Engineering Lipid Bilayer Membranes for Protein Studies
Lipid membranes regulate the flow of nutrients and communication signaling between cells and protect the sub-cellular structures. Recent attempts to fabricate artificial systems using nanostructures that mimic the physiological properties of natural lipid bilayer membranes (LBM) fused with transmemb...
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Online Access: | http://www.mdpi.com/1422-0067/14/11/21561 |
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doaj-abea9241e8f64f3b975cf79a13e1266e2020-11-24T21:52:06ZengMDPI AGInternational Journal of Molecular Sciences1422-00672013-10-011411215612159710.3390/ijms141121561Engineering Lipid Bilayer Membranes for Protein StudiesMuhammad Shuja KhanNoura Sayed DosokyJohn Dalton WilliamsLipid membranes regulate the flow of nutrients and communication signaling between cells and protect the sub-cellular structures. Recent attempts to fabricate artificial systems using nanostructures that mimic the physiological properties of natural lipid bilayer membranes (LBM) fused with transmembrane proteins have helped demonstrate the importance of temperature, pH, ionic strength, adsorption behavior, conformational reorientation and surface density in cellular membranes which all affect the incorporation of proteins on solid surfaces. Much of this work is performed on artificial templates made of polymer sponges or porous materials based on alumina, mica, and porous silicon (PSi) surfaces. For example, porous silicon materials have high biocompatibility, biodegradability, and photoluminescence, which allow them to be used both as a support structure for lipid bilayers or a template to measure the electrochemical functionality of living cells grown over the surface as in vivo. The variety of these media, coupled with the complex physiological conditions present in living systems, warrant a summary and prospectus detailing which artificial systems provide the most promise for different biological conditions. This study summarizes the use of electrochemical impedance spectroscopy (EIS) data on artificial biological membranes that are closely matched with previously published biological systems using both black lipid membrane and patch clamp techniques.http://www.mdpi.com/1422-0067/14/11/21561lipid bilayer membranenanoporous materialssilicontransmembrane proteinselectrochemical impedance spectroscopy |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Muhammad Shuja Khan Noura Sayed Dosoky John Dalton Williams |
spellingShingle |
Muhammad Shuja Khan Noura Sayed Dosoky John Dalton Williams Engineering Lipid Bilayer Membranes for Protein Studies International Journal of Molecular Sciences lipid bilayer membrane nanoporous materials silicon transmembrane proteins electrochemical impedance spectroscopy |
author_facet |
Muhammad Shuja Khan Noura Sayed Dosoky John Dalton Williams |
author_sort |
Muhammad Shuja Khan |
title |
Engineering Lipid Bilayer Membranes for Protein Studies |
title_short |
Engineering Lipid Bilayer Membranes for Protein Studies |
title_full |
Engineering Lipid Bilayer Membranes for Protein Studies |
title_fullStr |
Engineering Lipid Bilayer Membranes for Protein Studies |
title_full_unstemmed |
Engineering Lipid Bilayer Membranes for Protein Studies |
title_sort |
engineering lipid bilayer membranes for protein studies |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2013-10-01 |
description |
Lipid membranes regulate the flow of nutrients and communication signaling between cells and protect the sub-cellular structures. Recent attempts to fabricate artificial systems using nanostructures that mimic the physiological properties of natural lipid bilayer membranes (LBM) fused with transmembrane proteins have helped demonstrate the importance of temperature, pH, ionic strength, adsorption behavior, conformational reorientation and surface density in cellular membranes which all affect the incorporation of proteins on solid surfaces. Much of this work is performed on artificial templates made of polymer sponges or porous materials based on alumina, mica, and porous silicon (PSi) surfaces. For example, porous silicon materials have high biocompatibility, biodegradability, and photoluminescence, which allow them to be used both as a support structure for lipid bilayers or a template to measure the electrochemical functionality of living cells grown over the surface as in vivo. The variety of these media, coupled with the complex physiological conditions present in living systems, warrant a summary and prospectus detailing which artificial systems provide the most promise for different biological conditions. This study summarizes the use of electrochemical impedance spectroscopy (EIS) data on artificial biological membranes that are closely matched with previously published biological systems using both black lipid membrane and patch clamp techniques. |
topic |
lipid bilayer membrane nanoporous materials silicon transmembrane proteins electrochemical impedance spectroscopy |
url |
http://www.mdpi.com/1422-0067/14/11/21561 |
work_keys_str_mv |
AT muhammadshujakhan engineeringlipidbilayermembranesforproteinstudies AT nourasayeddosoky engineeringlipidbilayermembranesforproteinstudies AT johndaltonwilliams engineeringlipidbilayermembranesforproteinstudies |
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