In Vitro Cytoprotective Effects and Antioxidant Capacity of Phenolic Compounds from the Leaves of Swietenia macrophylla
Swietenia macrophylla (mahogany) is a highly valued timber species, whereas the leaves are considered to be waste product. A total of 27 phenolic compounds were identified in aqueous extracts from mahogany leaves by comparing retention times and mass spectra data with those of authentic standards us...
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doaj-ad8cc42acab94b5b9b1bd57c51c445ac2020-11-24T22:24:24ZengMDPI AGMolecules1420-30492015-10-012010187771878810.3390/molecules201018777molecules201018777In Vitro Cytoprotective Effects and Antioxidant Capacity of Phenolic Compounds from the Leaves of Swietenia macrophyllaSônia Pamplona0Paulo Sá1Dielly Lopes2Edmar Costa3Elizabeth Yamada4Consuelo e Silva5Mara Arruda6Jesus Souza7Milton da Silva8Programa de Pós-Graduação em Química, Instituto de Ciências Exatas e Naturais, Campus Universitário do Guamá, Universidade Federal do Pará, Belém-PA 66075-970, BrazilPrograma de Pós-Graduação em Química, Instituto de Ciências Exatas e Naturais, Campus Universitário do Guamá, Universidade Federal do Pará, Belém-PA 66075-970, BrazilLaboratório de Neuropatologia Experimental (LaNEx), Hospital Universitário Barros Barreto, Rua dos Mundurucus, 4487–Guamá. CEP 66073-000. Belém, PA, BrazilLaboratório de Neuropatologia Experimental (LaNEx), Hospital Universitário Barros Barreto, Rua dos Mundurucus, 4487–Guamá. CEP 66073-000. Belém, PA, BrazilLaboratório de Neuropatologia Experimental (LaNEx), Hospital Universitário Barros Barreto, Rua dos Mundurucus, 4487–Guamá. CEP 66073-000. Belém, PA, BrazilPrograma de Pós-Graduação em Química, Instituto de Ciências Exatas e Naturais, Campus Universitário do Guamá, Universidade Federal do Pará, Belém-PA 66075-970, BrazilPrograma de Pós-Graduação em Química, Instituto de Ciências Exatas e Naturais, Campus Universitário do Guamá, Universidade Federal do Pará, Belém-PA 66075-970, BrazilFaculdade de Engenharia de Alimentos, Instituto de Tecnologia, Campus Universitário do Guamá, Universidade Federal do Pará, Belém-PA 66075-970, BrazilPrograma de Pós-Graduação em Química, Instituto de Ciências Exatas e Naturais, Campus Universitário do Guamá, Universidade Federal do Pará, Belém-PA 66075-970, BrazilSwietenia macrophylla (mahogany) is a highly valued timber species, whereas the leaves are considered to be waste product. A total of 27 phenolic compounds were identified in aqueous extracts from mahogany leaves by comparing retention times and mass spectra data with those of authentic standards using LC-ESI-MS/MS. Polyphenols play an important role in plants as defense mechanisms against pests and pathogens and have potent antioxidant properties. In terms of health applications, interest has increased considerably in naturally occurring antioxidant sources, since they can retard the progress of many important neurodegenerative diseases such as Alzheimer’s and Parkinson’s diseases. The antioxidant capacities of two aqueous extracts, M1 (decoction) and M2 (infusion), were measured using TEAC and Folin-Ciocalteau methods. Additionally, M1 was used in order to investigate its potential cytoprotective effects on an in vitro model of neurodegeneration, by using primary cerebellar cultures exposed to methyl mercury (MeHg). Under experimental sub-chronic conditions (72 h), concomitant exposure of the same cultures to MeHg and M1 extract resulted in a statistically significant increase in cell viability in all three concentrations tested (10, 50 and 100 μg/mL), strongly suggesting that due to its high content of antioxidant compounds, the M1 extract provides significant cytoprotection against the MeHg-induced in vitro neurotoxicity.http://www.mdpi.com/1420-3049/20/10/18777Swietenia macrophyllacytoprotectionantioxidant capacitypolyphenolsLC-MS/MS |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sônia Pamplona Paulo Sá Dielly Lopes Edmar Costa Elizabeth Yamada Consuelo e Silva Mara Arruda Jesus Souza Milton da Silva |
spellingShingle |
Sônia Pamplona Paulo Sá Dielly Lopes Edmar Costa Elizabeth Yamada Consuelo e Silva Mara Arruda Jesus Souza Milton da Silva In Vitro Cytoprotective Effects and Antioxidant Capacity of Phenolic Compounds from the Leaves of Swietenia macrophylla Molecules Swietenia macrophylla cytoprotection antioxidant capacity polyphenols LC-MS/MS |
author_facet |
Sônia Pamplona Paulo Sá Dielly Lopes Edmar Costa Elizabeth Yamada Consuelo e Silva Mara Arruda Jesus Souza Milton da Silva |
author_sort |
Sônia Pamplona |
title |
In Vitro Cytoprotective Effects and Antioxidant Capacity of Phenolic Compounds from the Leaves of Swietenia macrophylla |
title_short |
In Vitro Cytoprotective Effects and Antioxidant Capacity of Phenolic Compounds from the Leaves of Swietenia macrophylla |
title_full |
In Vitro Cytoprotective Effects and Antioxidant Capacity of Phenolic Compounds from the Leaves of Swietenia macrophylla |
title_fullStr |
In Vitro Cytoprotective Effects and Antioxidant Capacity of Phenolic Compounds from the Leaves of Swietenia macrophylla |
title_full_unstemmed |
In Vitro Cytoprotective Effects and Antioxidant Capacity of Phenolic Compounds from the Leaves of Swietenia macrophylla |
title_sort |
in vitro cytoprotective effects and antioxidant capacity of phenolic compounds from the leaves of swietenia macrophylla |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2015-10-01 |
description |
Swietenia macrophylla (mahogany) is a highly valued timber species, whereas the leaves are considered to be waste product. A total of 27 phenolic compounds were identified in aqueous extracts from mahogany leaves by comparing retention times and mass spectra data with those of authentic standards using LC-ESI-MS/MS. Polyphenols play an important role in plants as defense mechanisms against pests and pathogens and have potent antioxidant properties. In terms of health applications, interest has increased considerably in naturally occurring antioxidant sources, since they can retard the progress of many important neurodegenerative diseases such as Alzheimer’s and Parkinson’s diseases. The antioxidant capacities of two aqueous extracts, M1 (decoction) and M2 (infusion), were measured using TEAC and Folin-Ciocalteau methods. Additionally, M1 was used in order to investigate its potential cytoprotective effects on an in vitro model of neurodegeneration, by using primary cerebellar cultures exposed to methyl mercury (MeHg). Under experimental sub-chronic conditions (72 h), concomitant exposure of the same cultures to MeHg and M1 extract resulted in a statistically significant increase in cell viability in all three concentrations tested (10, 50 and 100 μg/mL), strongly suggesting that due to its high content of antioxidant compounds, the M1 extract provides significant cytoprotection against the MeHg-induced in vitro neurotoxicity. |
topic |
Swietenia macrophylla cytoprotection antioxidant capacity polyphenols LC-MS/MS |
url |
http://www.mdpi.com/1420-3049/20/10/18777 |
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