Evaluating the effects of different fractions obtained from Gundelia tournefortii extract against carbon tetrachloride-induced liver injury in rats
<p>Xenobiotics-induced liver injury is a major challenge for clinicians and pharmaceutical industry. Hence, finding new therapeutic molecules against this complication has clinical value. The current investigation aimed to evaluate the potential protective effects of different fractions obtain...
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doaj-ac60e273c939470488a60a6f3d5912682020-11-25T01:49:59ZengShiraz University of Medical SciencesTrends in Pharmaceutical Sciences2423-37222423-56522016-02-0121253410.1111/tips.v2i1.5840Evaluating the effects of different fractions obtained from Gundelia tournefortii extract against carbon tetrachloride-induced liver injury in ratsHossein Niknahad0Reza Heidari1Tannaz Mokhtebaz2Sasan Mansouri3Shadab Dehshahri4Narges Abdoli5Asma Najibi6Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. & Department of Pharmacology and Toxicology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, IranShiraz University of Medical Sciences, Pharmaceutical Sciences Research CenterStudent Research Committee, International branch, Shiraz University of Medical Sciences, Shiraz, IranStudent Research Committee, International branch, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Pharmacognosy, School of Pharmacy, Shiraz University of Medical Sciences, International Branch, Shiraz, IranFood and Drug Organization, Ministry of Health, Tehran, IranDepartment of Pharmacology and Toxicology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran<p>Xenobiotics-induced liver injury is a major challenge for clinicians and pharmaceutical industry. Hence, finding new therapeutic molecules against this complication has clinical value. The current investigation aimed to evaluate the potential protective effects of different fractions obtained from <em>Gundelia tournefortii </em>(GT)<em> </em>hydroalcoholic extract in a rat model of acute hepatic injury. Male Sprague-Dawley rats (200‑250 g) were treated with carbon tetrachloride (CCl<sub>4</sub>) (1.5 ml/kg, i.p), then ethanol, water, chloroform, ethyl acetate, and n-Butanol fractions of GT<em> </em>extract were administered. Biochemical and histopathological markers of hepatic injury were assessed and glutathione (GSH) and lipid peroxidation were monitored in liver samples. CCl<sub>4</sub> administration caused hepatotoxicity as revealed by an increase in serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) activity, as well as pathological changes of the liver. Furthermore, a significant reduction in hepatic glutathione content and an elevation in lipid peroxidation were observed in CCl<sub>4</sub>‑treated rats. It was found that the n‑butanol (200 mg/kg) and the ethyl acetate (300 mg/kg) fractions of GT extract protected liver against CCL<sub>4</sub>‑induced damage as judged by lower AST, ALT, LDH and lipid peroxidation, prevention of tissue glutathione depletion, and alleviation of histopathological damages of liver in extract‑treated animals. As n‑butanol and the ethyl acetate fractions of GT<em> </em>effectively alleviated the liver injury induced by CCl<sub>4</sub> and provide antioxidant properties, we might be able to propose that the hepatoprotective chemicals of <em>Gundelia </em>extract are present in these fractions.</p>http://tips.sums.ac.ir/index.php/TiPS/article/view/58Gundelia tournefortii, Hepatoprotective, Hepatotoxicity, Liver injury, Medicinal plants |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hossein Niknahad Reza Heidari Tannaz Mokhtebaz Sasan Mansouri Shadab Dehshahri Narges Abdoli Asma Najibi |
spellingShingle |
Hossein Niknahad Reza Heidari Tannaz Mokhtebaz Sasan Mansouri Shadab Dehshahri Narges Abdoli Asma Najibi Evaluating the effects of different fractions obtained from Gundelia tournefortii extract against carbon tetrachloride-induced liver injury in rats Trends in Pharmaceutical Sciences Gundelia tournefortii, Hepatoprotective, Hepatotoxicity, Liver injury, Medicinal plants |
author_facet |
Hossein Niknahad Reza Heidari Tannaz Mokhtebaz Sasan Mansouri Shadab Dehshahri Narges Abdoli Asma Najibi |
author_sort |
Hossein Niknahad |
title |
Evaluating the effects of different fractions obtained from Gundelia tournefortii extract against carbon tetrachloride-induced liver injury in rats |
title_short |
Evaluating the effects of different fractions obtained from Gundelia tournefortii extract against carbon tetrachloride-induced liver injury in rats |
title_full |
Evaluating the effects of different fractions obtained from Gundelia tournefortii extract against carbon tetrachloride-induced liver injury in rats |
title_fullStr |
Evaluating the effects of different fractions obtained from Gundelia tournefortii extract against carbon tetrachloride-induced liver injury in rats |
title_full_unstemmed |
Evaluating the effects of different fractions obtained from Gundelia tournefortii extract against carbon tetrachloride-induced liver injury in rats |
title_sort |
evaluating the effects of different fractions obtained from gundelia tournefortii extract against carbon tetrachloride-induced liver injury in rats |
publisher |
Shiraz University of Medical Sciences |
series |
Trends in Pharmaceutical Sciences |
issn |
2423-3722 2423-5652 |
publishDate |
2016-02-01 |
description |
<p>Xenobiotics-induced liver injury is a major challenge for clinicians and pharmaceutical industry. Hence, finding new therapeutic molecules against this complication has clinical value. The current investigation aimed to evaluate the potential protective effects of different fractions obtained from <em>Gundelia tournefortii </em>(GT)<em> </em>hydroalcoholic extract in a rat model of acute hepatic injury. Male Sprague-Dawley rats (200‑250 g) were treated with carbon tetrachloride (CCl<sub>4</sub>) (1.5 ml/kg, i.p), then ethanol, water, chloroform, ethyl acetate, and n-Butanol fractions of GT<em> </em>extract were administered. Biochemical and histopathological markers of hepatic injury were assessed and glutathione (GSH) and lipid peroxidation were monitored in liver samples. CCl<sub>4</sub> administration caused hepatotoxicity as revealed by an increase in serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) activity, as well as pathological changes of the liver. Furthermore, a significant reduction in hepatic glutathione content and an elevation in lipid peroxidation were observed in CCl<sub>4</sub>‑treated rats. It was found that the n‑butanol (200 mg/kg) and the ethyl acetate (300 mg/kg) fractions of GT extract protected liver against CCL<sub>4</sub>‑induced damage as judged by lower AST, ALT, LDH and lipid peroxidation, prevention of tissue glutathione depletion, and alleviation of histopathological damages of liver in extract‑treated animals. As n‑butanol and the ethyl acetate fractions of GT<em> </em>effectively alleviated the liver injury induced by CCl<sub>4</sub> and provide antioxidant properties, we might be able to propose that the hepatoprotective chemicals of <em>Gundelia </em>extract are present in these fractions.</p> |
topic |
Gundelia tournefortii, Hepatoprotective, Hepatotoxicity, Liver injury, Medicinal plants |
url |
http://tips.sums.ac.ir/index.php/TiPS/article/view/58 |
work_keys_str_mv |
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