Loop mediated isothermal amplification: An innovative gene amplification technique for animal diseases

• Main Authors: Pravas Ranjan Sahoo, Kamadev Sethy, Swagat Mohapatra, Debasis Panda
• Format: Article
• Language: English
• Published: Veterinary World 2016-05-01
• Series: Veterinary World
• Subjects: designed primers; loop-mediated isothermal amplification assay; transboundary animal diseases
• Online Access: http://www.veterinaryworld.org/Vol.9/May-2016/6.pdf

India being a developing country mainly depends on livestock sector for its economy. However, nowadays, there is emergence and reemergence of more transboundary animal diseases. The existing diagnostic techniques are not so quick and with less specificity. To reduce the economy loss, there should be a development of rapid, reliable, robust diagnostic technique, which can work with high degree of sensitivity and specificity. Loop mediated isothermal amplification assay is a rapid gene amplification technique that amplifies nucleic acid under an isothermal condition with a set of designed primers spanning eight distinct sequences of the target. This assay can be used as an emerging powerful, innovative gene amplification diagnostic tool against various pathogens of livestock diseases. This review is to highlight the basic concept and methodology of this assay in livestock disease.