Evaluation of organ glucose metabolism by 18F-FDG accumulation with insulin loading in aged mice compared with young normal mice
Abstract It is important to determine the functional changes of organs that occur as a result of aging, the understanding of which may lead to the maintenance of a healthy life. Glucose metabolism in healthy bodies is one of the potential markers used to evaluate the changes of organ function. Thus,...
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doaj-ad808f0989604a349872e588c551ada82021-04-04T11:32:43ZengNature Publishing GroupScientific Reports2045-23222021-04-0111111010.1038/s41598-021-86825-8Evaluation of organ glucose metabolism by 18F-FDG accumulation with insulin loading in aged mice compared with young normal miceJingmin Zhao0Chengbo Tan1Ryota Imai2Naoyuki Ukon3Saki Shimoyama4Yuko Maejima5Yuji Omiya6Kazuhiro Takahashi7Hiroshi Ito8Guangxian Nan9Songji Zhao10Kenju Shimomura11Department of Neurology, China-Japan Union Hospital of Jilin UniversityDepartment of Neurosurgery, Shanghai East Hospital, Tongji University School of MedicineDepartment of Bioregulation and Pharmacological Medicine, Fukushima Medical UniversityAdvanced Clinical Research Center, Fukushima Global Medical Science Center, Fukushima Medical UniversityAdvanced Clinical Research Center, Fukushima Global Medical Science Center, Fukushima Medical UniversityDepartment of Bioregulation and Pharmacological Medicine, Fukushima Medical UniversityTsumura Kampo Research Laboratories, Kampo Research & Development Division, Tsumura & Co.Advanced Clinical Research Center, Fukushima Global Medical Science Center, Fukushima Medical UniversityDepartment of Radiology and Nuclear Medicine, Fukushima Medical UniversityDepartment of Neurology, China-Japan Union Hospital of Jilin UniversityAdvanced Clinical Research Center, Fukushima Global Medical Science Center, Fukushima Medical UniversityDepartment of Bioregulation and Pharmacological Medicine, Fukushima Medical UniversityAbstract It is important to determine the functional changes of organs that occur as a result of aging, the understanding of which may lead to the maintenance of a healthy life. Glucose metabolism in healthy bodies is one of the potential markers used to evaluate the changes of organ function. Thus, information about normal organ glucose metabolism may help to understand the functional changes of organs. [18F]-Fluoro-2-deoxy-2-d-glucose (18F-FDG), a glucose analog, has been used to measure glucose metabolism in various fields, such as basic medical research and drug discovery. However, glucose metabolism changes in aged animals have not yet been fully clarified. The aim of this study is to evaluate changes in glucose metabolism in organs and brain regions by measuring 18F-FDG accumulation and 18F-FDG autoradiography with insulin loading in aged and young wild-type mice. In the untreated groups, the levels of 18F-FDG accumulation in the blood, plasma, muscle, lungs, spleen, pancreas, testes, stomach, small intestine, kidneys, liver, brain, and brain regions, namely, the cortex, striatum, thalamus, and hippocampus, were all significantly higher in the aged mice. The treated group showed lower 18F-FDG accumulation levels in the pancreas and kidneys, as well as in the cortex, striatum, thalamus, and hippocampus in the aged mice than the untreated groups, whereas higher 18F-FDG accumulation levels were observed in those in the young mice. These results demonstrate that insulin loading decreases effect on 18F-FDG accumulation levels in some organs of the aged mice. Therefore, aging can increase insulin resistance and lead to systemic glucose metabolism dysfunction.https://doi.org/10.1038/s41598-021-86825-8 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jingmin Zhao Chengbo Tan Ryota Imai Naoyuki Ukon Saki Shimoyama Yuko Maejima Yuji Omiya Kazuhiro Takahashi Hiroshi Ito Guangxian Nan Songji Zhao Kenju Shimomura |
spellingShingle |
Jingmin Zhao Chengbo Tan Ryota Imai Naoyuki Ukon Saki Shimoyama Yuko Maejima Yuji Omiya Kazuhiro Takahashi Hiroshi Ito Guangxian Nan Songji Zhao Kenju Shimomura Evaluation of organ glucose metabolism by 18F-FDG accumulation with insulin loading in aged mice compared with young normal mice Scientific Reports |
author_facet |
Jingmin Zhao Chengbo Tan Ryota Imai Naoyuki Ukon Saki Shimoyama Yuko Maejima Yuji Omiya Kazuhiro Takahashi Hiroshi Ito Guangxian Nan Songji Zhao Kenju Shimomura |
author_sort |
Jingmin Zhao |
title |
Evaluation of organ glucose metabolism by 18F-FDG accumulation with insulin loading in aged mice compared with young normal mice |
title_short |
Evaluation of organ glucose metabolism by 18F-FDG accumulation with insulin loading in aged mice compared with young normal mice |
title_full |
Evaluation of organ glucose metabolism by 18F-FDG accumulation with insulin loading in aged mice compared with young normal mice |
title_fullStr |
Evaluation of organ glucose metabolism by 18F-FDG accumulation with insulin loading in aged mice compared with young normal mice |
title_full_unstemmed |
Evaluation of organ glucose metabolism by 18F-FDG accumulation with insulin loading in aged mice compared with young normal mice |
title_sort |
evaluation of organ glucose metabolism by 18f-fdg accumulation with insulin loading in aged mice compared with young normal mice |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2021-04-01 |
description |
Abstract It is important to determine the functional changes of organs that occur as a result of aging, the understanding of which may lead to the maintenance of a healthy life. Glucose metabolism in healthy bodies is one of the potential markers used to evaluate the changes of organ function. Thus, information about normal organ glucose metabolism may help to understand the functional changes of organs. [18F]-Fluoro-2-deoxy-2-d-glucose (18F-FDG), a glucose analog, has been used to measure glucose metabolism in various fields, such as basic medical research and drug discovery. However, glucose metabolism changes in aged animals have not yet been fully clarified. The aim of this study is to evaluate changes in glucose metabolism in organs and brain regions by measuring 18F-FDG accumulation and 18F-FDG autoradiography with insulin loading in aged and young wild-type mice. In the untreated groups, the levels of 18F-FDG accumulation in the blood, plasma, muscle, lungs, spleen, pancreas, testes, stomach, small intestine, kidneys, liver, brain, and brain regions, namely, the cortex, striatum, thalamus, and hippocampus, were all significantly higher in the aged mice. The treated group showed lower 18F-FDG accumulation levels in the pancreas and kidneys, as well as in the cortex, striatum, thalamus, and hippocampus in the aged mice than the untreated groups, whereas higher 18F-FDG accumulation levels were observed in those in the young mice. These results demonstrate that insulin loading decreases effect on 18F-FDG accumulation levels in some organs of the aged mice. Therefore, aging can increase insulin resistance and lead to systemic glucose metabolism dysfunction. |
url |
https://doi.org/10.1038/s41598-021-86825-8 |
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