The Marmoset as an Animal Model of Influenza: Infection With A(H1N1)pdm09 and Highly Pathogenic A(H5N1) Viruses via the Conventional or Tracheal Spray Route
To control infectious diseases in humans, it is important to understand the pathogenicity of the infecting organism(s). Although non-human primates, such as cynomolgus and rhesus macaques, have been used for influenza virus infection models, their size can limit their use in confined animal faciliti...
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doaj-abe0f222cd88453689393b0b196ba2852020-11-24T23:45:13ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-05-01910.3389/fmicb.2018.00844350028The Marmoset as an Animal Model of Influenza: Infection With A(H1N1)pdm09 and Highly Pathogenic A(H5N1) Viruses via the Conventional or Tracheal Spray RouteKiyoko Iwatsuki-Horimoto0Noriko Nakajima1Maki Kiso2Kenta Takahashi3Mutsumi Ito4Takashi Inoue5Machiko Horiuchi6Norio Okahara7Erika Sasaki8Erika Sasaki9Hideki Hasegawa10Yoshihiro Kawaoka11Yoshihiro Kawaoka12Yoshihiro Kawaoka13Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, JapanDepartment of Pathology, National Institute of Infectious Diseases, Tokyo, JapanDivision of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, JapanDepartment of Pathology, National Institute of Infectious Diseases, Tokyo, JapanDivision of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, JapanMarmoset Research Department, Central Institute for Experimental Animals, Kawasaki, JapanBioSciences Group, Summit Pharmaceuticals International Corporation, Tokyo, JapanMarmoset Research Department, Central Institute for Experimental Animals, Kawasaki, JapanMarmoset Research Department, Central Institute for Experimental Animals, Kawasaki, JapanKeio Advanced Research Center, Keio University, Tokyo, JapanDepartment of Pathology, National Institute of Infectious Diseases, Tokyo, JapanDivision of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, JapanInfluenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, United StatesDepartment of Special Pathogens, International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, Tokyo, JapanTo control infectious diseases in humans, it is important to understand the pathogenicity of the infecting organism(s). Although non-human primates, such as cynomolgus and rhesus macaques, have been used for influenza virus infection models, their size can limit their use in confined animal facilities. In this study, we investigated the susceptibility of marmosets to influenza viruses to assess the possibility of using these animals as a non-human primate model for influenza research. We first used an influenza A (H1N1)pdm09 virus to compare two inoculation routes: the conventional route, via a combination of the intratracheal, intranasal, ocular, and oral routes; and the tracheal spray route. In marmosets inoculated via the tracheal spray route, we found inflammation throughout the lungs and trachea. In contrast, in marmosets inoculated via the conventional route, the inflammation was confined to roughly the center of the lung. These data suggest that the tracheal spray route may be more suitable than the conventional route to inoculate marmosets with influenza viruses. We also tested an influenza A(H5N1) highly pathogenic avian influenza (HPAI) virus and found that some marmosets inoculated with this virus via the tracheal spray route showed weight loss, decreased body temperature, and loss of appetite and activity. The replication of this H5N1 virus in respiratory organs was confirmed. These results indicate the potential of marmosets as an animal model for infection with seasonal or HPAI viruses.http://journal.frontiersin.org/article/10.3389/fmicb.2018.00844/fullinfluenza virusanimal modelmarmosetnon-human primateA(H5N1) |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kiyoko Iwatsuki-Horimoto Noriko Nakajima Maki Kiso Kenta Takahashi Mutsumi Ito Takashi Inoue Machiko Horiuchi Norio Okahara Erika Sasaki Erika Sasaki Hideki Hasegawa Yoshihiro Kawaoka Yoshihiro Kawaoka Yoshihiro Kawaoka |
spellingShingle |
Kiyoko Iwatsuki-Horimoto Noriko Nakajima Maki Kiso Kenta Takahashi Mutsumi Ito Takashi Inoue Machiko Horiuchi Norio Okahara Erika Sasaki Erika Sasaki Hideki Hasegawa Yoshihiro Kawaoka Yoshihiro Kawaoka Yoshihiro Kawaoka The Marmoset as an Animal Model of Influenza: Infection With A(H1N1)pdm09 and Highly Pathogenic A(H5N1) Viruses via the Conventional or Tracheal Spray Route Frontiers in Microbiology influenza virus animal model marmoset non-human primate A(H5N1) |
author_facet |
Kiyoko Iwatsuki-Horimoto Noriko Nakajima Maki Kiso Kenta Takahashi Mutsumi Ito Takashi Inoue Machiko Horiuchi Norio Okahara Erika Sasaki Erika Sasaki Hideki Hasegawa Yoshihiro Kawaoka Yoshihiro Kawaoka Yoshihiro Kawaoka |
author_sort |
Kiyoko Iwatsuki-Horimoto |
title |
The Marmoset as an Animal Model of Influenza: Infection With A(H1N1)pdm09 and Highly Pathogenic A(H5N1) Viruses via the Conventional or Tracheal Spray Route |
title_short |
The Marmoset as an Animal Model of Influenza: Infection With A(H1N1)pdm09 and Highly Pathogenic A(H5N1) Viruses via the Conventional or Tracheal Spray Route |
title_full |
The Marmoset as an Animal Model of Influenza: Infection With A(H1N1)pdm09 and Highly Pathogenic A(H5N1) Viruses via the Conventional or Tracheal Spray Route |
title_fullStr |
The Marmoset as an Animal Model of Influenza: Infection With A(H1N1)pdm09 and Highly Pathogenic A(H5N1) Viruses via the Conventional or Tracheal Spray Route |
title_full_unstemmed |
The Marmoset as an Animal Model of Influenza: Infection With A(H1N1)pdm09 and Highly Pathogenic A(H5N1) Viruses via the Conventional or Tracheal Spray Route |
title_sort |
marmoset as an animal model of influenza: infection with a(h1n1)pdm09 and highly pathogenic a(h5n1) viruses via the conventional or tracheal spray route |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2018-05-01 |
description |
To control infectious diseases in humans, it is important to understand the pathogenicity of the infecting organism(s). Although non-human primates, such as cynomolgus and rhesus macaques, have been used for influenza virus infection models, their size can limit their use in confined animal facilities. In this study, we investigated the susceptibility of marmosets to influenza viruses to assess the possibility of using these animals as a non-human primate model for influenza research. We first used an influenza A (H1N1)pdm09 virus to compare two inoculation routes: the conventional route, via a combination of the intratracheal, intranasal, ocular, and oral routes; and the tracheal spray route. In marmosets inoculated via the tracheal spray route, we found inflammation throughout the lungs and trachea. In contrast, in marmosets inoculated via the conventional route, the inflammation was confined to roughly the center of the lung. These data suggest that the tracheal spray route may be more suitable than the conventional route to inoculate marmosets with influenza viruses. We also tested an influenza A(H5N1) highly pathogenic avian influenza (HPAI) virus and found that some marmosets inoculated with this virus via the tracheal spray route showed weight loss, decreased body temperature, and loss of appetite and activity. The replication of this H5N1 virus in respiratory organs was confirmed. These results indicate the potential of marmosets as an animal model for infection with seasonal or HPAI viruses. |
topic |
influenza virus animal model marmoset non-human primate A(H5N1) |
url |
http://journal.frontiersin.org/article/10.3389/fmicb.2018.00844/full |
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