Evaluation of the antimicrobial activity of various concentrations of Tulsi <i>(Ocimum sanctum)</i> extract against <i>Streptococcus mutans</i>: An <i>in vitro</i> study
<b>Aim:</b> To determine if Tulsi <i>(Ocimum sanctum)</i> extract has an antimicrobial activity against <i>Streptococcus mutans</i> and to determine which concentration of Tulsi <i>(Ocimum sanctum)</i> extract among the 15 concentrations investigated h...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Wolters Kluwer Medknow Publications
2010-01-01
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Series: | Indian Journal of Dental Research |
Subjects: | |
Online Access: | http://www.ijdr.in/article.asp?issn=0970-9290;year=2010;volume=21;issue=3;spage=357;epage=359;aulast=Agarwal |
Summary: | <b>Aim:</b> To determine if Tulsi <i>(Ocimum sanctum)</i> extract has an antimicrobial activity against <i>Streptococcus mutans</i> and to determine which concentration of Tulsi <i>(Ocimum sanctum)</i> extract among the 15 concentrations investigated has the maximum antimicrobial activity. <b>Setting and Design:</b> Experimental design, <i>in vitro</i> study, Lab setting. <b>Materials and Methods:</b> Ethanolic extract of Tulsi was prepared by the cold extraction method. The extract was then diluted with an inert solvent, dimethyl formamide, to obtain 15 different concentrations (0.5%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, 6%, 7% 8%, 9%, 10%) of the extract. 0.2% chlorhexidine was used as a positive control and dimethyl formamide was used as a negative control. The extract, along with the controls, was then subjected to microbiological investigation to determine which concentration among the 15 different concentrations of the extract gave a wider inhibition zone against <i>Streptococcus mutans</i>. The zones of inhibition were measured in millimeters using a vernier caliper. <b>Results:</b> At the 4% concentration of Tulsi extract, a zone of inhibition of 22 mm was obtained. This was the widest zone of inhibition observed among all the 15 different concentrations of Tulsi that were investigated. <b>Conclusion:</b> Tulsi extract demonstrated an antimicrobial property against <i>Streptococcus mutans</i>. |
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ISSN: | 0970-9290 1998-3603 |