A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood Plasma

A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood Plasma

A fluorescent probe for glutathione (GSH) detection was developed. Our study indicates a possible mechanism which couples a conjugate addition and micelle-catalyzed large membered ring formation/elimination sequence. This method enables excellent selectivity towards GSH over other biological thiols...

Full description

Bibliographic Details
Main Authors: Robert M. Strongin, Mark Lowry, Jorge O. Escobedo, Aabha Barve, Lovemore Hakuna, Xiaofeng Yang, Yixing Guo
Format: Article
Language:English
Published: MDPI AG 2012-05-01
Series:Sensors
Subjects:
fluorescent probe
glutathione
micelle-catalyzed
selectivity
Online Access:http://www.mdpi.com/1424-8220/12/5/5940
id doaj-ac5d036a1489477c94ff0264616c491f
record_format Article
spelling doaj-ac5d036a1489477c94ff0264616c491f2020-11-25T00:19:18ZengMDPI AGSensors1424-82202012-05-011255940595010.3390/s120505940A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood PlasmaRobert M. StronginMark LowryJorge O. EscobedoAabha BarveLovemore HakunaXiaofeng YangYixing GuoA fluorescent probe for glutathione (GSH) detection was developed. Our study indicates a possible mechanism which couples a conjugate addition and micelle-catalyzed large membered ring formation/elimination sequence. This method enables excellent selectivity towards GSH over other biological thiols such as cysteine (Cys) and homocysteine (Hcy). The proposed method is precise with a relative standard deviation (R.S.D) lower than 6% (<em>n </em>= 3) and has been successfully applied to determine GSH in human plasma with recoveries between 99.2% and 102.3%.http://www.mdpi.com/1424-8220/12/5/5940fluorescent probeglutathionemicelle-catalyzedselectivity
collection DOAJ
language English
format Article
sources DOAJ
author Robert M. Strongin
Mark Lowry
Jorge O. Escobedo
Aabha Barve
Lovemore Hakuna
Xiaofeng Yang
Yixing Guo
spellingShingle Robert M. Strongin
Mark Lowry
Jorge O. Escobedo
Aabha Barve
Lovemore Hakuna
Xiaofeng Yang
Yixing Guo
A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood Plasma
Sensors
fluorescent probe
glutathione
micelle-catalyzed
selectivity
author_facet Robert M. Strongin
Mark Lowry
Jorge O. Escobedo
Aabha Barve
Lovemore Hakuna
Xiaofeng Yang
Yixing Guo
author_sort Robert M. Strongin
title A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood Plasma
title_short A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood Plasma
title_full A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood Plasma
title_fullStr A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood Plasma
title_full_unstemmed A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood Plasma
title_sort fast response highly selective probe for the detection of glutathione in human blood plasma
publisher MDPI AG
series Sensors
issn 1424-8220
publishDate 2012-05-01
description A fluorescent probe for glutathione (GSH) detection was developed. Our study indicates a possible mechanism which couples a conjugate addition and micelle-catalyzed large membered ring formation/elimination sequence. This method enables excellent selectivity towards GSH over other biological thiols such as cysteine (Cys) and homocysteine (Hcy). The proposed method is precise with a relative standard deviation (R.S.D) lower than 6% (<em>n </em>= 3) and has been successfully applied to determine GSH in human plasma with recoveries between 99.2% and 102.3%.
topic fluorescent probe
glutathione
micelle-catalyzed
selectivity
url http://www.mdpi.com/1424-8220/12/5/5940
work_keys_str_mv AT robertmstrongin afastresponsehighlyselectiveprobeforthedetectionofglutathioneinhumanbloodplasma
AT marklowry afastresponsehighlyselectiveprobeforthedetectionofglutathioneinhumanbloodplasma
AT jorgeoescobedo afastresponsehighlyselectiveprobeforthedetectionofglutathioneinhumanbloodplasma
AT aabhabarve afastresponsehighlyselectiveprobeforthedetectionofglutathioneinhumanbloodplasma
AT lovemorehakuna afastresponsehighlyselectiveprobeforthedetectionofglutathioneinhumanbloodplasma
AT xiaofengyang afastresponsehighlyselectiveprobeforthedetectionofglutathioneinhumanbloodplasma
AT yixingguo afastresponsehighlyselectiveprobeforthedetectionofglutathioneinhumanbloodplasma
AT robertmstrongin fastresponsehighlyselectiveprobeforthedetectionofglutathioneinhumanbloodplasma
AT marklowry fastresponsehighlyselectiveprobeforthedetectionofglutathioneinhumanbloodplasma
AT jorgeoescobedo fastresponsehighlyselectiveprobeforthedetectionofglutathioneinhumanbloodplasma
AT aabhabarve fastresponsehighlyselectiveprobeforthedetectionofglutathioneinhumanbloodplasma
AT lovemorehakuna fastresponsehighlyselectiveprobeforthedetectionofglutathioneinhumanbloodplasma
AT xiaofengyang fastresponsehighlyselectiveprobeforthedetectionofglutathioneinhumanbloodplasma
AT yixingguo fastresponsehighlyselectiveprobeforthedetectionofglutathioneinhumanbloodplasma
_version_ 1725372038897467392

Similar Items