Rapid Lentiviral Vector Producer Cell Line Generation Using a Single DNA Construct

Rapid Lentiviral Vector Producer Cell Line Generation Using a Single DNA Construct

Stable suspension producer cell lines for the production of vesicular stomatitis virus envelope glycoprotein (VSVg)-pseudotyped lentiviral vectors represent an attractive alternative to current widely used production methods based on transient transfection of adherent 293T cells with multiple plasmi...

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Bibliographic Details
Main Authors: Yu Hua Chen, Celeste Pallant, Christopher J. Sampson, Alessia Boiti, Sabine Johnson, Pijus Brazauskas, Philip Hardwicke, Michela Marongiu, Vanesa M. Marinova, Marlene Carmo, Nathan P. Sweeney, Ashkenaz Richard, Anthony Shillings, Peter Archibald, Eva Puschmann, Bernadette Mouzon, David Grose, Miriam Mendez-Tavio, Mao Xiang Chen, Stephen R.C. Warr, Tarik Senussi, Paul S. Carter, Sean Baker, Cindy Jung, Martijn H. Brugman, Steven J. Howe, Conrad A. Vink
Format: Article
Language:English
Published: Elsevier 2020-12-01
Series:Molecular Therapy: Methods & Clinical Development
Subjects:
lentiviral vector
cell line development
producer cell line
manufacturing
Online Access:http://www.sciencedirect.com/science/article/pii/S2329050120301777

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http://www.sciencedirect.com/science/article/pii/S2329050120301777

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