Antifungal and antiproliferative activities of endophytic fungi isolated from the leaves of Markhamia tomentosa
Context: Plants harbor endophytes with potential bioactivity. Markhamia tomentosa (Benth) K. Schum ex. Engl. (Bignoniaceae) is reported to possess antioxidant, anti-inflammatory and anticancer activities. Objective: The antifungal and antiproliferative properties of endophytic fungi extracts and fra...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Taylor & Francis Group
2017-01-01
|
Series: | Pharmaceutical Biology |
Subjects: | |
Online Access: | http://dx.doi.org/10.1080/13880209.2016.1263671 |
id |
doaj-adfe640f311d4eb98a892b7d93a22c90 |
---|---|
record_format |
Article |
spelling |
doaj-adfe640f311d4eb98a892b7d93a22c902020-11-25T03:33:52ZengTaylor & Francis GroupPharmaceutical Biology1388-02091744-51162017-01-0155159059510.1080/13880209.2016.12636711263671Antifungal and antiproliferative activities of endophytic fungi isolated from the leaves of Markhamia tomentosaMutiat Ibrahim0Nutan Kaushik1Abimbola Sowemimo2Hemraj Chhipa3Trevor Koekemoer4Maryna van de Venter5Olukemi A. Odukoya6University of Lagos, College of Medicine campusThe Energy and Resources Institute (TERI), Indian Habitat CentreUniversity of Lagos, College of Medicine campusThe Energy and Resources Institute (TERI), Indian Habitat CentreNelson Mandela Metropolitan UniversityNelson Mandela Metropolitan UniversityUniversity of Lagos, College of Medicine campusContext: Plants harbor endophytes with potential bioactivity. Markhamia tomentosa (Benth) K. Schum ex. Engl. (Bignoniaceae) is reported to possess antioxidant, anti-inflammatory and anticancer activities. Objective: The antifungal and antiproliferative properties of endophytic fungi extracts and fractions from M. tomentosa were evaluated. Material and methods: Endophytic fungi were isolated from the leaves of M. tomentosa and identified by ITS-rDNA sequence analysis. The antagonistic effect of the fungal strains was investigated against pathogenic fungi viz, Fusarium oxysporum, Sclerotinia sclerotiorium, Rhizoctonia solani, and Botrytis cinerea using the dual culture assay for 5–7 days. Antiproliferative effect of the fungal extracts and fractions (3.91–250 μg/mL) on HeLa cancer cell line was tested and IC50 was calculated. Poisoning food assay and antifeedant activity against the pathogenic fungi and Spodoptera litura larvae, for 7 days and 2 h, respectively, was also tested at concentrations of 250, 500 and 1000 μg/mL. Results: Fungal endophytes Trichoderma longibrachiatum and Syncephalastrum racemosum were isolated from the leaves of M. tomentosa. Isolated endophytic fungal strains and solvent extracts showed MIC value of 1000 μg/mL against tested pathogenic fungi in the dual culture and poisoning food assays. Methanol fraction of S. racemosum isolate showed the most effective antiproliferative activity with IC50 of 43.56 μg/mL. Minimal feeding deterrent activity against S. litura larvae was also observed. Discussion and conclusion: These findings showed that the leaves of Markhamia tomentosa harbor strains of endophytic fungi with promising health benefits, and suggest their antifungal and antiproliferative effects against pathogenic fungi and HeLa cancer cell line.http://dx.doi.org/10.1080/13880209.2016.1263671trichoderma longibrachiatumsyncephalastrum racemosumpathogenic fungihela cancer cell linespodoptera litura larvae |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mutiat Ibrahim Nutan Kaushik Abimbola Sowemimo Hemraj Chhipa Trevor Koekemoer Maryna van de Venter Olukemi A. Odukoya |
spellingShingle |
Mutiat Ibrahim Nutan Kaushik Abimbola Sowemimo Hemraj Chhipa Trevor Koekemoer Maryna van de Venter Olukemi A. Odukoya Antifungal and antiproliferative activities of endophytic fungi isolated from the leaves of Markhamia tomentosa Pharmaceutical Biology trichoderma longibrachiatum syncephalastrum racemosum pathogenic fungi hela cancer cell line spodoptera litura larvae |
