Combined Extracts of Herba Epimedii and Fructus Ligustri Lucidi Rebalance Bone Remodeling in Ovariectomized Rats
This study aimed to investigate the osteoprotective effect and the possible molecular mechanisms of the combined extracts of Herba Epimedii and Fructus Ligustri Lucidi on postmenopausal osteoporosis (PMOP). Forty-eight female SD rats were sham-operated (Sham, n = 8) or ovariectomized (OVX, n = 40)....
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2019-01-01
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Series: | Evidence-Based Complementary and Alternative Medicine |
Online Access: | http://dx.doi.org/10.1155/2019/1596951 |
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doaj-ad82be621f9548169be27d80f7321c9b2020-11-25T00:28:38ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882019-01-01201910.1155/2019/15969511596951Combined Extracts of Herba Epimedii and Fructus Ligustri Lucidi Rebalance Bone Remodeling in Ovariectomized RatsYuheng Chen0Xiaoxi Li1Xiufeng Tang2Yingying Gao3Ping Yu4Liping Xu5Renhui Liu6School of Traditional Chinese Medicine, Capital Medical University and Beijing Key Lab of TCM Collateral Disease Theory Research, No. 10 Xitoutiao, Youanmenwai, Fengtai District, Beijing 100069, ChinaSchool of Traditional Chinese Medicine, Capital Medical University and Beijing Key Lab of TCM Collateral Disease Theory Research, No. 10 Xitoutiao, Youanmenwai, Fengtai District, Beijing 100069, ChinaSchool of Traditional Chinese Medicine, Capital Medical University and Beijing Key Lab of TCM Collateral Disease Theory Research, No. 10 Xitoutiao, Youanmenwai, Fengtai District, Beijing 100069, ChinaSchool of Traditional Chinese Medicine, Capital Medical University and Beijing Key Lab of TCM Collateral Disease Theory Research, No. 10 Xitoutiao, Youanmenwai, Fengtai District, Beijing 100069, ChinaSchool of Traditional Chinese Medicine, Capital Medical University and Beijing Key Lab of TCM Collateral Disease Theory Research, No. 10 Xitoutiao, Youanmenwai, Fengtai District, Beijing 100069, ChinaSchool of Traditional Chinese Medicine, Capital Medical University and Beijing Key Lab of TCM Collateral Disease Theory Research, No. 10 Xitoutiao, Youanmenwai, Fengtai District, Beijing 100069, ChinaSchool of Traditional Chinese Medicine, Capital Medical University and Beijing Key Lab of TCM Collateral Disease Theory Research, No. 10 Xitoutiao, Youanmenwai, Fengtai District, Beijing 100069, ChinaThis study aimed to investigate the osteoprotective effect and the possible molecular mechanisms of the combined extracts of Herba Epimedii and Fructus Ligustri Lucidi on postmenopausal osteoporosis (PMOP). Forty-eight female SD rats were sham-operated (Sham, n = 8) or ovariectomized (OVX, n = 40). Then after a week, OVX rats were divided randomly into five groups (n = 8 in each group): OVX, extracts of Herba Epimedii (HE, 0.35 g/kg), extracts of Fructus Ligustri Lucidi (FLL, 0.35 g/kg), combined extracts of HE and FLL (HE & FLL, 0.20 g/kg HE plus 0.15 g/kg FLL), and Raloxifene hydrochloride (RH, 6.25 mg/kg) groups. All groups were administered once daily for 12 weeks. Indicators related to bone remodeling were detected, including estradiol (E2), bone mineral density (BMD), maximal load, ultimate deflection, micro-CT properties, tartrate-resistant acid phosphatase (TRACP) and alkaline phosphatase (ALP) levels in serum and bone, and the protein and mRNA expression of bone turnover markers (RANKL, M-CSF, Wnt5a, Atp6v0d2, OPG, IGF-1, TGF-β1, and Bmp-2). Results showed that the combined extracts could increase serum E2 levels and BMD, enhance bone strength, reserve bone