Silver nanoparticles inhibit beige fat function and promote adiposity
Objective: Obesity is a complex chronic disease of high prevalence worldwide. Multiple factors play integral roles in obesity development, with rising interest focusing on the contribution of environmental pollutants frequent in modern society. Silver nanoparticles (AgNPs) are widely used for bacter...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-04-01
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| Series: | Molecular Metabolism |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212877818310081 |
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Article |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Lishu Yue Wenjun Zhao Dongmei Wang Meiyao Meng Ying Zheng Yu Li Jin Qiu Jian Yu Yang Yan Peng Lu Youmin Sun Jie Fu Jiqiu Wang Qiang Zhang Lingyan Xu Xinran Ma |
| spellingShingle |
Lishu Yue Wenjun Zhao Dongmei Wang Meiyao Meng Ying Zheng Yu Li Jin Qiu Jian Yu Yang Yan Peng Lu Youmin Sun Jie Fu Jiqiu Wang Qiang Zhang Lingyan Xu Xinran Ma Silver nanoparticles inhibit beige fat function and promote adiposity Molecular Metabolism |
| author_facet |
Lishu Yue Wenjun Zhao Dongmei Wang Meiyao Meng Ying Zheng Yu Li Jin Qiu Jian Yu Yang Yan Peng Lu Youmin Sun Jie Fu Jiqiu Wang Qiang Zhang Lingyan Xu Xinran Ma |
| author_sort |
Lishu Yue |
| title |
Silver nanoparticles inhibit beige fat function and promote adiposity |
| title_short |
Silver nanoparticles inhibit beige fat function and promote adiposity |
| title_full |
Silver nanoparticles inhibit beige fat function and promote adiposity |
| title_fullStr |
Silver nanoparticles inhibit beige fat function and promote adiposity |
| title_full_unstemmed |
Silver nanoparticles inhibit beige fat function and promote adiposity |
| title_sort |
silver nanoparticles inhibit beige fat function and promote adiposity |
| publisher |
Elsevier |
| series |
Molecular Metabolism |
| issn |
2212-8778 |
| publishDate |
2019-04-01 |
| description |
Objective: Obesity is a complex chronic disease of high prevalence worldwide. Multiple factors play integral roles in obesity development, with rising interest focusing on the contribution of environmental pollutants frequent in modern society. Silver nanoparticles (AgNPs) are widely used for bactericidal purpose in various applications in daily life. However, their potential toxicity and contribution to the obesity epidemic are not clear. Methods: Beige adipocytes are newly discovered adipocytes characterized by high thermogenic and energy dissipating capacity upon activation and the “browning” process. In the present study, we assess the impact of AgNPs exposure on beige adipocytes differentiation and functionality both in vitro and in vivo. We also systematically investigate the influence of AgNPs on adiposity and metabolic performance in mice, as well as the possible underlying molecular mechanism. Results: The results showed that, independent of particle size, AgNPs inhibit the adipogenic, mitochondrial, and thermogenic gene programs of beige adipocytes, thus suppressing their differentiation ability, mitochondrial activity, and thermogenic response. Importantly, exposure to AgNPs in mice suppresses browning gene programs in subcutaneous fat, leading to decreased energy expenditure and increased adiposity in mice. Mechanistically, we found that AgNPs increase reactive oxidative species (ROS) levels and specifically activate MAPK-ERK signaling in beige adipocytes. The negative impacts of AgNPs on beige adipocytes can be ameliorated by antioxidant or ERK inhibitor FR180204 treatment. Conclusions: Taken together, these results revealed an unexpected role of AgNPs in promoting adiposity through the inhibition of beige adipocyte differentiation and functionality, possibly by disrupting ROS homeostasis and ERK phosphorylation. Future assessments on the health risk of AgNPs applications and their safe dosages are warranted. Keywords: Adiposity, Beige fat, Silver nanoparticles, Browning, Energy expenditure, Reactive oxidative species |
| url |
http://www.sciencedirect.com/science/article/pii/S2212877818310081 |
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doaj-acca582a294547baa308eacf17602b592020-11-24T23:59:28ZengElsevierMolecular Metabolism2212-87782019-04-0122111Silver nanoparticles inhibit beige fat function and promote adiposityLishu Yue0Wenjun Zhao1Dongmei Wang2Meiyao Meng3Ying Zheng4Yu Li5Jin Qiu6Jian Yu7Yang Yan8Peng Lu9Youmin Sun10Jie Fu11Jiqiu Wang12Qiang Zhang13Lingyan Xu14Xinran Ma15Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, 200241, ChinaShanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, 200241, ChinaShanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, 200241, ChinaShanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, 200241, ChinaShanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, 200241, ChinaShanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, 200241, ChinaShanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, 200241, ChinaShanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, 200241, ChinaShanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, 200241, ChinaDepartment of Endocrinology and Metabolism, China National Research Center for Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, ChinaDepartment of Environmental Science & Engineering, Fudan University, Shanghai, 200433, ChinaDepartment of Environmental Science & Engineering, Fudan University, Shanghai, 200433, ChinaDepartment of Endocrinology and Metabolism, China National Research Center for Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, ChinaShanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, 200241, ChinaShanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, 200241, China; Corresponding author.Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, 200241, China; Corresponding author.Objective: Obesity is a complex chronic disease of high prevalence worldwide. Multiple factors play integral roles in obesity development, with rising interest focusing on the contribution of environmental pollutants frequent in modern society. Silver nanoparticles (AgNPs) are widely used for bactericidal purpose in various applications in daily life. However, their potential toxicity and contribution to the obesity epidemic are not clear. Methods: Beige adipocytes are newly discovered adipocytes characterized by high thermogenic and energy dissipating capacity upon activation and the “browning” process. In the present study, we assess the impact of AgNPs exposure on beige adipocytes differentiation and functionality both in vitro and in vivo. We also systematically investigate the influence of AgNPs on adiposity and metabolic performance in mice, as well as the possible underlying molecular mechanism. Results: The results showed that, independent of particle size, AgNPs inhibit the adipogenic, mitochondrial, and thermogenic gene programs of beige adipocytes, thus suppressing their differentiation ability, mitochondrial activity, and thermogenic response. Importantly, exposure to AgNPs in mice suppresses browning gene programs in subcutaneous fat, leading to decreased energy expenditure and increased adiposity in mice. Mechanistically, we found that AgNPs increase reactive oxidative species (ROS) levels and specifically activate MAPK-ERK signaling in beige adipocytes. The negative impacts of AgNPs on beige adipocytes can be ameliorated by antioxidant or ERK inhibitor FR180204 treatment. Conclusions: Taken together, these results revealed an unexpected role of AgNPs in promoting adiposity through the inhibition of beige adipocyte differentiation and functionality, possibly by disrupting ROS homeostasis and ERK phosphorylation. Future assessments on the health risk of AgNPs applications and their safe dosages are warranted. Keywords: Adiposity, Beige fat, Silver nanoparticles, Browning, Energy expenditure, Reactive oxidative specieshttp://www.sciencedirect.com/science/article/pii/S2212877818310081 |