INHIBITION OF GROWTH OF BACTERIA IN STAPHYLOCOCCUS AUREUS AND PSEUDOMONAS AERUGINOSA CULTURES BY COPPER AND ZINC CATIONS, APPLIED AT PHYSIOLOGICAL CONCENTRATIONS

• Main Authors: S. B. Cheknev, E. I. Vostrova, M. A. Sarycheva, A. V. Vostrov
• Format: Article
• Language: Russian
• Published: Central Research Institute for Epidemiology 2016-06-01
• Series: Журнал микробиологии, эпидемиологии и иммунобиологии
• Subjects: s.aureus; p.aeruginosa; antibacterial effect; copper; zinc; s. aureus; p. aeruginosa
• Online Access: https://microbiol.elpub.ru/jour/article/view/43
• ISSN: 0372-9311; 2686-7613

Aim. Evaluation of antibacterial effect of y-globulin fraction bound and free copper and zinc cations, applied in cultures of S. aureus and P. aeruginosa at physiological (micromolar) concentrations. Materials and methods. Day cultures of S. aureus or P. aeruginosa were transferred from agar to physiological solution, and cell suspension was prepared, containing approximately 103 - 104 CFU/ml. Samples of metal-complexes of y-globulin with copper and zinc cations (30 and 45 pg/ml), control y-globulins (30 and 45 pg/ml) and salt solutions of copper and zinc, cation content in those corresponded to the quantity of the metal, that had bound with the protein at the stage of metal-complex obtaining (75 ng/ml), were introduced into the suspension. The suspensions were incubated at 37°C for 6 hours, sampling and CFU count according to the accepted micromethod was carried out every 2 hours. By the end of incubation (6 hours of observation) the suspensions were transferred into nutrient broth, thermostated for 1 day at 37°C, transparency of the nutrient broth compared with control (sterile) was evaluated afterwards. Results. Toxic effect of copper and zinc cations is detected starting from the 3rd hour of observation in S. aureus culture. Viable bacteria are absent in the culture with zinc after 6 hours, with copper - after 4 hours of incubation, y-globulin, that had bound copper cations, reduces the quantity of viable cells compared with control protein by 11.9 - 33.0% (p