Confocal spectral microscopy, a non-destructive approach to follow contamination and biofilm formation of mCherry Staphylococcus aureus on solid surfaces

Abstract Methods to test the safety of wood material for hygienically sensitive places are indirect, destructive and limited to incomplete microbial recovery via swabbing, brushing and elution-based techniques. Therefore, we chose mCherry Staphylococcus aureus as a model bacterium for solid and poro...

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Main Authors: Muhammad Tanveer Munir, Nattar Maneewan, Julien Pichon, Mohammed Gharbia, Ismael Oumarou-Mahamane, Jessica Baude, Chantal Thorin, Didier Lepelletier, Patrice Le Pape, Matthieu Eveillard, Mark Irle, Hélène Pailhoriès, Florence Aviat, Christophe Belloncle, Michel Federighi, Laurence Dubreil
Format: Article
Language:English
Published: Nature Publishing Group 2021-08-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-94939-2
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spelling doaj-ad07450ce7e747b1a16fc8a565e8bf6e2021-08-08T11:25:39ZengNature Publishing GroupScientific Reports2045-23222021-08-0111111110.1038/s41598-021-94939-2Confocal spectral microscopy, a non-destructive approach to follow contamination and biofilm formation of mCherry Staphylococcus aureus on solid surfacesMuhammad Tanveer Munir0Nattar Maneewan1Julien Pichon2Mohammed Gharbia3Ismael Oumarou-Mahamane4Jessica Baude5Chantal Thorin6Didier Lepelletier7Patrice Le Pape8Matthieu Eveillard9Mark Irle10Hélène Pailhoriès11Florence Aviat12Christophe Belloncle13Michel Federighi14Laurence Dubreil15LIMBHA, Ecole Supérieure du BoisLIMBHA, Ecole Supérieure du BoisUMR703 PAnTher APEX, INRAE/ONIRIS - La ChantrerieUMR INRAE 1014 SECALIM, OnirisLIMBHA, Ecole Supérieure du BoisCIRI, Inserm U1111, Lyon 1 University, ENS Lyon, CNRS UMR 5308NP3 Laboratory, OnirisMiHAR EE 1701 S, IRS 2, University of NantesEA 1155 IICiMed, IRS 2, University of NantesCRCINA, Inserm, University of Nantes, University of AngersLIMBHA, Ecole Supérieure du BoisLaboratory of Bacteriology-Hygiene, University Hospital of AngersYour ResearcH-Bio-ScientificLIMBHA, Ecole Supérieure du BoisUMR INRAE 1014 SECALIM, OnirisUMR703 PAnTher APEX, INRAE/ONIRIS - La ChantrerieAbstract Methods to test the safety of wood material for hygienically sensitive places are indirect, destructive and limited to incomplete microbial recovery via swabbing, brushing and elution-based techniques. Therefore, we chose mCherry Staphylococcus aureus as a model bacterium for solid and porous surface contamination. Confocal spectral laser microscope (CSLM) was employed to characterize and use the autofluorescence of Sessile oak (Quercus petraea), Douglas fir (Pseudotsuga menziesii) and poplar (Populus euramericana alba L.) wood discs cut into transversal (RT) and tangential (LT) planes. The red fluorescent area occupied by bacteria was differentiated from that of wood, which represented the bacterial quantification, survival and bio-distribution on surfaces from one hour to one week after inoculation. More bacteria were present near the surface on LT face wood as compared to RT and they persisted throughout the study period. Furthermore, this innovative methodology identified that S. aureus formed a dense biofilm on melamine but not on oak wood in similar inoculation and growth conditions. Conclusively, the endogenous fluorescence of materials and the model bacterium permitted direct quantification of surface contamination by using CSLM and it is a promising tool for hygienic safety evaluation.https://doi.org/10.1038/s41598-021-94939-2
collection DOAJ
language English
format Article
sources DOAJ
author Muhammad Tanveer Munir
Nattar Maneewan
Julien Pichon
Mohammed Gharbia
Ismael Oumarou-Mahamane
Jessica Baude
Chantal Thorin
Didier Lepelletier
Patrice Le Pape
Matthieu Eveillard
Mark Irle
Hélène Pailhoriès
Florence Aviat
Christophe Belloncle
Michel Federighi
Laurence Dubreil
spellingShingle Muhammad Tanveer Munir
Nattar Maneewan
Julien Pichon
Mohammed Gharbia
Ismael Oumarou-Mahamane
Jessica Baude
Chantal Thorin
Didier Lepelletier
Patrice Le Pape
Matthieu Eveillard
Mark Irle
Hélène Pailhoriès
Florence Aviat
Christophe Belloncle
Michel Federighi
Laurence Dubreil
Confocal spectral microscopy, a non-destructive approach to follow contamination and biofilm formation of mCherry Staphylococcus aureus on solid surfaces
Scientific Reports
author_facet Muhammad Tanveer Munir
Nattar Maneewan
Julien Pichon
Mohammed Gharbia
Ismael Oumarou-Mahamane
Jessica Baude
Chantal Thorin
Didier Lepelletier
Patrice Le Pape
Matthieu Eveillard
Mark Irle
Hélène Pailhoriès
Florence Aviat
Christophe Belloncle
Michel Federighi
Laurence Dubreil
author_sort Muhammad Tanveer Munir
title Confocal spectral microscopy, a non-destructive approach to follow contamination and biofilm formation of mCherry Staphylococcus aureus on solid surfaces
title_short Confocal spectral microscopy, a non-destructive approach to follow contamination and biofilm formation of mCherry Staphylococcus aureus on solid surfaces
title_full Confocal spectral microscopy, a non-destructive approach to follow contamination and biofilm formation of mCherry Staphylococcus aureus on solid surfaces
title_fullStr Confocal spectral microscopy, a non-destructive approach to follow contamination and biofilm formation of mCherry Staphylococcus aureus on solid surfaces
title_full_unstemmed Confocal spectral microscopy, a non-destructive approach to follow contamination and biofilm formation of mCherry Staphylococcus aureus on solid surfaces
title_sort confocal spectral microscopy, a non-destructive approach to follow contamination and biofilm formation of mcherry staphylococcus aureus on solid surfaces
publisher Nature Publishing Group
series Scientific Reports
issn 2045-2322
publishDate 2021-08-01
description Abstract Methods to test the safety of wood material for hygienically sensitive places are indirect, destructive and limited to incomplete microbial recovery via swabbing, brushing and elution-based techniques. Therefore, we chose mCherry Staphylococcus aureus as a model bacterium for solid and porous surface contamination. Confocal spectral laser microscope (CSLM) was employed to characterize and use the autofluorescence of Sessile oak (Quercus petraea), Douglas fir (Pseudotsuga menziesii) and poplar (Populus euramericana alba L.) wood discs cut into transversal (RT) and tangential (LT) planes. The red fluorescent area occupied by bacteria was differentiated from that of wood, which represented the bacterial quantification, survival and bio-distribution on surfaces from one hour to one week after inoculation. More bacteria were present near the surface on LT face wood as compared to RT and they persisted throughout the study period. Furthermore, this innovative methodology identified that S. aureus formed a dense biofilm on melamine but not on oak wood in similar inoculation and growth conditions. Conclusively, the endogenous fluorescence of materials and the model bacterium permitted direct quantification of surface contamination by using CSLM and it is a promising tool for hygienic safety evaluation.
url https://doi.org/10.1038/s41598-021-94939-2
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