In situ protein detection for companion diagnostics
The emergence of targeted therapies for cancer has created a need for the development of companion diagnostic tests. Assays developed in recent years are aimed at determining both the effectiveness and safety of specific drugs for a defined group of patients, thus, enabling the more efficient design...
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Frontiers Media S.A.
2013-10-01
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Online Access: | http://journal.frontiersin.org/Journal/10.3389/fonc.2013.00271/full |
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doaj-ac16f33c066c4da8a9658ac5f0688afe2020-11-24T23:58:53ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2013-10-01310.3389/fonc.2013.0027170411In situ protein detection for companion diagnosticsGabriela eGremel0Karin eGrannas1Lesley Ann Sutton2Fredrik ePontén3Agata eZieba4Uppsala UniversityUppsala UniversityUppsala UniversityUppsala UniversityUppsala UniversityThe emergence of targeted therapies for cancer has created a need for the development of companion diagnostic tests. Assays developed in recent years are aimed at determining both the effectiveness and safety of specific drugs for a defined group of patients, thus, enabling the more efficient design of clinical trials and also supporting physicians when making treatment-related decisions. Immunohistochemistry is a widely accepted method for protein expression analyses in human tissues. Immunohistochemical assays, used to localize and quantitate relative protein expression levels within a morphological context, are frequently used as companion diagnostics during clinical trials and also following drug approval. Herein, we describe established immunochemistry-based methods and their application in routine diagnostics. We also explore the possibility of using immunohistochemistry to detect specific protein mutations in addition to DNA-based tests. Finally, we review alternative protein binders and proximity ligation assays and discuss their potential to facilitate the development of novel, targeted therapies against cancer.http://journal.frontiersin.org/Journal/10.3389/fonc.2013.00271/fullImmunohistochemistryHER2companion diagnosticsalternative bindersproximity ligation assays |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gabriela eGremel Karin eGrannas Lesley Ann Sutton Fredrik ePontén Agata eZieba |
spellingShingle |
Gabriela eGremel Karin eGrannas Lesley Ann Sutton Fredrik ePontén Agata eZieba In situ protein detection for companion diagnostics Frontiers in Oncology Immunohistochemistry HER2 companion diagnostics alternative binders proximity ligation assays |
author_facet |
Gabriela eGremel Karin eGrannas Lesley Ann Sutton Fredrik ePontén Agata eZieba |
author_sort |
Gabriela eGremel |
title |
In situ protein detection for companion diagnostics |
title_short |
In situ protein detection for companion diagnostics |
title_full |
In situ protein detection for companion diagnostics |
title_fullStr |
In situ protein detection for companion diagnostics |
title_full_unstemmed |
In situ protein detection for companion diagnostics |
title_sort |
in situ protein detection for companion diagnostics |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Oncology |
issn |
2234-943X |
publishDate |
2013-10-01 |
description |
The emergence of targeted therapies for cancer has created a need for the development of companion diagnostic tests. Assays developed in recent years are aimed at determining both the effectiveness and safety of specific drugs for a defined group of patients, thus, enabling the more efficient design of clinical trials and also supporting physicians when making treatment-related decisions. Immunohistochemistry is a widely accepted method for protein expression analyses in human tissues. Immunohistochemical assays, used to localize and quantitate relative protein expression levels within a morphological context, are frequently used as companion diagnostics during clinical trials and also following drug approval. Herein, we describe established immunochemistry-based methods and their application in routine diagnostics. We also explore the possibility of using immunohistochemistry to detect specific protein mutations in addition to DNA-based tests. Finally, we review alternative protein binders and proximity ligation assays and discuss their potential to facilitate the development of novel, targeted therapies against cancer. |
topic |
Immunohistochemistry HER2 companion diagnostics alternative binders proximity ligation assays |
url |
http://journal.frontiersin.org/Journal/10.3389/fonc.2013.00271/full |
work_keys_str_mv |
AT gabrielaegremel insituproteindetectionforcompaniondiagnostics AT karinegrannas insituproteindetectionforcompaniondiagnostics AT lesleyannsutton insituproteindetectionforcompaniondiagnostics AT fredrikeponten insituproteindetectionforcompaniondiagnostics AT agataezieba insituproteindetectionforcompaniondiagnostics |
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1725449194000351232 |