microstructure degeneration, promote bone formation, and inhibit bone resorption through upregulating the mRNA and protein expression of OPG, IGF-1, TGF-β1, and Bmp-2, while downregulating RANKL, M-CSF, Wnt5a, and Atp6v0d2. These findings demonstrated that the combined extracts of Herba Epimedii and Fructus Ligustri Lucidi with bone protective effects on OVX rats might be an alternative medicine for the treatment of PMOP.http://dx.doi.org/10.1155/2019/1596951 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yuheng Chen Xiaoxi Li Xiufeng Tang Yingying Gao Ping Yu Liping Xu Renhui Liu |
spellingShingle |
Yuheng Chen Xiaoxi Li Xiufeng Tang Yingying Gao Ping Yu Liping Xu Renhui Liu Combined Extracts of Herba Epimedii and Fructus Ligustri Lucidi Rebalance Bone Remodeling in Ovariectomized Rats Evidence-Based Complementary and Alternative Medicine |
author_facet |
Yuheng Chen Xiaoxi Li Xiufeng Tang Yingying Gao Ping Yu Liping Xu Renhui Liu |
author_sort |
Yuheng Chen |
title |
Combined Extracts of Herba Epimedii and Fructus Ligustri Lucidi Rebalance Bone Remodeling in Ovariectomized Rats |
title_short |
Combined Extracts of Herba Epimedii and Fructus Ligustri Lucidi Rebalance Bone Remodeling in Ovariectomized Rats |
title_full |
Combined Extracts of Herba Epimedii and Fructus Ligustri Lucidi Rebalance Bone Remodeling in Ovariectomized Rats |
title_fullStr |
Combined Extracts of Herba Epimedii and Fructus Ligustri Lucidi Rebalance Bone Remodeling in Ovariectomized Rats |
title_full_unstemmed |
Combined Extracts of Herba Epimedii and Fructus Ligustri Lucidi Rebalance Bone Remodeling in Ovariectomized Rats |
title_sort |
combined extracts of herba epimedii and fructus ligustri lucidi rebalance bone remodeling in ovariectomized rats |
publisher |
Hindawi Limited |
series |
Evidence-Based Complementary and Alternative Medicine |
issn |
1741-427X 1741-4288 |
publishDate |
2019-01-01 |
description |
This study aimed to investigate the osteoprotective effect and the possible molecular mechanisms of the combined extracts of Herba Epimedii and Fructus Ligustri Lucidi on postmenopausal osteoporosis (PMOP). Forty-eight female SD rats were sham-operated (Sham, n = 8) or ovariectomized (OVX, n = 40). Then after a week, OVX rats were divided randomly into five groups (n = 8 in each group): OVX, extracts of Herba Epimedii (HE, 0.35 g/kg), extracts of Fructus Ligustri Lucidi (FLL, 0.35 g/kg), combined extracts of HE and FLL (HE & FLL, 0.20 g/kg HE plus 0.15 g/kg FLL), and Raloxifene hydrochloride (RH, 6.25 mg/kg) groups. All groups were administered once daily for 12 weeks. Indicators related to bone remodeling were detected, including estradiol (E2), bone mineral density (BMD), maximal load, ultimate deflection, micro-CT properties, tartrate-resistant acid phosphatase (TRACP) and alkaline phosphatase (ALP) levels in serum and bone, and the protein and mRNA expression of bone turnover markers (RANKL, M-CSF, Wnt5a, Atp6v0d2, OPG, IGF-1, TGF-β1, and Bmp-2). Results showed that the combined extracts could increase serum E2 levels and BMD, enhance bone strength, reserve bone microstructure degeneration, promote bone formation, and inhibit bone resorption through upregulating the mRNA and protein expression of OPG, IGF-1, TGF-β1, and Bmp-2, while downregulating RANKL, M-CSF, Wnt5a, and Atp6v0d2. These findings demonstrated that the combined extracts of Herba Epimedii and Fructus Ligustri Lucidi with bone protective effects on OVX rats might be an alternative medicine for the treatment of PMOP. |
url |
http://dx.doi.org/10.1155/2019/1596951 |
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