Rhizobacterial Strain Bacillus megaterium BOFC15 Induces Cellular Polyamine Changes that Improve Plant Growth and Drought Resistance
Plant-growth-promoting rhizobacteria can improve plant growth, development, and stress adaptation. However, the underlying mechanisms are still largely unclear. We investigated the effects of Bacillus megaterium BOFC15 on Arabidopsis plants. BOFC15 produced and secreted spermidine (Spd), a type of p...
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doaj-adab72ba61124d9aa30a7bcf4f9b961e2020-11-24T21:32:58ZengMDPI AGInternational Journal of Molecular Sciences1422-00672016-06-0117697610.3390/ijms17060976ijms17060976Rhizobacterial Strain Bacillus megaterium BOFC15 Induces Cellular Polyamine Changes that Improve Plant Growth and Drought ResistanceCheng Zhou0Zhongyou Ma1Lin Zhu2Xin Xiao3Yue Xie4Jian Zhu5Jianfei Wang6School of Life Science and Technology, Tongji University, Shanghai 200092, ChinaKey Laboratory of Bio-Organic Fertilizer Creation, Ministry of Agriculture, Anhui Science and Technology University, Bengbu 233100, ChinaSchool of Life Science and Technology, Tongji University, Shanghai 200092, ChinaKey Laboratory of Bio-Organic Fertilizer Creation, Ministry of Agriculture, Anhui Science and Technology University, Bengbu 233100, ChinaKey Laboratory of Bio-Organic Fertilizer Creation, Ministry of Agriculture, Anhui Science and Technology University, Bengbu 233100, ChinaSchool of Life Science and Technology, Tongji University, Shanghai 200092, ChinaKey Laboratory of Bio-Organic Fertilizer Creation, Ministry of Agriculture, Anhui Science and Technology University, Bengbu 233100, ChinaPlant-growth-promoting rhizobacteria can improve plant growth, development, and stress adaptation. However, the underlying mechanisms are still largely unclear. We investigated the effects of Bacillus megaterium BOFC15 on Arabidopsis plants. BOFC15 produced and secreted spermidine (Spd), a type of polyamine (PA) that plays an important role in plant growth. Moreover, BOFC15 induced changes in the cellular PAs of plants that promoted an increase of free Spd and spermine levels. However, these effects were remarkably abolished by the addition of dicyclohexylamine (DCHA), a Spd biosynthetic inhibitor. Additionally, the inoculation with BOFC15 remarkably increased plant biomass, improved root system architecture, and augmented photosynthetic capacity. Inoculated plants also displayed stronger ability to tolerate drought stress than non-inoculated (control) plants. Abscisic acid (ABA) content was notably higher in the inoculated plants than in the control plants under drought stress and polyethylene glycol (PEG)-induced stress conditions. However, the BOFC15-induced ABA synthesis was markedly inhibited by DCHA. Thus, microbial Spd participated in the modulation of the ABA levels. The Spd-producing BOFC15 improved plant drought tolerance, which was associated with altered cellular ABA levels and activated adaptive responses.http://www.mdpi.com/1422-0067/17/6/976Bacillus megateriumpolyaminesdrought toleranceabscisic acidArabidopsis thaliana |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Cheng Zhou Zhongyou Ma Lin Zhu Xin Xiao Yue Xie Jian Zhu Jianfei Wang |
spellingShingle |
Cheng Zhou Zhongyou Ma Lin Zhu Xin Xiao Yue Xie Jian Zhu Jianfei Wang Rhizobacterial Strain Bacillus megaterium BOFC15 Induces Cellular Polyamine Changes that Improve Plant Growth and Drought Resistance International Journal of Molecular Sciences Bacillus megaterium polyamines drought tolerance abscisic acid Arabidopsis thaliana |
author_facet |
Cheng Zhou Zhongyou Ma Lin Zhu Xin Xiao Yue Xie Jian Zhu Jianfei Wang |
author_sort |
Cheng Zhou |
title |
Rhizobacterial Strain Bacillus megaterium BOFC15 Induces Cellular Polyamine Changes that Improve Plant Growth and Drought Resistance |
title_short |
Rhizobacterial Strain Bacillus megaterium BOFC15 Induces Cellular Polyamine Changes that Improve Plant Growth and Drought Resistance |
title_full |
Rhizobacterial Strain Bacillus megaterium BOFC15 Induces Cellular Polyamine Changes that Improve Plant Growth and Drought Resistance |
title_fullStr |
Rhizobacterial Strain Bacillus megaterium BOFC15 Induces Cellular Polyamine Changes that Improve Plant Growth and Drought Resistance |
title_full_unstemmed |
Rhizobacterial Strain Bacillus megaterium BOFC15 Induces Cellular Polyamine Changes that Improve Plant Growth and Drought Resistance |
title_sort |
rhizobacterial strain bacillus megaterium bofc15 induces cellular polyamine changes that improve plant growth and drought resistance |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2016-06-01 |
description |
Plant-growth-promoting rhizobacteria can improve plant growth, development, and stress adaptation. However, the underlying mechanisms are still largely unclear. We investigated the effects of Bacillus megaterium BOFC15 on Arabidopsis plants. BOFC15 produced and secreted spermidine (Spd), a type of polyamine (PA) that plays an important role in plant growth. Moreover, BOFC15 induced changes in the cellular PAs of plants that promoted an increase of free Spd and spermine levels. However, these effects were remarkably abolished by the addition of dicyclohexylamine (DCHA), a Spd biosynthetic inhibitor. Additionally, the inoculation with BOFC15 remarkably increased plant biomass, improved root system architecture, and augmented photosynthetic capacity. Inoculated plants also displayed stronger ability to tolerate drought stress than non-inoculated (control) plants. Abscisic acid (ABA) content was notably higher in the inoculated plants than in the control plants under drought stress and polyethylene glycol (PEG)-induced stress conditions. However, the BOFC15-induced ABA synthesis was markedly inhibited by DCHA. Thus, microbial Spd participated in the modulation of the ABA levels. The Spd-producing BOFC15 improved plant drought tolerance, which was associated with altered cellular ABA levels and activated adaptive responses. |
topic |
Bacillus megaterium polyamines drought tolerance abscisic acid Arabidopsis thaliana |
url |
http://www.mdpi.com/1422-0067/17/6/976 |
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