The packing density of a supramolecular membrane protein cluster is controlled by cytoplasmic interactions
Molecule clustering is an important mechanism underlying cellular self-organization. In the cell membrane, a variety of fundamentally different mechanisms drive membrane protein clustering into nanometre-sized assemblies. To date, it is unknown whether this clustering process can be dissected into s...
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doaj-ad9ba51053bf43518e6bbe6bd92602512021-05-05T13:37:25ZengeLife Sciences Publications LtdeLife2050-084X2017-07-01610.7554/eLife.20705The packing density of a supramolecular membrane protein cluster is controlled by cytoplasmic interactionsElisa Merklinger0https://orcid.org/0000-0002-7257-1895Jan-Gero Schloetel1Pascal Weber2https://orcid.org/0000-0002-6369-2708Helena Batoulis3Sarah Holz4Nora Karnowski5Jérôme Finke6Thorsten Lang7https://orcid.org/0000-0002-9128-0137Membrane Biochemistry, Life and Medical Sciences (LIMES) Institute, University of Bonn, Bonn, GermanyMembrane Biochemistry, Life and Medical Sciences (LIMES) Institute, University of Bonn, Bonn, GermanyMembrane Biochemistry, Life and Medical Sciences (LIMES) Institute, University of Bonn, Bonn, GermanyMembrane Biochemistry, Life and Medical Sciences (LIMES) Institute, University of Bonn, Bonn, GermanyMembrane Biochemistry, Life and Medical Sciences (LIMES) Institute, University of Bonn, Bonn, GermanyChemical Biology, Life and Medical Sciences (LIMES) Institute, University of Bonn, Bonn, GermanyMembrane Biochemistry, Life and Medical Sciences (LIMES) Institute, University of Bonn, Bonn, GermanyMembrane Biochemistry, Life and Medical Sciences (LIMES) Institute, University of Bonn, Bonn, GermanyMolecule clustering is an important mechanism underlying cellular self-organization. In the cell membrane, a variety of fundamentally different mechanisms drive membrane protein clustering into nanometre-sized assemblies. To date, it is unknown whether this clustering process can be dissected into steps differentially regulated by independent mechanisms. Using clustered syntaxin molecules as an example, we study the influence of a cytoplasmic protein domain on the clustering behaviour. Analysing protein mobility, cluster size and accessibility to myc-epitopes we show that forces acting on the transmembrane segment produce loose clusters, while cytoplasmic protein interactions mediate a tightly packed state. We conclude that the data identify a hierarchy in membrane protein clustering likely being a paradigm for many cellular self-organization processes.https://elifesciences.org/articles/20705self-organizationclusteringplasma membraneSNAREsmicrodomainsnano-assemblies |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Elisa Merklinger Jan-Gero Schloetel Pascal Weber Helena Batoulis Sarah Holz Nora Karnowski Jérôme Finke Thorsten Lang |
spellingShingle |
Elisa Merklinger Jan-Gero Schloetel Pascal Weber Helena Batoulis Sarah Holz Nora Karnowski Jérôme Finke Thorsten Lang The packing density of a supramolecular membrane protein cluster is controlled by cytoplasmic interactions eLife self-organization clustering plasma membrane SNAREs microdomains nano-assemblies |
author_facet |
Elisa Merklinger Jan-Gero Schloetel Pascal Weber Helena Batoulis Sarah Holz Nora Karnowski Jérôme Finke Thorsten Lang |
author_sort |
Elisa Merklinger |
title |
The packing density of a supramolecular membrane protein cluster is controlled by cytoplasmic interactions |
title_short |
The packing density of a supramolecular membrane protein cluster is controlled by cytoplasmic interactions |
title_full |
The packing density of a supramolecular membrane protein cluster is controlled by cytoplasmic interactions |
title_fullStr |
The packing density of a supramolecular membrane protein cluster is controlled by cytoplasmic interactions |
title_full_unstemmed |
The packing density of a supramolecular membrane protein cluster is controlled by cytoplasmic interactions |
title_sort |
packing density of a supramolecular membrane protein cluster is controlled by cytoplasmic interactions |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2017-07-01 |
description |
Molecule clustering is an important mechanism underlying cellular self-organization. In the cell membrane, a variety of fundamentally different mechanisms drive membrane protein clustering into nanometre-sized assemblies. To date, it is unknown whether this clustering process can be dissected into steps differentially regulated by independent mechanisms. Using clustered syntaxin molecules as an example, we study the influence of a cytoplasmic protein domain on the clustering behaviour. Analysing protein mobility, cluster size and accessibility to myc-epitopes we show that forces acting on the transmembrane segment produce loose clusters, while cytoplasmic protein interactions mediate a tightly packed state. We conclude that the data identify a hierarchy in membrane protein clustering likely being a paradigm for many cellular self-organization processes. |
topic |
self-organization clustering plasma membrane SNAREs microdomains nano-assemblies |
url |
https://elifesciences.org/articles/20705 |
work_keys_str_mv |
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