Time course of in vitro maturation of intra-erythrocytic malaria parasite: a comparison between Plasmodium falciparum and Plasmodium knowlesi
The schizont maturation assay for in vitro drug sensitivity tests has been a standard method employed in the global baseline assessment and monitoring of drug response in Plasmodium falciparum. This test is limited in its application to synchronous plasmodial infections because it evaluates the effe...
Main Authors: | , |
---|---|
Format: | Article |
Language: | English |
Published: |
Instituto Oswaldo Cruz, Ministério da Saúde
2002-09-01
|
Series: | Memórias do Instituto Oswaldo Cruz. |
Subjects: | |
Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762002000600028 |
id |
doaj-adb5552805764b2ea0431a66e01ca9ae |
---|---|
record_format |
Article |
spelling |
doaj-adb5552805764b2ea0431a66e01ca9ae2020-11-25T00:52:26ZengInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz.0074-02761678-80602002-09-0197690190310.1590/S0074-02762002000600028Time course of in vitro maturation of intra-erythrocytic malaria parasite: a comparison between Plasmodium falciparum and Plasmodium knowlesiSD SrinivasSK PuriThe schizont maturation assay for in vitro drug sensitivity tests has been a standard method employed in the global baseline assessment and monitoring of drug response in Plasmodium falciparum. This test is limited in its application to synchronous plasmodial infections because it evaluates the effect of drug on the maturation of parasite especially from ring to schizont stage and therefore synchronized P. falciparum cultures are required. On the other hand, P. knowlesi, a simian malaria parasite has a unique 24-h periodicity and maintains high natural synchronicity in monkeys. The present report presents the results of a comparative study on the course of in vitro maturation of sorbitol synchronized P. falciparum and naturally synchronous P. knowlesi. Ring stage parasites were incubated in RPMI medium supplemented with 10-15% pooled homologous serum in flat-bottomed 96-well micro plates using a candle jar at 37°C. The results suggest that the ideal time for harvesting the micro-assay plates for in vitro drug sensitivity test for sorbitol-synchronized P. falciparum and naturally synchronous P. knowlesi are from 26 to 30 h and from 22 to 25 h, respectively. The advantages of using P. knowlesi in chemotherapeutic studies are discussed.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762002000600028Plasmodium falciparumPlasmodium knowlesischizont maturation testin vitro drug sensitivity testsorbitol synchronization |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
SD Srinivas SK Puri |
spellingShingle |
SD Srinivas SK Puri Time course of in vitro maturation of intra-erythrocytic malaria parasite: a comparison between Plasmodium falciparum and Plasmodium knowlesi Memórias do Instituto Oswaldo Cruz. Plasmodium falciparum Plasmodium knowlesi schizont maturation test in vitro drug sensitivity test sorbitol synchronization |
author_facet |
SD Srinivas SK Puri |
author_sort |
SD Srinivas |
title |
Time course of in vitro maturation of intra-erythrocytic malaria parasite: a comparison between Plasmodium falciparum and Plasmodium knowlesi |
title_short |
Time course of in vitro maturation of intra-erythrocytic malaria parasite: a comparison between Plasmodium falciparum and Plasmodium knowlesi |
title_full |
Time course of in vitro maturation of intra-erythrocytic malaria parasite: a comparison between Plasmodium falciparum and Plasmodium knowlesi |
title_fullStr |
Time course of in vitro maturation of intra-erythrocytic malaria parasite: a comparison between Plasmodium falciparum and Plasmodium knowlesi |
title_full_unstemmed |
Time course of in vitro maturation of intra-erythrocytic malaria parasite: a comparison between Plasmodium falciparum and Plasmodium knowlesi |
title_sort |
time course of in vitro maturation of intra-erythrocytic malaria parasite: a comparison between plasmodium falciparum and plasmodium knowlesi |
publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
series |
Memórias do Instituto Oswaldo Cruz. |
issn |
0074-0276 1678-8060 |
publishDate |
2002-09-01 |
description |
The schizont maturation assay for in vitro drug sensitivity tests has been a standard method employed in the global baseline assessment and monitoring of drug response in Plasmodium falciparum. This test is limited in its application to synchronous plasmodial infections because it evaluates the effect of drug on the maturation of parasite especially from ring to schizont stage and therefore synchronized P. falciparum cultures are required. On the other hand, P. knowlesi, a simian malaria parasite has a unique 24-h periodicity and maintains high natural synchronicity in monkeys. The present report presents the results of a comparative study on the course of in vitro maturation of sorbitol synchronized P. falciparum and naturally synchronous P. knowlesi. Ring stage parasites were incubated in RPMI medium supplemented with 10-15% pooled homologous serum in flat-bottomed 96-well micro plates using a candle jar at 37°C. The results suggest that the ideal time for harvesting the micro-assay plates for in vitro drug sensitivity test for sorbitol-synchronized P. falciparum and naturally synchronous P. knowlesi are from 26 to 30 h and from 22 to 25 h, respectively. The advantages of using P. knowlesi in chemotherapeutic studies are discussed. |
topic |
Plasmodium falciparum Plasmodium knowlesi schizont maturation test in vitro drug sensitivity test sorbitol synchronization |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762002000600028 |
work_keys_str_mv |
AT sdsrinivas timecourseofinvitromaturationofintraerythrocyticmalariaparasiteacomparisonbetweenplasmodiumfalciparumandplasmodiumknowlesi AT skpuri timecourseofinvitromaturationofintraerythrocyticmalariaparasiteacomparisonbetweenplasmodiumfalciparumandplasmodiumknowlesi |
_version_ |
1725242400305053696 |