Rapid Assays for Detection of Clostridium difficile and Its Toxins in Hospitalized Patients
In this study, the rapid detection of Clostridium difficile and its toxins (A/B) in admitting patients in hospitals were offered the possibility of prevention of the bacterium spread into other patients. This bacterium is the most common cause of hospital-acquired diarrhea in patients treated wit...
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Journal of Pure and Applied Microbiology
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doaj-ac24764b25ab4512b0038b339c87e0ca2021-10-02T17:24:19ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2018-09-011231247125410.22207/JPAM.12.3.26Rapid Assays for Detection of Clostridium difficile and Its Toxins in Hospitalized PatientsMahmoud AbdEl-Mongy0Said El-Feky1Heba Masoud2Amira El-Hendi3Microbial Biotechnology Department, Genetic Engineering and Biotechnology Institute, University of Sadat City. Sadat City, Egypt.Biochemistry Department, Damanhur Medical National Institute, Egypt.Microbial Biotechnology Department, Genetic Engineering and Biotechnology Institute, University of Sadat City. Sadat City, Egypt.Clinical pathology Department, Faculty of Medicine, Minoufiya University, Shibin El-Kom, Egypt.In this study, the rapid detection of Clostridium difficile and its toxins (A/B) in admitting patients in hospitals were offered the possibility of prevention of the bacterium spread into other patients. This bacterium is the most common cause of hospital-acquired diarrhea in patients treated with antibiotics, chemotherapeutic agents and other drugs that alter the normal equilibrium of the intestinal flora. The Stool samples were cultured on specific agar media with anaerobically growth equipment. The C. difficile toxins A and B were detected in fecal samples by using enzyme-linked immunosorbent assay (EIA). In this study, there was a higher incidence of C. Difficile infection among the hospitalized patients in different hospital wards, especially in the renal ward, hepatic ward and oncology ward. The using of EIA technique for pathogen detection is very useful and significant method for the rapid detection of the C. difficile strains.https://microbiologyjournal.org/rapid-assays-for-detection-of-clostridium-difficile-and-its-toxins-in-hospitalized-patients/clostridium difficiletoxins a/brenal patientshepatic patientsoncology patients |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mahmoud AbdEl-Mongy Said El-Feky Heba Masoud Amira El-Hendi |
spellingShingle |
Mahmoud AbdEl-Mongy Said El-Feky Heba Masoud Amira El-Hendi Rapid Assays for Detection of Clostridium difficile and Its Toxins in Hospitalized Patients Journal of Pure and Applied Microbiology clostridium difficile toxins a/b renal patients hepatic patients oncology patients |
author_facet |
Mahmoud AbdEl-Mongy Said El-Feky Heba Masoud Amira El-Hendi |
author_sort |
Mahmoud AbdEl-Mongy |
title |
Rapid Assays for Detection of Clostridium difficile and Its Toxins in Hospitalized Patients |
title_short |
Rapid Assays for Detection of Clostridium difficile and Its Toxins in Hospitalized Patients |
title_full |
Rapid Assays for Detection of Clostridium difficile and Its Toxins in Hospitalized Patients |
title_fullStr |
Rapid Assays for Detection of Clostridium difficile and Its Toxins in Hospitalized Patients |
title_full_unstemmed |
Rapid Assays for Detection of Clostridium difficile and Its Toxins in Hospitalized Patients |
title_sort |
rapid assays for detection of clostridium difficile and its toxins in hospitalized patients |
publisher |
Journal of Pure and Applied Microbiology |
series |
Journal of Pure and Applied Microbiology |
issn |
0973-7510 2581-690X |
publishDate |
2018-09-01 |
description |
In this study, the rapid detection of Clostridium difficile and its toxins (A/B) in
admitting patients in hospitals were offered the possibility of prevention of the bacterium spread
into other patients. This bacterium is the most common cause of hospital-acquired diarrhea in
patients treated with antibiotics, chemotherapeutic agents and other drugs that alter the normal
equilibrium of the intestinal flora. The Stool samples were cultured on specific agar media with
anaerobically growth equipment. The C. difficile toxins A and B were detected in fecal samples
by using enzyme-linked immunosorbent assay (EIA). In this study, there was a higher incidence
of C. Difficile infection among the hospitalized patients in different hospital wards, especially
in the renal ward, hepatic ward and oncology ward. The using of EIA technique for pathogen
detection is very useful and significant method for the rapid detection of the C. difficile strains. |
topic |
clostridium difficile toxins a/b renal patients hepatic patients oncology patients |
url |
https://microbiologyjournal.org/rapid-assays-for-detection-of-clostridium-difficile-and-its-toxins-in-hospitalized-patients/ |
work_keys_str_mv |
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