Association between Interferon Response and Protective Efficacy of NS1-Truncated Mutants as Influenza Vaccine Candidates in Chickens.
Influenza virus mutants that encode C-terminally truncated NS1 proteins (NS1-truncated mutants) are attractive candidates for avian live attenuated influenza vaccine (LAIV) development because they are both attenuated and immunogenic in chickens. We previously showed that a high protective efficacy...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2016-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC4892592?pdf=render |
id |
doaj-ac32d86d2612420b8d2714ef40fc63c3 |
---|---|
record_format |
Article |
spelling |
doaj-ac32d86d2612420b8d2714ef40fc63c32020-11-25T02:54:57ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01116e015660310.1371/journal.pone.0156603Association between Interferon Response and Protective Efficacy of NS1-Truncated Mutants as Influenza Vaccine Candidates in Chickens.Hyesun JangJohn M NgunjiriChang-Won LeeInfluenza virus mutants that encode C-terminally truncated NS1 proteins (NS1-truncated mutants) are attractive candidates for avian live attenuated influenza vaccine (LAIV) development because they are both attenuated and immunogenic in chickens. We previously showed that a high protective efficacy of NS1-truncated LAIV in chickens corresponds with induction of high levels of type I interferon (IFN) responses in chicken embryonic fibroblast cells. In this study, we investigated the relationship between induction of IFN and IFN-stimulated gene responses in vivo and the immunogenicity and protective efficacy of NS1-truncated LAIV. Our data demonstrates that accelerated antibody induction and protective efficacy of NS1-truncated LAIV correlates well with upregulation of IFN-stimulated genes. Further, through oral administration of recombinant chicken IFN alpha in drinking water, we provide direct evidence that type I IFN can promote rapid induction of adaptive immune responses and protective efficacy of influenza vaccine in chickens.http://europepmc.org/articles/PMC4892592?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hyesun Jang John M Ngunjiri Chang-Won Lee |
spellingShingle |
Hyesun Jang John M Ngunjiri Chang-Won Lee Association between Interferon Response and Protective Efficacy of NS1-Truncated Mutants as Influenza Vaccine Candidates in Chickens. PLoS ONE |
author_facet |
Hyesun Jang John M Ngunjiri Chang-Won Lee |
author_sort |
Hyesun Jang |
title |
Association between Interferon Response and Protective Efficacy of NS1-Truncated Mutants as Influenza Vaccine Candidates in Chickens. |
title_short |
Association between Interferon Response and Protective Efficacy of NS1-Truncated Mutants as Influenza Vaccine Candidates in Chickens. |
title_full |
Association between Interferon Response and Protective Efficacy of NS1-Truncated Mutants as Influenza Vaccine Candidates in Chickens. |
title_fullStr |
Association between Interferon Response and Protective Efficacy of NS1-Truncated Mutants as Influenza Vaccine Candidates in Chickens. |
title_full_unstemmed |
Association between Interferon Response and Protective Efficacy of NS1-Truncated Mutants as Influenza Vaccine Candidates in Chickens. |
title_sort |
association between interferon response and protective efficacy of ns1-truncated mutants as influenza vaccine candidates in chickens. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2016-01-01 |
description |
Influenza virus mutants that encode C-terminally truncated NS1 proteins (NS1-truncated mutants) are attractive candidates for avian live attenuated influenza vaccine (LAIV) development because they are both attenuated and immunogenic in chickens. We previously showed that a high protective efficacy of NS1-truncated LAIV in chickens corresponds with induction of high levels of type I interferon (IFN) responses in chicken embryonic fibroblast cells. In this study, we investigated the relationship between induction of IFN and IFN-stimulated gene responses in vivo and the immunogenicity and protective efficacy of NS1-truncated LAIV. Our data demonstrates that accelerated antibody induction and protective efficacy of NS1-truncated LAIV correlates well with upregulation of IFN-stimulated genes. Further, through oral administration of recombinant chicken IFN alpha in drinking water, we provide direct evidence that type I IFN can promote rapid induction of adaptive immune responses and protective efficacy of influenza vaccine in chickens. |
url |
http://europepmc.org/articles/PMC4892592?pdf=render |
work_keys_str_mv |
AT hyesunjang associationbetweeninterferonresponseandprotectiveefficacyofns1truncatedmutantsasinfluenzavaccinecandidatesinchickens AT johnmngunjiri associationbetweeninterferonresponseandprotectiveefficacyofns1truncatedmutantsasinfluenzavaccinecandidatesinchickens AT changwonlee associationbetweeninterferonresponseandprotectiveefficacyofns1truncatedmutantsasinfluenzavaccinecandidatesinchickens |
_version_ |
1724718826736582656 |