| Summary: | In this work, Pani and Pani@g-C<sub>3</sub>N<sub>4</sub> was synthesized by in situ oxidative polymerization methodology of aniline, in the presence of g-C<sub>3</sub>N<sub>4</sub>. The as prepared Pani@g-C<sub>3</sub>N<sub>4</sub> was characterized by scanning electron microscopy, transmission electron microscopy and X-ray diffraction (XRD). The morphological analysis showed well dispersed Pani in g-C<sub>3</sub>N<sub>4</sub>, as well as the coating of Pani on g-C<sub>3</sub>N<sub>4</sub>. The XRD further revealed this, and peaks of Pani as well as g-C<sub>3</sub>N<sub>4</sub> was observed, thereby suggesting successful synthesis of the composite. The DC electrical conductivity studies under isothermal and cyclic aging conditions showed high stability of composites over 100 °C. Further, the synthesized composite material proved to be an excellent antimicrobial agent against both type i.e., gram positive <i>Streptococcus pneumoniae</i> and negative bacteria <i>Escherichia coli</i>. In the zone inhibition assay 18 ± 0.5, 16 ± 0.75 and 20 ± 0.5, 22 ± 0.5 mm zone diameter were found against <i>E. coli</i> and <i>S. pneumoniae</i> in presence of pure g-C<sub>3</sub>N<sub>4</sub> and Pani@g-C<sub>3</sub>N<sub>4</sub> at 50 µg concentrations, respectively. Further antimicrobial activity in the presence of sunlight in aqueous medium showed that Pani@g-C<sub>3</sub>N<sub>4</sub> is more potent than pure g-C<sub>3</sub>N<sub>4</sub>.
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