Cloning and identification of PK15 cells for enhanced replication of classical swine fever virus

Classical swine fever virus (CSFV) causes an economically important and highly contagious disease of pigs, leading to economic losses around the world. Attenuated live vaccines with CSFV antigens have played an important role in the prevention and control of the disease. Porcine kidney 15 (PK15) cel...

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Main Authors: Yin Mei, Hu Dongfang, Li Peng, Kong Lingyun, Ning Hongmei, Yue Feng, Jiang Jinqing, Wang Xuannian
Format: Article
Language:English
Published: Sciendo 2020-03-01
Series:Journal of Veterinary Research
Subjects:
Online Access:https://doi.org/10.2478/jvetres-2020-0020
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spelling doaj-ac4316986c96497d89dddc3238b5caba2021-09-06T19:41:50ZengSciendoJournal of Veterinary Research2450-86082020-03-0164191410.2478/jvetres-2020-0020jvetres-2020-0020Cloning and identification of PK15 cells for enhanced replication of classical swine fever virusYin Mei0Hu Dongfang1Li Peng2Kong Lingyun3Ning Hongmei4Yue Feng5Jiang Jinqing6Wang Xuannian7College of Animal Science and Technology, Henan Institute of Science and Technology, Xinxiang, Henan, 453003, ChinaCollege of Animal Science and Technology, Henan Institute of Science and Technology, Xinxiang, Henan, 453003, ChinaCollege of Life Science and Technology, Xinxiang University, Xinxiang, Henan, 453003, ChinaXinxiangxian Agriculture and Animal Husbandry Bureau (Xinxiangxian Forestry Bureau), Xinxiang, Henan, 453700, ChinaCollege of Animal Science and Technology, Henan Institute of Science and Technology, Xinxiang, Henan, 453003, ChinaCollege of Life Science and Technology, Xinxiang University, Xinxiang, Henan, 453003, ChinaCollege of Animal Science and Technology, Henan Institute of Science and Technology, Xinxiang, Henan, 453003, ChinaCollege of Life Science and Technology, Xinxiang University, Xinxiang, Henan, 453003, ChinaClassical swine fever virus (CSFV) causes an economically important and highly contagious disease of pigs, leading to economic losses around the world. Attenuated live vaccines with CSFV antigens have played an important role in the prevention and control of the disease. Porcine kidney 15 (PK15) cells have been widely used for the propagation of CSFV, but this cell line is not efficient or homogeneously susceptible to viral infection.https://doi.org/10.2478/jvetres-2020-0020classical swine fever viruspk15high-permissive cellscell cloningvaccine production
collection DOAJ
language English
format Article
sources DOAJ
author Yin Mei
Hu Dongfang
Li Peng
Kong Lingyun
Ning Hongmei
Yue Feng
Jiang Jinqing
Wang Xuannian
spellingShingle Yin Mei
Hu Dongfang
Li Peng
Kong Lingyun
Ning Hongmei
Yue Feng
Jiang Jinqing
Wang Xuannian
Cloning and identification of PK15 cells for enhanced replication of classical swine fever virus
Journal of Veterinary Research
classical swine fever virus
pk15
high-permissive cells
cell cloning
vaccine production
author_facet Yin Mei
Hu Dongfang
Li Peng
Kong Lingyun
Ning Hongmei
Yue Feng
Jiang Jinqing
Wang Xuannian
author_sort Yin Mei
title Cloning and identification of PK15 cells for enhanced replication of classical swine fever virus
title_short Cloning and identification of PK15 cells for enhanced replication of classical swine fever virus
title_full Cloning and identification of PK15 cells for enhanced replication of classical swine fever virus
title_fullStr Cloning and identification of PK15 cells for enhanced replication of classical swine fever virus
title_full_unstemmed Cloning and identification of PK15 cells for enhanced replication of classical swine fever virus
title_sort cloning and identification of pk15 cells for enhanced replication of classical swine fever virus
publisher Sciendo
series Journal of Veterinary Research
issn 2450-8608
publishDate 2020-03-01
description Classical swine fever virus (CSFV) causes an economically important and highly contagious disease of pigs, leading to economic losses around the world. Attenuated live vaccines with CSFV antigens have played an important role in the prevention and control of the disease. Porcine kidney 15 (PK15) cells have been widely used for the propagation of CSFV, but this cell line is not efficient or homogeneously susceptible to viral infection.
topic classical swine fever virus
pk15
high-permissive cells
cell cloning
vaccine production
url https://doi.org/10.2478/jvetres-2020-0020
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AT hudongfang cloningandidentificationofpk15cellsforenhancedreplicationofclassicalswinefevervirus
AT lipeng cloningandidentificationofpk15cellsforenhancedreplicationofclassicalswinefevervirus
AT konglingyun cloningandidentificationofpk15cellsforenhancedreplicationofclassicalswinefevervirus
AT ninghongmei cloningandidentificationofpk15cellsforenhancedreplicationofclassicalswinefevervirus
AT yuefeng cloningandidentificationofpk15cellsforenhancedreplicationofclassicalswinefevervirus
AT jiangjinqing cloningandidentificationofpk15cellsforenhancedreplicationofclassicalswinefevervirus
AT wangxuannian cloningandidentificationofpk15cellsforenhancedreplicationofclassicalswinefevervirus
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