The distribution and physiological effects of the myoinhibiting peptides in the kissing bug, Rhodnius prolixus
The myoinhibiting peptides (MIPs), also designated as allatostatin-Bs (AST-Bs) or prothoracicostatic peptides (PTSPs) in some insects, are neuropeptides that are characterized by two tryptophan (W) residues at the C-terminal, denoted as the W(X6)Wamide motif. They are believed to be the ancestral li...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2012-07-01
|
Series: | Frontiers in Neuroscience |
Subjects: | |
Online Access: | http://journal.frontiersin.org/Journal/10.3389/fnins.2012.00098/full |
id |
doaj-aca4df41d0e5472f9a07139480c85eff |
---|---|
record_format |
Article |
spelling |
doaj-aca4df41d0e5472f9a07139480c85eff2020-11-24T22:53:21ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2012-07-01610.3389/fnins.2012.0009827015The distribution and physiological effects of the myoinhibiting peptides in the kissing bug, Rhodnius prolixusAngela eLange0Uzma eAlim1Hans Peter eVandersmissen2Akira eMizoguchi3Jozef eVanden Broeck4Ian eOrchard5University of Toronto MississaugaUniversity of Toronto MississaugaKU LeuvenNagoya UniversityKU LeuvenUniversity of Toronto MississaugaThe myoinhibiting peptides (MIPs), also designated as allatostatin-Bs (AST-Bs) or prothoracicostatic peptides (PTSPs) in some insects, are neuropeptides that are characterized by two tryptophan (W) residues at the C-terminal, denoted as the W(X6)Wamide motif. They are believed to be the ancestral ligands for the Drosophila sex peptide (SP) receptor. Physiological functions of MIPs include the inhibition of contraction of insect visceral muscles, in addition to allatostatic and prothoracicostatic activities. The MIP precursor in Rhodnius prolixus encodes MIPs that have an unusual W(X7)Wamide motif. In the present study, MIP-like immunoreactivity was detected within neurons in the central nervous system and within the innervation to the salivary glands, hindgut, and female and male reproductive systems of adult R. prolixus. The effects of peptides with the unusual W(X7)Wamide motif (Rhopr-MIP-4) and with the typical W(X6)Wamide motif (Rhopr-MIP-7) were tested for physiological activity on R. prolixus hindgut contractions. Both peptides reduce the frequency and amplitude of hindgut contractions in a dose-dependent manner. In addition, both peptides activate the Drosophila sex peptide receptor. The MIP/SP receptors are therefore activated by peptides with the unusual W(X7)Wamide motif.http://journal.frontiersin.org/Journal/10.3389/fnins.2012.00098/fullImmunohistochemistryMuscle Contractioninsectreceptor assayreproductive tissuessalivary gland |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Angela eLange Uzma eAlim Hans Peter eVandersmissen Akira eMizoguchi Jozef eVanden Broeck Ian eOrchard |
spellingShingle |
Angela eLange Uzma eAlim Hans Peter eVandersmissen Akira eMizoguchi Jozef eVanden Broeck Ian eOrchard The distribution and physiological effects of the myoinhibiting peptides in the kissing bug, Rhodnius prolixus Frontiers in Neuroscience Immunohistochemistry Muscle Contraction insect receptor assay reproductive tissues salivary gland |
author_facet |
Angela eLange Uzma eAlim Hans Peter eVandersmissen Akira eMizoguchi Jozef eVanden Broeck Ian eOrchard |
author_sort |
Angela eLange |
title |
The distribution and physiological effects of the myoinhibiting peptides in the kissing bug, Rhodnius prolixus |
title_short |
The distribution and physiological effects of the myoinhibiting peptides in the kissing bug, Rhodnius prolixus |
title_full |
The distribution and physiological effects of the myoinhibiting peptides in the kissing bug, Rhodnius prolixus |
title_fullStr |
The distribution and physiological effects of the myoinhibiting peptides in the kissing bug, Rhodnius prolixus |
title_full_unstemmed |
The distribution and physiological effects of the myoinhibiting peptides in the kissing bug, Rhodnius prolixus |
title_sort |
distribution and physiological effects of the myoinhibiting peptides in the kissing bug, rhodnius prolixus |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Neuroscience |
issn |
1662-453X |
publishDate |
2012-07-01 |
description |
The myoinhibiting peptides (MIPs), also designated as allatostatin-Bs (AST-Bs) or prothoracicostatic peptides (PTSPs) in some insects, are neuropeptides that are characterized by two tryptophan (W) residues at the C-terminal, denoted as the W(X6)Wamide motif. They are believed to be the ancestral ligands for the Drosophila sex peptide (SP) receptor. Physiological functions of MIPs include the inhibition of contraction of insect visceral muscles, in addition to allatostatic and prothoracicostatic activities. The MIP precursor in Rhodnius prolixus encodes MIPs that have an unusual W(X7)Wamide motif. In the present study, MIP-like immunoreactivity was detected within neurons in the central nervous system and within the innervation to the salivary glands, hindgut, and female and male reproductive systems of adult R. prolixus. The effects of peptides with the unusual W(X7)Wamide motif (Rhopr-MIP-4) and with the typical W(X6)Wamide motif (Rhopr-MIP-7) were tested for physiological activity on R. prolixus hindgut contractions. Both peptides reduce the frequency and amplitude of hindgut contractions in a dose-dependent manner. In addition, both peptides activate the Drosophila sex peptide receptor. The MIP/SP receptors are therefore activated by peptides with the unusual W(X7)Wamide motif. |
topic |
Immunohistochemistry Muscle Contraction insect receptor assay reproductive tissues salivary gland |
url |
http://journal.frontiersin.org/Journal/10.3389/fnins.2012.00098/full |
work_keys_str_mv |
AT angelaelange thedistributionandphysiologicaleffectsofthemyoinhibitingpeptidesinthekissingbugrhodniusprolixus AT uzmaealim thedistributionandphysiologicaleffectsofthemyoinhibitingpeptidesinthekissingbugrhodniusprolixus AT hanspeterevandersmissen thedistributionandphysiologicaleffectsofthemyoinhibitingpeptidesinthekissingbugrhodniusprolixus AT akiraemizoguchi thedistributionandphysiologicaleffectsofthemyoinhibitingpeptidesinthekissingbugrhodniusprolixus AT jozefevandenbroeck thedistributionandphysiologicaleffectsofthemyoinhibitingpeptidesinthekissingbugrhodniusprolixus AT ianeorchard thedistributionandphysiologicaleffectsofthemyoinhibitingpeptidesinthekissingbugrhodniusprolixus AT angelaelange distributionandphysiologicaleffectsofthemyoinhibitingpeptidesinthekissingbugrhodniusprolixus AT uzmaealim distributionandphysiologicaleffectsofthemyoinhibitingpeptidesinthekissingbugrhodniusprolixus AT hanspeterevandersmissen distributionandphysiologicaleffectsofthemyoinhibitingpeptidesinthekissingbugrhodniusprolixus AT akiraemizoguchi distributionandphysiologicaleffectsofthemyoinhibitingpeptidesinthekissingbugrhodniusprolixus AT jozefevandenbroeck distributionandphysiologicaleffectsofthemyoinhibitingpeptidesinthekissingbugrhodniusprolixus AT ianeorchard distributionandphysiologicaleffectsofthemyoinhibitingpeptidesinthekissingbugrhodniusprolixus |
_version_ |
1725663716338302976 |