Dissemination Pattern of Multidrug Resistant Carbapenemase Producing Klebsiella pneumoniae Isolates Using Pulsed-Field Gel Electrophoresis in Southwestern Iran
Zahra Hashemizadeh,1 Zahra Hosseinzadeh,2 Negar Azimzadeh,3 Mohammad Motamedifar1,4 1Department of Bacteriology and Virology, Shiraz University of Medical Sciences, Shiraz, Iran; 2Basic Sciences in Infectious Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; 3Department...
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doaj-ad58d9c2378448efb8b7bd06b1ccf5052020-11-25T01:54:15ZengDove Medical PressInfection and Drug Resistance1178-69732020-03-01Volume 1392192952813Dissemination Pattern of Multidrug Resistant Carbapenemase Producing Klebsiella pneumoniae Isolates Using Pulsed-Field Gel Electrophoresis in Southwestern IranHashemizadeh ZHosseinzadeh ZAzimzadeh NMotamedifar MZahra Hashemizadeh,1 Zahra Hosseinzadeh,2 Negar Azimzadeh,3 Mohammad Motamedifar1,4 1Department of Bacteriology and Virology, Shiraz University of Medical Sciences, Shiraz, Iran; 2Basic Sciences in Infectious Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; 3Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran; 4Shiraz HIV/Aids Research Center, Institute of Health, Shiraz University of Medical Sciences, Shiraz, IranCorrespondence: Mohammad MotamedifarDepartment of Bacteriology and Virology, Shiraz University of Medical Science, Shiraz, IranTel/Fax +98 71 3230 4356Email motamedm@sums.ac.irBackground: Klebsiella pneumoniae is an important cause of healthcare-associated infection. Carbapenemases have increasingly been reported in Enterobacteriaceae, especially in K. pneumoniae.Propose: The objective of this study was to determine antibiotic resistance patterns, and the molecular epidemiology of multidrug resistant K. pneumoniae isolates, obtained from hospitalized patients in Shiraz, Iran.Methods: In this study, 60 K. pneumoniaeisolates were collected from Nemazee and Faghihi referral hospitals. Antibiotic susceptibility testing and MIC were performed by disk diffusion test and Epsilometer (E)-test strips, respectively. Carbapenemase genes were identified by polymerase chain reaction and sequencing. Then, clonal relationships were analyzed, using PFGE.Results: Thirty-three out of 60 K. pneumoniae isolates were resistant to carbapenems. Among the isolates, 86.6% were multidrug resistant (MDR). Polymyxin B (18.3%) and tigecycline (23.3%) were shown to be the most active agents against K. pneumoniae isolates. In our study, the high prevalence of blaNDM (45%) and blaOXA-48 (10%) was detected.Conclusion: The results of this study revealed the widespread carbapenemase gene between different wards in hospitals as a risk factor for treatment options. PFGE analysis showed 11 clusters and 3 singletons based on an 80% similarity level. Also, PFGE analysis showed that there were similar genetic patterns among K. pneumoniae isolates and these patterns were responsible for the distribution of infection in hospitals.Keywords: Klebsiella pneumoniae, pulsed-field gel electrophoresis, PFGE, carbapenemaseshttps://www.dovepress.com/dissemination-pattern-of-multidrug-resistant-carbapenemase-producing-k-peer-reviewed-article-IDRklebsiella pneumoniapulsed field gel electrophoresis (pfge)carbapenemases |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hashemizadeh Z Hosseinzadeh Z Azimzadeh N Motamedifar M |
spellingShingle |
Hashemizadeh Z Hosseinzadeh Z Azimzadeh N Motamedifar M Dissemination Pattern of Multidrug Resistant Carbapenemase Producing Klebsiella pneumoniae Isolates Using Pulsed-Field Gel Electrophoresis in Southwestern Iran Infection and Drug Resistance klebsiella pneumonia pulsed field gel electrophoresis (pfge) carbapenemases |
author_facet |
