Optimization of Endoglucanase Production from a Novel Bacterial Isolate, Arthrobacter sp. HPG166 and Characterization of Its Properties
ABSTRACTIn this study, a potential novel cellulolytic bacteriumArthrobacter sp. HPG166 was isolated from the hindgut of root-feeding larvaeHolotrichia parallela. Optimization of fermentation factors for endoglucanase production byArthrobacter sp. HPG166 was carried out via response surface methodolo...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Instituto de Tecnologia do Paraná (Tecpar)
2015-10-01
|
Series: | Brazilian Archives of Biology and Technology |
Subjects: | |
Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500692&lng=en&tlng=en |
id |
doaj-ad928506d26a423cba551b0b614ba424 |
---|---|
record_format |
Article |
spelling |
doaj-ad928506d26a423cba551b0b614ba4242020-11-24T23:38:11ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1678-43242015-10-0158569270110.1590/S1516-89132015050256S1516-89132015000500692Optimization of Endoglucanase Production from a Novel Bacterial Isolate, Arthrobacter sp. HPG166 and Characterization of Its PropertiesShengwei HuangGuanjun DengYing YangZhengyan WuLifang WuABSTRACTIn this study, a potential novel cellulolytic bacteriumArthrobacter sp. HPG166 was isolated from the hindgut of root-feeding larvaeHolotrichia parallela. Optimization of fermentation factors for endoglucanase production byArthrobacter sp. HPG166 was carried out via response surface methodology. Sodium carboxymethylcellulose 1.19% (w/v) and beef extract 0.35% (w/v) were the ideal combination of carbon and nitrogen sources for enzyme production; the optimum temperature and pH for cellulase production were 34°C and pH 8.0 respectively. Under the optimized fermentation conditions, the maximum endoglucanase activity of 1.411 U mL-1 was obtained. The crude endoglucanase was thermotolerant as it retained 50.31% of its activity after incubation at 70°C for an hour. Metal profile of the enzyme indicated that Mg2+ and Na+ were strong stimulators while Mn2+ and Co+ drastically inhibited its activity. Due to its particular characteristics, this enzyme could have potential for industrial applications.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500692&lng=en&tlng=enScarab larvaeCellulolytic bacteriaResponse surface methodologyThermotolerant cellulasesEffects of Metal ions |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shengwei Huang Guanjun Deng Ying Yang Zhengyan Wu Lifang Wu |
spellingShingle |
Shengwei Huang Guanjun Deng Ying Yang Zhengyan Wu Lifang Wu Optimization of Endoglucanase Production from a Novel Bacterial Isolate, Arthrobacter sp. HPG166 and Characterization of Its Properties Brazilian Archives of Biology and Technology Scarab larvae Cellulolytic bacteria Response surface methodology Thermotolerant cellulases Effects of Metal ions |
author_facet |
Shengwei Huang Guanjun Deng Ying Yang Zhengyan Wu Lifang Wu |
author_sort |
Shengwei Huang |
title |
Optimization of Endoglucanase Production from a Novel Bacterial Isolate, Arthrobacter sp. HPG166 and Characterization of Its Properties |
title_short |
Optimization of Endoglucanase Production from a Novel Bacterial Isolate, Arthrobacter sp. HPG166 and Characterization of Its Properties |
title_full |
Optimization of Endoglucanase Production from a Novel Bacterial Isolate, Arthrobacter sp. HPG166 and Characterization of Its Properties |
title_fullStr |
Optimization of Endoglucanase Production from a Novel Bacterial Isolate, Arthrobacter sp. HPG166 and Characterization of Its Properties |
title_full_unstemmed |
Optimization of Endoglucanase Production from a Novel Bacterial Isolate, Arthrobacter sp. HPG166 and Characterization of Its Properties |
title_sort |
optimization of endoglucanase production from a novel bacterial isolate, arthrobacter sp. hpg166 and characterization of its properties |
publisher |
Instituto de Tecnologia do Paraná (Tecpar) |
series |
Brazilian Archives of Biology and Technology |
issn |
1678-4324 |
publishDate |
2015-10-01 |
description |
ABSTRACTIn this study, a potential novel cellulolytic bacteriumArthrobacter sp. HPG166 was isolated from the hindgut of root-feeding larvaeHolotrichia parallela. Optimization of fermentation factors for endoglucanase production byArthrobacter sp. HPG166 was carried out via response surface methodology. Sodium carboxymethylcellulose 1.19% (w/v) and beef extract 0.35% (w/v) were the ideal combination of carbon and nitrogen sources for enzyme production; the optimum temperature and pH for cellulase production were 34°C and pH 8.0 respectively. Under the optimized fermentation conditions, the maximum endoglucanase activity of 1.411 U mL-1 was obtained. The crude endoglucanase was thermotolerant as it retained 50.31% of its activity after incubation at 70°C for an hour. Metal profile of the enzyme indicated that Mg2+ and Na+ were strong stimulators while Mn2+ and Co+ drastically inhibited its activity. Due to its particular characteristics, this enzyme could have potential for industrial applications. |
topic |
Scarab larvae Cellulolytic bacteria Response surface methodology Thermotolerant cellulases Effects of Metal ions |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500692&lng=en&tlng=en |
work_keys_str_mv |
AT shengweihuang optimizationofendoglucanaseproductionfromanovelbacterialisolatearthrobactersphpg166andcharacterizationofitsproperties AT guanjundeng optimizationofendoglucanaseproductionfromanovelbacterialisolatearthrobactersphpg166andcharacterizationofitsproperties AT yingyang optimizationofendoglucanaseproductionfromanovelbacterialisolatearthrobactersphpg166andcharacterizationofitsproperties AT zhengyanwu optimizationofendoglucanaseproductionfromanovelbacterialisolatearthrobactersphpg166andcharacterizationofitsproperties AT lifangwu optimizationofendoglucanaseproductionfromanovelbacterialisolatearthrobactersphpg166andcharacterizationofitsproperties |
_version_ |
1725517681996595200 |