Trypan blue exclusion assay by flow cytometry
Dye exclusion tests are used to determine the number of live and dead cells. These assays are based on the principle that intact plasma membranes in live cells exclude specific dyes, whereas dead cells do not. Although widely used, the trypan blue (TB) exclusion assay has limitations. The dye can be...
Main Authors: | B.A. Avelar-Freitas, V.G. Almeida, M.C.X. Pinto, F.A.G. Mourão, A.R. Massensini, O.A. Martins-Filho, E. Rocha-Vieira, G.E.A. Brito-Melo |
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Format: | Article |
Language: | English |
Published: |
Associação Brasileira de Divulgação Científica
2014-04-01
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Series: | Brazilian Journal of Medical and Biological Research |
Subjects: | |
Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400307&lng=en&tlng=en |
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