Initial segmentation patterns of microspores and pollen viability in soybean cultured anthers: indication of chromosome doubling

• Main Authors: Milena Barcelos Cardoso, Eliane Kaltchuk-Santos, Elsa Cristina de Mundstock, Maria Helena Bodanese-Zanettini
• Format: Article
• Language: English
• Published: Instituto de Tecnologia do Paraná (Tecpar) 2004-09-01
• Series: Brazilian Archives of Biology and Technology
• Subjects: Glycine max; FDA; binucleate pollen grains; multinucleate pollen grains; spontaneous diploidization
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500005

Anthers obtained from flowers buds of soybean cultivar IAS-5 were cultured in two basal culture media (B5 and B5 long). Cytological examinations of the in vitro anthers were performed during the first 20 days of culture to assay the viability (by propionic-carmine and fluorescein diacetate tests) and the stage of development of pollen grains. The frequencies of viable pollen grains varied significantly between bud sizes on the propionic-carmine analysis. The basal culture media and bud size had no clear effect on the frequencies of binucleate symmetrical and multinucleate pollen grains. Chromosome counts of metaphasic microspores throughout the culture period showed microspores with higher ploidy level in addition to normal chromosome number (n=20).<br>Anteras obtidas de botões florais da cultivar IAS-5 de soja foram cultivadas em dois meios de cultura basais (B5 e B5 longo). Análises citológicas das anteras cultivadas in vitro foram realizadas durante os primeiros 20 dias de cultura, a fim de avaliar a viabilidade (por testes de carmim propiônico e FDA) e o estágio de desenvolvimento dos grãos de pólen. As freqüências de grãos de pólen viáveis variaram significativamente entre os tamanhos de botões florais na análise com carmim propiônico. O meio de cultura basal e o tamanho do botão floral não têm um claro efeito nas freqüências de pólens binucleados simétricos e multinucleados. Contagens cromossômicas de micrósporos metafásicos ao longo do período de cultura mostraram nível de ploidia superior ao normal (n=20).