Temporal Stability of the Salivary Microbiota in Oral Health.
OBJECTIVES:Saliva is a biological fluid suitable for biomarker analysis, and differences in the salivary microbiota in oral health and disease have been reported. For such comparative analyses, time of sampling is critical since the bacterial composition may vary throughout the day, i.e., diurnal va...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2016-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC4723053?pdf=render |
id |
doaj-adc6bad30bf348d59e775cb4b3cf842f |
---|---|
record_format |
Article |
spelling |
doaj-adc6bad30bf348d59e775cb4b3cf842f2020-11-24T21:52:03ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01111e014747210.1371/journal.pone.0147472Temporal Stability of the Salivary Microbiota in Oral Health.Daniel BelstrømPalle HolmstrupAllan BardowAlexis KokarasNils-Erik FiehnBruce J PasterOBJECTIVES:Saliva is a biological fluid suitable for biomarker analysis, and differences in the salivary microbiota in oral health and disease have been reported. For such comparative analyses, time of sampling is critical since the bacterial composition may vary throughout the day, i.e., diurnal variation. The purpose of this study is to compare the salivary microbiome over time to determine the optimal time for sampling. DESIGN:Stimulated saliva samples were collected from 5 orally healthy individuals in 4 h intervals for 24 h, and collection was repeated 7 days later (number of samples per person, n = 12, total number of samples, n = 60). Salivary microbiota was analyzed using the Human Oral Microbe Identification using Next Generation Sequencing (HOMINGS), and statistical analysis was performed using the Kruskal-Wallis test with Benjamini-Hochberg's correction for multiple comparisons, cluster analysis, principal component analysis and correspondence analysis. RESULTS:From a total of 60 saliva samples, 477 probe targets were collectively identified with a mean number of probes per sample of 207 (range: 153-307). Little or no variation in microbial profiles within subjects was observed over time. CONCLUSIONS:Although there was considerable variation between subjects, microbial profiles within subjects were stable throughout a 24 hour period and after 1 week. Since there is little or no evidence of diurnal variation of the salivary microbiome, time of sampling of saliva is not critical for perturbation or other microbial studies.http://europepmc.org/articles/PMC4723053?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Daniel Belstrøm Palle Holmstrup Allan Bardow Alexis Kokaras Nils-Erik Fiehn Bruce J Paster |
spellingShingle |
Daniel Belstrøm Palle Holmstrup Allan Bardow Alexis Kokaras Nils-Erik Fiehn Bruce J Paster Temporal Stability of the Salivary Microbiota in Oral Health. PLoS ONE |
author_facet |
Daniel Belstrøm Palle Holmstrup Allan Bardow Alexis Kokaras Nils-Erik Fiehn Bruce J Paster |
author_sort |
Daniel Belstrøm |
title |
Temporal Stability of the Salivary Microbiota in Oral Health. |
title_short |
Temporal Stability of the Salivary Microbiota in Oral Health. |
title_full |
Temporal Stability of the Salivary Microbiota in Oral Health. |
title_fullStr |
Temporal Stability of the Salivary Microbiota in Oral Health. |
title_full_unstemmed |
Temporal Stability of the Salivary Microbiota in Oral Health. |
title_sort |
temporal stability of the salivary microbiota in oral health. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2016-01-01 |
description |
OBJECTIVES:Saliva is a biological fluid suitable for biomarker analysis, and differences in the salivary microbiota in oral health and disease have been reported. For such comparative analyses, time of sampling is critical since the bacterial composition may vary throughout the day, i.e., diurnal variation. The purpose of this study is to compare the salivary microbiome over time to determine the optimal time for sampling. DESIGN:Stimulated saliva samples were collected from 5 orally healthy individuals in 4 h intervals for 24 h, and collection was repeated 7 days later (number of samples per person, n = 12, total number of samples, n = 60). Salivary microbiota was analyzed using the Human Oral Microbe Identification using Next Generation Sequencing (HOMINGS), and statistical analysis was performed using the Kruskal-Wallis test with Benjamini-Hochberg's correction for multiple comparisons, cluster analysis, principal component analysis and correspondence analysis. RESULTS:From a total of 60 saliva samples, 477 probe targets were collectively identified with a mean number of probes per sample of 207 (range: 153-307). Little or no variation in microbial profiles within subjects was observed over time. CONCLUSIONS:Although there was considerable variation between subjects, microbial profiles within subjects were stable throughout a 24 hour period and after 1 week. Since there is little or no evidence of diurnal variation of the salivary microbiome, time of sampling of saliva is not critical for perturbation or other microbial studies. |
url |
http://europepmc.org/articles/PMC4723053?pdf=render |
work_keys_str_mv |
AT danielbelstrøm temporalstabilityofthesalivarymicrobiotainoralhealth AT palleholmstrup temporalstabilityofthesalivarymicrobiotainoralhealth AT allanbardow temporalstabilityofthesalivarymicrobiotainoralhealth AT alexiskokaras temporalstabilityofthesalivarymicrobiotainoralhealth AT nilserikfiehn temporalstabilityofthesalivarymicrobiotainoralhealth AT brucejpaster temporalstabilityofthesalivarymicrobiotainoralhealth |
_version_ |
1725877112545476608 |