ggsashimi: Sashimi plot revised for browser- and annotation-independent splicing visualization.

We present ggsashimi, a command-line tool for the visualization of splicing events across multiple samples. Given a specified genomic region, ggsashimi creates sashimi plots for individual RNA-seq experiments as well as aggregated plots for groups of experiments, a feature unique to this software. C...

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Bibliographic Details
Main Authors: Diego Garrido-Martín, Emilio Palumbo, Roderic Guigó, Alessandra Breschi
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2018-08-01
Series:PLoS Computational Biology
Online Access:http://europepmc.org/articles/PMC6114895?pdf=render
Description
Summary:We present ggsashimi, a command-line tool for the visualization of splicing events across multiple samples. Given a specified genomic region, ggsashimi creates sashimi plots for individual RNA-seq experiments as well as aggregated plots for groups of experiments, a feature unique to this software. Compared to the existing versions of programs generating sashimi plots, it uses popular bioinformatics file formats, it is annotation-independent, and allows the visualization of splicing events even for large genomic regions by scaling down the genomic segments between splice sites. ggsashimi is freely available at https://github.com/guigolab/ggsashimi. It is implemented in python, and internally generates R code for plotting.
ISSN:1553-734X
1553-7358