Oxidative Damage to the Salivary Glands of Rats with Streptozotocin-Induced Diabetes-Temporal Study: Oxidative Stress and Diabetic Salivary Glands
Objective. This study evaluated oxidative damage caused to the salivary glands in streptozotocin-induced diabetes (DM). Materials and Methods. Rats were divided into 4 groups: groups 1 and 2, control rats, and groups 3 and 4, DM rats. 8-Hydroxy-2′-deoxyguanosine (8-OHdG), protein carbonyl (PC), 4-hy...
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Series: | Journal of Diabetes Research |
Online Access: | http://dx.doi.org/10.1155/2016/4583742 |
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doaj-ad62a0b15f6142ed9438f59858fcfbc72020-11-25T01:05:12ZengHindawi LimitedJournal of Diabetes Research2314-67452314-67532016-01-01201610.1155/2016/45837424583742Oxidative Damage to the Salivary Glands of Rats with Streptozotocin-Induced Diabetes-Temporal Study: Oxidative Stress and Diabetic Salivary GlandsM. Knaś0M. Maciejczyk1I. Daniszewska2A. Klimiuk3J. Matczuk4U. Kołodziej5D. Waszkiel6J. R. Ładny7M. Żendzian-Piotrowska8A. Zalewska9Department of Health Care, Higher Vocational School, Noniewicza 10 Street, 16-400 Suwalki, PolandStudents’ Scientific Group “Stomatological Biochemistry”, Department of Conservative Dentistry, Medical University of Bialystok, Sklodowskiej M.C. 24a Street, 15-274 Bialystok, PolandSpecialist Dental Practice, I. Daniszewska, Żeromskiego 5 Street, 15-225 Bialystok, PolandDepartment of Conservative Dentistry, Medical University Bialystok, Sklodowskiej M.C. 24a Street, 15-274 Bialystok, PolandCounty Veterinary Inspection, Zwycięstwa 26B Street, 15-959 Bialystok, PolandDepartment of Conservative Dentistry, Medical University Bialystok, Sklodowskiej M.C. 24a Street, 15-274 Bialystok, PolandDepartment of Conservative Dentistry, Medical University Bialystok, Sklodowskiej M.C. 24a Street, 15-274 Bialystok, PolandDepartment of Emergency Medicine and Disaster, Medical University of Bialystok, Szpitalna 37 Street, 15-295 Bialystok, PolandDepartment of Hygiene, Epidemiology and Ergonomics, Medical University of Bialystok, Mickiewicza 2c Street, 15-222 Bialystok, PolandDepartment of Conservative Dentistry, Medical University Bialystok, Sklodowskiej M.C. 24a Street, 15-274 Bialystok, PolandObjective. This study evaluated oxidative damage caused to the salivary glands in streptozotocin-induced diabetes (DM). Materials and Methods. Rats were divided into 4 groups: groups 1 and 2, control rats, and groups 3 and 4, DM rats. 8-Hydroxy-2′-deoxyguanosine (8-OHdG), protein carbonyl (PC), 4-hydroxynonenal protein adduct (4-HNE), oxidized and/or MDA-modified LDL-cholesterol (oxy-LDL/MDA), 8-isoprostanes (8-isoP), and oxidative stress index (OSI) were measured at 7 (groups 1 and 3) and 14 (groups 2 and 4) days of experiment. Results. The unstimulated salivary flow in DM rats was reduced in the 2nd week, while the stimulated flow was decreased throughout the duration of the experiment versus control. OSI was elevated in both diabetic glands in the 1st and 2nd week, whereas 8-isoP and 8-OHdG were higher only in the parotid gland in the second week. PC and 4-HNE were increased in the 1st and 2nd week, whereas oxy-LDL/MDA was increased in the 2nd week in the diabetic parotid glands. Conclusions. Diabetes induces oxidative damage of the salivary glands, which seems to be caused by processes taking place in the salivary glands, independently of general oxidative stress. The parotid glands are more vulnerable to oxidative damage in these conditions.http://dx.doi.org/10.1155/2016/4583742 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
M. Knaś M. Maciejczyk I. Daniszewska A. Klimiuk J. Matczuk U. Kołodziej D. Waszkiel J. R. Ładny M. Żendzian-Piotrowska A. Zalewska |
spellingShingle |
M. Knaś M. Maciejczyk I. Daniszewska A. Klimiuk J. Matczuk U. Kołodziej D. Waszkiel J. R. Ładny M. Żendzian-Piotrowska A. Zalewska Oxidative Damage to the Salivary Glands of Rats with Streptozotocin-Induced Diabetes-Temporal Study: Oxidative Stress and Diabetic Salivary Glands Journal of Diabetes Research |
author_facet |
M. Knaś M. Maciejczyk I. Daniszewska A. Klimiuk J. Matczuk U. Kołodziej D. Waszkiel J. R. Ładny M. Żendzian-Piotrowska A. Zalewska |
author_sort |
M. Knaś |
title |
Oxidative Damage to the Salivary Glands of Rats with Streptozotocin-Induced Diabetes-Temporal Study: Oxidative Stress and Diabetic Salivary Glands |
title_short |
Oxidative Damage to the Salivary Glands of Rats with Streptozotocin-Induced Diabetes-Temporal Study: Oxidative Stress and Diabetic Salivary Glands |
title_full |
Oxidative Damage to the Salivary Glands of Rats with Streptozotocin-Induced Diabetes-Temporal Study: Oxidative Stress and Diabetic Salivary Glands |
title_fullStr |
Oxidative Damage to the Salivary Glands of Rats with Streptozotocin-Induced Diabetes-Temporal Study: Oxidative Stress and Diabetic Salivary Glands |
title_full_unstemmed |
Oxidative Damage to the Salivary Glands of Rats with Streptozotocin-Induced Diabetes-Temporal Study: Oxidative Stress and Diabetic Salivary Glands |
title_sort |
oxidative damage to the salivary glands of rats with streptozotocin-induced diabetes-temporal study: oxidative stress and diabetic salivary glands |
publisher |
Hindawi Limited |
series |
Journal of Diabetes Research |
issn |
2314-6745 2314-6753 |
publishDate |
2016-01-01 |
description |
Objective. This study evaluated oxidative damage caused to the salivary glands in streptozotocin-induced diabetes (DM). Materials and Methods. Rats were divided into 4 groups: groups 1 and 2, control rats, and groups 3 and 4, DM rats. 8-Hydroxy-2′-deoxyguanosine (8-OHdG), protein carbonyl (PC), 4-hydroxynonenal protein adduct (4-HNE), oxidized and/or MDA-modified LDL-cholesterol (oxy-LDL/MDA), 8-isoprostanes (8-isoP), and oxidative stress index (OSI) were measured at 7 (groups 1 and 3) and 14 (groups 2 and 4) days of experiment. Results. The unstimulated salivary flow in DM rats was reduced in the 2nd week, while the stimulated flow was decreased throughout the duration of the experiment versus control. OSI was elevated in both diabetic glands in the 1st and 2nd week, whereas 8-isoP and 8-OHdG were higher only in the parotid gland in the second week. PC and 4-HNE were increased in the 1st and 2nd week, whereas oxy-LDL/MDA was increased in the 2nd week in the diabetic parotid glands. Conclusions. Diabetes induces oxidative damage of the salivary glands, which seems to be caused by processes taking place in the salivary glands, independently of general oxidative stress. The parotid glands are more vulnerable to oxidative damage in these conditions. |
url |
http://dx.doi.org/10.1155/2016/4583742 |
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