A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood Plasma
A fluorescent probe for glutathione (GSH) detection was developed. Our study indicates a possible mechanism which couples a conjugate addition and micelle-catalyzed large membered ring formation/elimination sequence. This method enables excellent selectivity towards GSH over other biological thiols...
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2012-05-01
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Online Access: | http://www.mdpi.com/1424-8220/12/5/5940 |
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doaj-ac5d036a1489477c94ff0264616c491f2020-11-25T00:19:18ZengMDPI AGSensors1424-82202012-05-011255940595010.3390/s120505940A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood PlasmaRobert M. StronginMark LowryJorge O. EscobedoAabha BarveLovemore HakunaXiaofeng YangYixing GuoA fluorescent probe for glutathione (GSH) detection was developed. Our study indicates a possible mechanism which couples a conjugate addition and micelle-catalyzed large membered ring formation/elimination sequence. This method enables excellent selectivity towards GSH over other biological thiols such as cysteine (Cys) and homocysteine (Hcy). The proposed method is precise with a relative standard deviation (R.S.D) lower than 6% (<em>n </em>= 3) and has been successfully applied to determine GSH in human plasma with recoveries between 99.2% and 102.3%.http://www.mdpi.com/1424-8220/12/5/5940fluorescent probeglutathionemicelle-catalyzedselectivity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Robert M. Strongin Mark Lowry Jorge O. Escobedo Aabha Barve Lovemore Hakuna Xiaofeng Yang Yixing Guo |
spellingShingle |
Robert M. Strongin Mark Lowry Jorge O. Escobedo Aabha Barve Lovemore Hakuna Xiaofeng Yang Yixing Guo A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood Plasma Sensors fluorescent probe glutathione micelle-catalyzed selectivity |
author_facet |
Robert M. Strongin Mark Lowry Jorge O. Escobedo Aabha Barve Lovemore Hakuna Xiaofeng Yang Yixing Guo |
author_sort |
Robert M. Strongin |
title |
A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood Plasma |
title_short |
A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood Plasma |
title_full |
A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood Plasma |
title_fullStr |
A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood Plasma |
title_full_unstemmed |
A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood Plasma |
title_sort |
fast response highly selective probe for the detection of glutathione in human blood plasma |
publisher |
MDPI AG |
series |
Sensors |
issn |
1424-8220 |
publishDate |
2012-05-01 |
description |
A fluorescent probe for glutathione (GSH) detection was developed. Our study indicates a possible mechanism which couples a conjugate addition and micelle-catalyzed large membered ring formation/elimination sequence. This method enables excellent selectivity towards GSH over other biological thiols such as cysteine (Cys) and homocysteine (Hcy). The proposed method is precise with a relative standard deviation (R.S.D) lower than 6% (<em>n </em>= 3) and has been successfully applied to determine GSH in human plasma with recoveries between 99.2% and 102.3%. |
topic |
fluorescent probe glutathione micelle-catalyzed selectivity |
url |
http://www.mdpi.com/1424-8220/12/5/5940 |
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