadjuvant Activities of the Oral Interleukin-17B Expressed by <i>Lactobacillus plantarum</i> NC8 Strain in the Infectious Bronchitis Virus Vaccination of Chickens |
title_full_unstemmed |
The Construction and Immunoadjuvant Activities of the Oral Interleukin-17B Expressed by <i>Lactobacillus plantarum</i> NC8 Strain in the Infectious Bronchitis Virus Vaccination of Chickens |
title_sort |
construction and immunoadjuvant activities of the oral interleukin-17b expressed by <i>lactobacillus plantarum</i> nc8 strain in the infectious bronchitis virus vaccination of chickens |
publisher |
MDPI AG |
series |
Vaccines |
issn |
2076-393X |
publishDate |
2020-06-01 |
description |
Interleukin-17B (IL-17B) is a protective cytokine of the IL-17 family and plays an essential role in the regulation of mucosal inflammation. However, little is known about the role of IL-17B in the control of viral infections. In this study, a recombinant <i>Lactobacillus plantarum</i>, designated as NC8-ChIL17B, was constructed to express the chicken <i>IL-17B</i> (<i>ChIL-17B</i>) gene. The recombinant ChIL17B (rChIL17B) protein was about 14 kDa and was anchored to the surface of NC8 cells. In vitro, it was found that the rChIL17B protein inhibited the proliferation of the infectious bronchitis virus (IBV) through activation of nuclear factor kappa B (NF-κB) and the JAK (Janus kinase)-STAT (signal transducers and activators of transcription) signaling. Moreover, to evaluate the immunoadjuvant activities of NC8-ChIL17B, 40 three-day-old specific pathogen-free (SPF) chickens were divided into four groups. Three groups were orally vaccinated with fresh NC8, NC8-ChIL17B, and phosphate buffered saline (PBS), along with the infectious bronchitis virus vaccine, and the other group was the PBS-negative control. The results of the IBV-specific antibody titer and the concentration of the cytokines IL-2, IL-4, IL-6, and interferon gamma (IFN-γ) in sera, as well as the concentration of secretory immunoglobulin A (sIgA) in the tracheal and small intestinal mucosa, the number of cluster of differentiation 4 positive (CD4<sup>+</sup>) and cluster of differentiation 8 positive (CD8<sup>+</sup>) T cells in the blood, and the expression of immune-related genes all indicated that NC8-ChIL17B efficiently enhanced the humoral and cellular immune responses to IBV vaccine. Moreover, the viral loads in the NC8-ChIL17B- and IBV-vaccinated group were significantly lower than in the control groups, suggesting a significant promotion of the immunoprotection of IBV vaccination against the virulent IBV strain. Therefore, ChIL-17B is a promising, effective adjuvant candidate for chicken virus vaccines. |
topic |
chicken IL-17B <i>Lactobacillus plantarum</i> oral adjuvant IBV vaccine immunity |
url |
https://www.mdpi.com/2076-393X/8/2/282 |
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