Viability of bacteria in dental calculus - A microbiological study
<b>Aim:</b> The aim of this study was (1) To investigate the viability of bacteria within supragingival and subgingival calculus, (2) To examine motility of bacteria, and (3) To identify bacterial morphotypes in calculus. <b>Materials and Methods:</b> Supra and subgingival ca...
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Wolters Kluwer Medknow Publications
2010-01-01
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doaj-aca4df3ec50b4f2c8903545e39c499ab2020-11-24T23:48:29ZengWolters Kluwer Medknow PublicationsJournal of Indian Society of Periodontology0972-124X0975-15802010-01-01144222226Viability of bacteria in dental calculus - A microbiological studyMoolya NikeshThakur SrinathRavindra SSetty SwatiKulkarni RaghavendraHallikeri Kaveri<b>Aim:</b> The aim of this study was (1) To investigate the viability of bacteria within supragingival and subgingival calculus, (2) To examine motility of bacteria, and (3) To identify bacterial morphotypes in calculus. <b>Materials and Methods:</b> Supra and subgingival calculus were harvested from 30 subjects having clinical evidence of chronic inflammatory periodontal disease and were divided into two groups <b>.</b> Samples from both groups were immediately transported to the Department of Microbiology for gram staining, acridine orange staining, bacterial culture and to the Department of Oral Pathology for dark field microscopy. <b>Results:</b> Gram staining revealed presence of bacteria within the samples. Dark field microscopic examination revealed presence of filamentous organisms, spirochetes, and motile short bacilli. Acridine orange fluorescent stain showed that the viable bacteria appeared apple green. Bacterial culture revealed presence of a variety of aerobic organisms. <b>Conclusion:</b> From the results, it appeared that viable bacteria were present within calculus especially within internal channels and lacunae.http://www.jisponline.com/article.asp?issn=0972-124X;year=2010;volume=14;issue=4;spage=222;epage=226;aulast=MoolyaAcridine orange stainingaerobic bacterial culturegram stainingspirochetessubgingival calculus |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Moolya Nikesh Thakur Srinath Ravindra S Setty Swati Kulkarni Raghavendra Hallikeri Kaveri |
spellingShingle |
Moolya Nikesh Thakur Srinath Ravindra S Setty Swati Kulkarni Raghavendra Hallikeri Kaveri Viability of bacteria in dental calculus - A microbiological study Journal of Indian Society of Periodontology Acridine orange staining aerobic bacterial culture gram staining spirochetes subgingival calculus |
author_facet |
Moolya Nikesh Thakur Srinath Ravindra S Setty Swati Kulkarni Raghavendra Hallikeri Kaveri |
author_sort |
Moolya Nikesh |
title |
Viability of bacteria in dental calculus - A microbiological study |
title_short |
Viability of bacteria in dental calculus - A microbiological study |
title_full |
Viability of bacteria in dental calculus - A microbiological study |
title_fullStr |
Viability of bacteria in dental calculus - A microbiological study |
title_full_unstemmed |
Viability of bacteria in dental calculus - A microbiological study |
title_sort |
viability of bacteria in dental calculus - a microbiological study |
publisher |
Wolters Kluwer Medknow Publications |
series |
Journal of Indian Society of Periodontology |
issn |
0972-124X 0975-1580 |
publishDate |
2010-01-01 |
description |
<b>Aim:</b> The aim of this study was (1) To investigate the viability of bacteria within supragingival and subgingival calculus, (2) To examine motility of bacteria, and (3) To identify bacterial morphotypes in calculus. <b>Materials and Methods:</b> Supra and subgingival calculus were harvested from 30 subjects having clinical evidence of chronic inflammatory periodontal disease and were divided into two groups <b>.</b> Samples from both groups were immediately transported to the Department of Microbiology for gram staining, acridine orange staining, bacterial culture and to the Department of Oral Pathology for dark field microscopy. <b>Results:</b> Gram staining revealed presence of bacteria within the samples. Dark field microscopic examination revealed presence of filamentous organisms, spirochetes, and motile short bacilli. Acridine orange fluorescent stain showed that the viable bacteria appeared apple green. Bacterial culture revealed presence of a variety of aerobic organisms. <b>Conclusion:</b> From the results, it appeared that viable bacteria were present within calculus especially within internal channels and lacunae. |
topic |
Acridine orange staining aerobic bacterial culture gram staining spirochetes subgingival calculus |
url |
http://www.jisponline.com/article.asp?issn=0972-124X;year=2010;volume=14;issue=4;spage=222;epage=226;aulast=Moolya |
work_keys_str_mv |
AT moolyanikesh viabilityofbacteriaindentalcalculusamicrobiologicalstudy AT thakursrinath viabilityofbacteriaindentalcalculusamicrobiologicalstudy AT ravindras viabilityofbacteriaindentalcalculusamicrobiologicalstudy AT settyswati viabilityofbacteriaindentalcalculusamicrobiologicalstudy AT kulkarniraghavendra viabilityofbacteriaindentalcalculusamicrobiologicalstudy AT hallikerikaveri viabilityofbacteriaindentalcalculusamicrobiologicalstudy |
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