author_facet |
Mutiat Ibrahim Nutan Kaushik Abimbola Sowemimo Hemraj Chhipa Trevor Koekemoer Maryna van de Venter Olukemi A. Odukoya |
author_sort |
Mutiat Ibrahim |
title |
Antifungal and antiproliferative activities of endophytic fungi isolated from the leaves of Markhamia tomentosa |
title_short |
Antifungal and antiproliferative activities of endophytic fungi isolated from the leaves of Markhamia tomentosa |
title_full |
Antifungal and antiproliferative activities of endophytic fungi isolated from the leaves of Markhamia tomentosa |
title_fullStr |
Antifungal and antiproliferative activities of endophytic fungi isolated from the leaves of Markhamia tomentosa |
title_full_unstemmed |
Antifungal and antiproliferative activities of endophytic fungi isolated from the leaves of Markhamia tomentosa |
title_sort |
antifungal and antiproliferative activities of endophytic fungi isolated from the leaves of markhamia tomentosa |
publisher |
Taylor & Francis Group |
series |
Pharmaceutical Biology |
issn |
1388-0209 1744-5116 |
publishDate |
2017-01-01 |
description |
Context: Plants harbor endophytes with potential bioactivity. Markhamia tomentosa (Benth) K. Schum ex. Engl. (Bignoniaceae) is reported to possess antioxidant, anti-inflammatory and anticancer activities. Objective: The antifungal and antiproliferative properties of endophytic fungi extracts and fractions from M. tomentosa were evaluated. Material and methods: Endophytic fungi were isolated from the leaves of M. tomentosa and identified by ITS-rDNA sequence analysis. The antagonistic effect of the fungal strains was investigated against pathogenic fungi viz, Fusarium oxysporum, Sclerotinia sclerotiorium, Rhizoctonia solani, and Botrytis cinerea using the dual culture assay for 5–7 days. Antiproliferative effect of the fungal extracts and fractions (3.91–250 μg/mL) on HeLa cancer cell line was tested and IC50 was calculated. Poisoning food assay and antifeedant activity against the pathogenic fungi and Spodoptera litura larvae, for 7 days and 2 h, respectively, was also tested at concentrations of 250, 500 and 1000 μg/mL. Results: Fungal endophytes Trichoderma longibrachiatum and Syncephalastrum racemosum were isolated from the leaves of M. tomentosa. Isolated endophytic fungal strains and solvent extracts showed MIC value of 1000 μg/mL against tested pathogenic fungi in the dual culture and poisoning food assays. Methanol fraction of S. racemosum isolate showed the most effective antiproliferative activity with IC50 of 43.56 μg/mL. Minimal feeding deterrent activity against S. litura larvae was also observed. Discussion and conclusion: These findings showed that the leaves of Markhamia tomentosa harbor strains of endophytic fungi with promising health benefits, and suggest their antifungal and antiproliferative effects against pathogenic fungi and HeLa cancer cell line. |
topic |
trichoderma longibrachiatum syncephalastrum racemosum pathogenic fungi hela cancer cell line spodoptera litura larvae |
url |
http://dx.doi.org/10.1080/13880209.2016.1263671 |
work_keys_str_mv |
AT mutiatibrahim antifungalandantiproliferativeactivitiesofendophyticfungiisolatedfromtheleavesofmarkhamiatomentosa AT nutankaushik antifungalandantiproliferativeactivitiesofendophyticfungiisolatedfromtheleavesofmarkhamiatomentosa AT abimbolasowemimo antifungalandantiproliferativeactivitiesofendophyticfungiisolatedfromtheleavesofmarkhamiatomentosa AT hemrajchhipa antifungalandantiproliferativeactivitiesofendophyticfungiisolatedfromtheleavesofmarkhamiatomentosa AT trevorkoekemoer antifungalandantiproliferativeactivitiesofendophyticfungiisolatedfromtheleavesofmarkhamiatomentosa AT marynavandeventer antifungalandantiproliferativeactivitiesofendophyticfungiisolatedfromtheleavesofmarkhamiatomentosa AT olukemiaodukoya antifungalandantiproliferativeactivitiesofendophyticfungiisolatedfromtheleavesofmarkhamiatomentosa |
_version_ |
1724561224017903616 |