Prediction of protein structure of novel protein (116 kDa) from human sperm membrane
Objective: To predict the protein similarity, biological function and structure of 116 kDa protein that was isolated from human sperm membrane of acrosome by using in silico. Methods: Predictions for 116 kDa protein similarity was done through comparative analysis of its isoelectric point (pI) and m...
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Wolters Kluwer Medknow Publications
2018-01-01
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doaj-abe828b8309a466f9a7f4e0e5328d3532020-11-24T23:07:24ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Reproduction2305-05002305-05192018-01-017313613810.4103/2305-0500.233575Prediction of protein structure of novel protein (116 kDa) from human sperm membraneUmie LestariWidodoSutiman Bambang SumitroObjective: To predict the protein similarity, biological function and structure of 116 kDa protein that was isolated from human sperm membrane of acrosome by using in silico. Methods: Predictions for 116 kDa protein similarity was done through comparative analysis of its isoelectric point (pI) and molecular weight to the UniProtKB/TrEMBL protein database. The three-dimensional structure of the protein was built using SWISS-MODEL. Results: The 116 kDa protein with pI 4.4 was expected to have similarities with CDH23 proteins that had pI 4.38, thus they likely had similar physical and biochemical properties. The predicted protein had an extracellular N-terminal that likely acted as a receptor, and showed cell recognition characteristic, playing a role in fertilization. Conclusions: The 116 kDa protein isolated from acrosome membrane has similarity on its biophysical character to the CDH23.http://www.apjr.net/article.asp?issn=2305-0500;year=2018;volume=7;issue=3;spage=136;epage=138;aulast=Lestari116 kDa proteinAcrosomeBiochemical propertiesCDH23 proteinHuman sperm membrane |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Umie Lestari Widodo Sutiman Bambang Sumitro |
spellingShingle |
Umie Lestari Widodo Sutiman Bambang Sumitro Prediction of protein structure of novel protein (116 kDa) from human sperm membrane Asian Pacific Journal of Reproduction 116 kDa protein Acrosome Biochemical properties CDH23 protein Human sperm membrane |
author_facet |
Umie Lestari Widodo Sutiman Bambang Sumitro |
author_sort |
Umie Lestari |
title |
Prediction of protein structure of novel protein (116 kDa) from human sperm membrane |
title_short |
Prediction of protein structure of novel protein (116 kDa) from human sperm membrane |
title_full |
Prediction of protein structure of novel protein (116 kDa) from human sperm membrane |
title_fullStr |
Prediction of protein structure of novel protein (116 kDa) from human sperm membrane |
title_full_unstemmed |
Prediction of protein structure of novel protein (116 kDa) from human sperm membrane |
title_sort |
prediction of protein structure of novel protein (116 kda) from human sperm membrane |
publisher |
Wolters Kluwer Medknow Publications |
series |
Asian Pacific Journal of Reproduction |
issn |
2305-0500 2305-0519 |
publishDate |
2018-01-01 |
description |
Objective: To predict the protein similarity, biological function and structure of 116 kDa protein that was isolated from human sperm membrane of acrosome by using in silico. Methods: Predictions for 116 kDa protein similarity was done through comparative analysis of its isoelectric point (pI) and molecular weight to the UniProtKB/TrEMBL protein database. The three-dimensional structure of the protein was built using SWISS-MODEL. Results: The 116 kDa protein with pI 4.4 was expected to have similarities with CDH23 proteins that had pI 4.38, thus they likely had similar physical and biochemical properties. The predicted protein had an extracellular N-terminal that likely acted as a receptor, and showed cell recognition characteristic, playing a role in fertilization. Conclusions: The 116 kDa protein isolated from acrosome membrane has similarity on its biophysical character to the CDH23. |
topic |
116 kDa protein Acrosome Biochemical properties CDH23 protein Human sperm membrane |
url |
http://www.apjr.net/article.asp?issn=2305-0500;year=2018;volume=7;issue=3;spage=136;epage=138;aulast=Lestari |
work_keys_str_mv |
AT umielestari predictionofproteinstructureofnovelprotein116kdafromhumanspermmembrane AT widodo predictionofproteinstructureofnovelprotein116kdafromhumanspermmembrane AT sutimanbambangsumitro predictionofproteinstructureofnovelprotein116kdafromhumanspermmembrane |
_version_ |
1725618577377067008 |