A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood Plasma

A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood Plasma

A fluorescent probe for glutathione (GSH) detection was developed. Our study indicates a possible mechanism which couples a conjugate addition and micelle-catalyzed large membered ring formation/elimination sequence. This method enables excellent selectivity towards GSH over other biological thiols...

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Bibliographic Details
Main Authors: Robert M. Strongin, Mark Lowry, Jorge O. Escobedo, Aabha Barve, Lovemore Hakuna, Xiaofeng Yang, Yixing Guo
Format: Article
Language:English
Published: MDPI AG 2012-05-01
Series:Sensors
Subjects:
fluorescent probe
glutathione
micelle-catalyzed
selectivity
Online Access:http://www.mdpi.com/1424-8220/12/5/5940
Description
Summary:A fluorescent probe for glutathione (GSH) detection was developed. Our study indicates a possible mechanism which couples a conjugate addition and micelle-catalyzed large membered ring formation/elimination sequence. This method enables excellent selectivity towards GSH over other biological thiols such as cysteine (Cys) and homocysteine (Hcy). The proposed method is precise with a relative standard deviation (R.S.D) lower than 6% (<em>n </em>= 3) and has been successfully applied to determine GSH in human plasma with recoveries between 99.2% and 102.3%.
ISSN:1424-8220

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