Hashemizadeh Z Hosseinzadeh Z Azimzadeh N Motamedifar M |
author_sort |
Hashemizadeh Z |
title |
Dissemination Pattern of Multidrug Resistant Carbapenemase Producing Klebsiella pneumoniae Isolates Using Pulsed-Field Gel Electrophoresis in Southwestern Iran |
title_short |
Dissemination Pattern of Multidrug Resistant Carbapenemase Producing Klebsiella pneumoniae Isolates Using Pulsed-Field Gel Electrophoresis in Southwestern Iran |
title_full |
Dissemination Pattern of Multidrug Resistant Carbapenemase Producing Klebsiella pneumoniae Isolates Using Pulsed-Field Gel Electrophoresis in Southwestern Iran |
title_fullStr |
Dissemination Pattern of Multidrug Resistant Carbapenemase Producing Klebsiella pneumoniae Isolates Using Pulsed-Field Gel Electrophoresis in Southwestern Iran |
title_full_unstemmed |
Dissemination Pattern of Multidrug Resistant Carbapenemase Producing Klebsiella pneumoniae Isolates Using Pulsed-Field Gel Electrophoresis in Southwestern Iran |
title_sort |
dissemination pattern of multidrug resistant carbapenemase producing klebsiella pneumoniae isolates using pulsed-field gel electrophoresis in southwestern iran |
publisher |
Dove Medical Press |
series |
Infection and Drug Resistance |
issn |
1178-6973 |
publishDate |
2020-03-01 |
description |
Zahra Hashemizadeh,1 Zahra Hosseinzadeh,2 Negar Azimzadeh,3 Mohammad Motamedifar1,4 1Department of Bacteriology and Virology, Shiraz University of Medical Sciences, Shiraz, Iran; 2Basic Sciences in Infectious Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; 3Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran; 4Shiraz HIV/Aids Research Center, Institute of Health, Shiraz University of Medical Sciences, Shiraz, IranCorrespondence: Mohammad MotamedifarDepartment of Bacteriology and Virology, Shiraz University of Medical Science, Shiraz, IranTel/Fax +98 71 3230 4356Email motamedm@sums.ac.irBackground: Klebsiella pneumoniae is an important cause of healthcare-associated infection. Carbapenemases have increasingly been reported in Enterobacteriaceae, especially in K. pneumoniae.Propose: The objective of this study was to determine antibiotic resistance patterns, and the molecular epidemiology of multidrug resistant K. pneumoniae isolates, obtained from hospitalized patients in Shiraz, Iran.Methods: In this study, 60 K. pneumoniaeisolates were collected from Nemazee and Faghihi referral hospitals. Antibiotic susceptibility testing and MIC were performed by disk diffusion test and Epsilometer (E)-test strips, respectively. Carbapenemase genes were identified by polymerase chain reaction and sequencing. Then, clonal relationships were analyzed, using PFGE.Results: Thirty-three out of 60 K. pneumoniae isolates were resistant to carbapenems. Among the isolates, 86.6% were multidrug resistant (MDR). Polymyxin B (18.3%) and tigecycline (23.3%) were shown to be the most active agents against K. pneumoniae isolates. In our study, the high prevalence of blaNDM (45%) and blaOXA-48 (10%) was detected.Conclusion: The results of this study revealed the widespread carbapenemase gene between different wards in hospitals as a risk factor for treatment options. PFGE analysis showed 11 clusters and 3 singletons based on an 80% similarity level. Also, PFGE analysis showed that there were similar genetic patterns among K. pneumoniae isolates and these patterns were responsible for the distribution of infection in hospitals.Keywords: Klebsiella pneumoniae, pulsed-field gel electrophoresis, PFGE, carbapenemases |
topic |
klebsiella pneumonia pulsed field gel electrophoresis (pfge) carbapenemases |
url |
https://www.dovepress.com/dissemination-pattern-of-multidrug-resistant-carbapenemase-producing-k-peer-reviewed-article-IDR |
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